- Neil Morrissey as Bob, Roley, Lofty and Farmer Pickles
- Kate Harbour as Wendy, Dizzy, Pilchard and Bird
- Rob Rackstraw as Spud, Scoop, Muck and Travis
- Harry Styles as Dougal
- Cheryl as Florence
- Ian McKellen as Zebedee
- Jim Broadbent as Brian
- Keith Wickham as Dylan
- Helena Bonham Carter as Ermintrude
- Shan Cothi as Ermintrude's singing voice
- Steven Kynman as Mr. Rusty
- Tim Whitnall as Mr. McHenry
- Jimmy Hibbert as Mr. Grimsdale
- Jessie J as ???, Dougal's girlfriend
- The Greatest Day - sung by the Casts of Bob the Builder and The Magic Roundabout 2
- Sign of the Times - sung by Dougal/Doogal
- Bleeding Love - sung by ???
- All Out of Love - sung by Bob, Dougal/Doogal, Wendy and Florence
- No One - sung by ???
- The Greatest Day (reprise) - sung by the Cast
- Bob the Builder Meets The Magic Roundabout 2/Transcript/UK Version
- Bob the Builder Meets The Magic Roundabout 2/Transcript/US Version
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