It's been a year since Poppy became the new queen of the Trolls, Branch has his true colors and became the new hero of the Trolls, and the two made peace between Trolls and Bergens. And now, a new surprise arrives for Branch as his family arrive at Troll Village. Plus, the Trolls are having a special music party. But what happens when a mean Bergen plans to stop the party? Join the Trolls on a brand new adventure!
- Anna Kendrick as Poppy; the newly crowned ruler of Troll Village who is Branch's love interest.
- Justin Timberlake as Branch; the newly crowned hero of Troll Village who is Poppy's love interest.
- Zooey Deschanel as Bridget; the former scullery maid of the Bergens. Later in the end of the film, she becomes the new queen of the Bergens.
- Christopher Mintz-Plasse as King Gristle Jr.; the new king of the Bergens.
- Patton Oswalt as Tommy; a faithful knight Bergen, and Gristle's best friend. He is in love with Laura.
- Amy Adams as Laura; a new Bergen scullery maid, and Bridget's best friend. She is in love with Tommy.
- Emily Osment as Zuna; a dark Troll who is a very famous and popular singer. Later in the film, she is revealed to be a Wolf Troll. Her personality is similar to Poppy's, only not as optimistic, naïve or dimwitted. She is, however, tomboyish, outgoing, brave, loyal, strong, adventurous, wild-hearted, friendly, mature, smart, cynical, and a total music lover.
- Zak Orth as Wacky;
- Seth MacFarlane as Albert;
- Ariel Winter as Lulli;
- Katy Perry as Sleaze;
- Yuri Lowenthal as Fixit;
- Meghan Trainor as Jodie;
- Gilbert Gottfried as Dimwit;
- Dan Castellaneta as Raymond;
- James Corden as Biggie; a large and friendly Troll.
- Kevin Michael Richardson as Mr. Dinkles; Biggie's pet worm.
- Gwen Stefani as DJ Suki; the Trolls' resident disc jockey.
- Ron Funches as Cooper; a giraffe-like Troll who can walk on four legs.
- Icona Pop as Satin & Chenille; a duo of fashion forward Troll twins who are conjoined by their hair.
- Kunal Nayyar as Guy Diamond; a Glitter Troll who is always naked.
- Walt Dohrn as:
- Smidge; a young female Troll with a masculine voice.
- Fuzzbert; a hair Troll with two legs.
- Cloud Guy; an anthropomorphic cloud.
- King Peppy; the former ruler of Troll Village who is Poppy's father. He was previously voiced by Jeffrey Tambor.
- Gordon Ramsey as Cook; a cold and rude Bergen who is the Chef's cousin. He heard about his cousin's death, so he decides to get revenge on the Trolls. It is later revealed that he and the Chef hate each other because the Chef was chosen to be the chef instead of Cook. In the end of the film, he redeemed himself and becomes the new chef of the Bergens.
- Quvenzhané Wallis as Harper; a very artistic Maker Troll who has heterochromia. She joins the Snack Pack sometime after the events of the first film.
- Ricky Dillon as Aspen Heitz; a Sporty Troll skitterboarder who is the leader of Troll Town.
- Stephanie Nadolny as Karma Spriggs; a Nature Troll who was said to be a huge fanatic of nature.
- Salma Hayek as Cybil Stone; a Guru Troll who is one of Aspen's friends. She is known to be the professional life coach of Troll Town.
- Rebel Wilson as Maddy Hipper; a Maker Troll who is Troll Town's professional hair stylist.
- Meg DeAngelis as Moxie Dewdrop; a Rainbow Troll who is the best dancer in Troll Town.
- Tom Kenny as Kyle Sparkledust; a Pop Troll who is the best chef in Troll Town.
- Kandee Johnson as Mandy Sparkledust; a Pop Troll and Kyle's twin sister who is the builder and creator of all of Troll Town's treasures.
- This is the first Trolls film created by Universal Pictures. The first film was created by 20th Century Fox.
- It is based on the mobile game of the same name.
- Branch is in his true colors like in the first film.
- Branch's younger sister Panna, and his mother Willow will make their debut in the film.
- This is the first time DJ Suki talks since she only has one line in the first film. Plus, she has a bigger part in this film.
- A few deleted characters from the first film join the movie.
- There is another town of Trolls called Troll Town.
- Mystical Trolls, like the Trollimals and Cooper, live in Troll Forest.
- Troll Village, Troll Town and Troll Forest were united together into Harmony Village, home of the Pop Trolls, with Aspen as Poppy's lieutenant, and Woody as her third-in-command.