- Bret Iwan as Mickey Mouse
- Russi Taylor as Minnie Mouse
- Tony Anselmo as Donald Duck, Huey, Dewey, and Louie
- Tress MacNeille as Daisy Duck
- Bill Farmer as Goofy, Pluto and Horace Horsecollar
- April Winchell as Clarabelle Cow
- Eric Bauza as Scrooge McDuck
- Frank Welker as Oswald the Lucky Rabbit
- Saki Fujita as Miku Hatsune
- Asami Shimoda as Rin and Len Kagamine
- Yu Asakawa as Luka Megurine
- Meiko Haigou as MEIKO
- Naoto Fuga as KAITO
- Megumi Nakajima as Gumi Megpoid
- GACKT as Kamui Gakupo
Mickey Mouse: Hiya, honorary Mouseketeers, I'm Mickey Mouse!
Minnie Mouse: And I'm Minnie Mouse! We've got some friends for newbies out there.
Donald Duck: I'm Donald Duck!
Daisy Duck: I'm Daisy Duck!
Goofy: I'm Goofy!
Clarabelle Cow: I'm Clarabelle Cow!