A young neckerchief-wearing kitten named Poppy and her friends, Alma the bunny, Zuzu the puppy, Mo the mouse and Owl, (except Egbert, a villainous badger who wears different costumes) go on a big adventure anywhere to different locations by every vehicle they need that matches her neckerchief to find Alma's little brother, Chester, who is lost and in trouble.
- Cindy Robinson as Lara
- Cake Charles as Poppy Cat and Ellie
- Alicyn Packard as Alma
- Katie Leigh as Zuzu
- Donald King as Owl
- Stephanie Darcy as Mo
- Teresa Gallagher as Egbert and Gilda
- Danny Katiana as Rocket Cat
- Kodi Smit-McPhee as Ravi
- Carlos Alazraqui as Big Cat
- Lara Jill Miller as Mother Bumblebird
- Nancy Cartwright as Chester
The score was composed by Michael Giacchino and all songs performed by Joanie Bartels, except where noted *.
- "The Poppy Cat Theme Tune"* – Music by Oliver Davis
- "The Locomotion"
- "Happy Feet"
- "Happy Dancing"* – Performed by Cake Charles
- "Splish Splash"
- "My Favorite Keys"* – Performed by Alicyn Packard & Stephanie Darcy
- "Animal Crackers In My Soup"
- "Sillie Pie"
- "Teddy Bear, Teddy Bear"* – Performed by Alicyn Packard, Katie Leigh, Donald King, Stephanie Darcy & Kodi Smit-McPhee
- "The Martian Hop"
- "Swinging on a Star"
- "On the Road to Where We're Going"
- "Limbo Rock"
- "Poppy Cat's Anthem"* – Performed by Cake Charles, Katie Leigh, Alicyn Packard, Donald King, Stephanie Darcy & Nancy Cartwright
- "Put on a Happy Face" (end title)
- "Somewhere Over the Rainbow" (end title)
- This movie is to be rated PG-13.
- Poppy and Alma play their recorders on Joanie Bartels' hit single "Somewhere Over the Rainbow" during the end credits.
- Teresa Gallagher voiced Egbert and Gilda in both versions: UK and US.