After a few years, Manny and gang are enjoying their new home. Peaches, Louis, Sid, Leo, Granny, Crash and Eddie heading for the beach for some fun. They encounter a new group of pirates led by Captain Nak and take Sid as captive, they follow them to Pirate Island and rescuing Sid. Unknowining they are following them back home and plan to take over their home.
- Peaches (voiced by Keke Palmer): Daughter of Manny and Ellie, she serve as the leader of Sub-Zero Heroes for the film.
- Louis (voiced by Josh Gad): Peaches' best friend and a Molehog.
- Leo (voiced by Drake Bell): A silver Smilodon and son of Diego and Shira, he has his mother's fur color and father's eyes. Cool and a show off, he likes race his old man and hangs with Louis.
- Sid (voiced by John Leguizamo): A sloth and one of the main protagonists.
- Crash & Eddie (voiced by Sean William Scott & Josh Peck): Two twin possums, Ellie's adopted brothers and Peaches' uncles.
- Olivia (voiced by Niki Watkins): A yellow/tan spotted prehistoric puma (Pseudaelurus) and Leo's love interest, she love to sing all the time and pull pranks on anyone. Fun, immature and shrewd, she consider as "popstar with a pranking problem" and Shira doesn't like her.
Old members of the herd
- Manny (voiced by Ray Romano): A wooly mammoth and one of the main protagonists.
Pirates: After the death of Captain Gutt. His nephew Nak takes over and seeks revenge on the herd, they travel around the sea and plunder every treasure insight. Nak knows if they kidnapped Sid and his friend rescue him, they can follow them to the island and get revenge. In the climax of film, the pirates were winning and about to kill Manny. Peaches and Ellie start singing "We Are Family" and the pirates start getting scare, everyone on the island join in and ganging up the pirates. They retreated and head back to ship, Nak was the only one to go finish his mission and Manny launch him to deep sea. Nak is chased by a prehistoric shark (Megalodon) and paddeling a small ice boat.
- Captain Nak (voiced by Tom Kenny): A prehistoric ape (Gigantopithecus) and Gutt's nephew, he was abandon by his uncle and later end up on Pirate Island. Clever and short tempter when provoke, he takes on his uncle role as pirate captain and form his crew. He seeks revenge for his uncle's death and captured Sid as part of his plan, he wants to conquer every landmass for his pirates.
- Squint (voiced by Aziz Ansari): A prehistoric rabbit (Palaeolagus) and Nak's first mate, one of the three to surivived and join Nak. Overconfident, violent and the bad boy of the crew, he becomes Nak's right-hand man. It's not smart to call him "cute" or "cuddly", you have second to run before he makes you rabbit chow. Defeated by Leo with Crash and Eddie's help, he and others retreated back to the ship. Defeated by Manny with Louis's help, he and the others retreated to the ship.
- Gupta (voiced by Kunal Nayyar): A Bengali badger and Nak's flag, one the three to survived and join Nak.
- Elvis Icical (voiced by Dee Bradely Baker): A light-blue/white Cryolophosaurus with werid abillity to shoot and sneeze ice blast, he was freed from the ice by Nak and works for him now.
- Rio Pearls (voiced by Michelle Rodriguez): A golden prehistoric eagle (Haast's Eagle) and the aerial retriever, she fly the sky and hunt down her prey. Sarcastic, brash and quick-thinker, she prefer doing aerial tricks than her job and speaks with a Spanish Accent. Defeated by Peaches and later Ellie, she and the others retreated back to the ship.
- Flynn (voiced by Nick Frost): A elephant seal and the muscle of the crew, one of the three to survived and join Nak.
- Ripup (voiced by Doc Shaw): A blue prehistoric kangaroo (Procoptodon) and hip-hop personality, he takes over Raz's job as weapons expert and artillery expert. Funny, bit of a talker and talented, he can use his powerful legs to launch projectiles at enemies by kicking them. Also, he love sing and dancing hile stomping the ground. In the end of the film, he was left behide and stuck with the Sub Zero Heroes. Sid finds him a girlfriend, Daisy and he finally join the herd.
- Backpack: A giant prehistoric clam that carries the weapons and Ripup's pet.
- Chuckles (voiced by Steve Blum): A prehistoric hyena (Hyaenodon) and comedian, he love making jokes of everything and friends with Bites. Defeated by Eddie and Crash, he and the others retreated to the ship.
- Bites (voiced by Tara Strong): A tan/gray prehistoric wolf (Dire Wolf) and tracker expert, she can hide anyone long she has the "smell." Defeated by Olivia, she and the others retreated to the ship.
- Pirates of Pirate Island: They inhabit on a unknown island and living in a shack-like huts, they known Gutt very well and respect Nak.
- Krack (voiced by Mark Hamill): A Hyaenodon with a left scar eyebrow.
- Witty (voiced by Dee Bradely Baker): A prehistoric piranha (Megapiranha) that is trapped in ice-bowl fill with water, he makes threats to anyone that teaches his bowl. He speaks with a German Accent and carry around by Ozzy.
- Ozzy (voiced by Dee Bradely Baker): A maroon/purple Procoptodon and Witty's friend, he speaks with a Austrailian Accent and the one who kick out Ripout from the group. He is eaten by sharks when Sid accidently push him off the cliff.
- Bruce the Shark (voiced by Frank Welker): A prehistoric tigershark (Megalodon) that waits for prey to fall in the ocean, he follows the pirates everywhere.
- Gambling Glen (voiced by Tom Kenny): A prehistoric giant armadillo that cheats in dices and rolling way with his money (fruits, acorns and etc.), he always chase by Big James.
- Big James (voiced by Charlie Murphy): A grey rhinoceros-like Brontotherium and the bully from the block, he still mad Glen for cheating him in dice and hunting him down.
- Daisy: A pink/teal Procoptodon and Ripout's new girlfriend at the end of the film.
- Ra Sar (voiced by Charlie Adler): A crazy black/lime Dilophsaurus that was unfrozen by Scrat and keeps on persuding him as food, always talking about his life and telling how crazy it is. Try to trick the Louis to help him find his pet, but really he wants to eat him and gets shot in the air by Peaches. In the film, he almost caught Scrat and falls in a deep hole. He finally home and smash by Rudy's foot.
- The isolated island: All of the animals from the Ice Age lives on the island, Manny, Sid, Diego and the others try to enjoy living their.
- Pirate Island