Mane 6's 16 Animals is a 2021 animated adventure action comedy film by Warner Bros. Pictures and Warner Animation Group. From the ponies and animals behind The Lego Movie, The Ant Bully, Happy Feet, Storks, Smallfoot, Legend of the Guardians: The Owls of Ga'Hoole and The Lightning Storm: Into the Lightning Ice.
- Tara Strong as Twilight Sparkle
- Ashleigh Ball as Applejack
- Ashleigh Ball as Rainbow Dash
- Andrea Libman as Pinkie Pie
- Andrea Libman as Fluttershy
- Tabitha St. Germain as Rarity
- Cathy Weseluck as Spike
- Kiefer Sutherland as Samson
- Martin Lawrence as Boog
- Kevin James as Otis
- Gary Oldman as Lord Shen, main reformed villain.
- Mel Gibson as Rocky
- Zach Braff as Chicken Little
- Jerome Ranft as Gamma, fourth reformed villain.
- Bob Peterson as Alpha, second reformed villain.
- Peter Dinklage as Mighty Eagle
- Ben Stiller as Alex
- Jim Sturgess as Soren
- Elijah Wood as Mumble
- Ewan McGregor as Valiant
- Ricky Gervais as Bugsy
- Delroy Lindo as Beta, third reformed villain.
- Bryan Cranston as Vitaly
- Tabitha St. Germain as Princess Luna
- Nicole Oliver as Princess Celestia
- Britt McKillip as Princess Cadance