After Nightwing left as a sidekick, Batman now uses Ace as his sidekick to stop the villains from causing trouble in gotham.
- Bruce Wayne/Batman (voiced by Diedrich Bader) - a vigilante who has been training to become a hero for years, now he is a hero who stops crime from happening. He’s a serious man who has a dog as a sidekick.
- Ace The Bat-Hound (vocal effects by TBD) - a dog who was raised by Bruce, being trained hard enough to stop crime with his owner.
- Barbara Gordon/Batgirl (voiced by TBD) - a girl who had quite the history with Batman and Nightwing. She is now working for the helper of The Justice League.
- Com. James Gordon (voiced by TBD) - the father of Barbara and a helper of Batman. Ever since Bruce and him worked together, TBD.
- Alfred Pennyworth (voiced by Jim Ward) - the butler of Bruce Wayne, he helps out at everything Batman needs from him.
- Hannah Freetz (voiced by TBD) - a former rivial of Batgirl, now she is the manager of a theme park and moved on.
- The Joker (voiced by TBD) - TBD
- Dr. Harleen Quinzel/Harley Quinn (voiced by TBD) - a villain and a sidekick of The Joker. She is shown to be a love intreset of The Joker on her side.
- Pamela Isely/Poison Ivy (voiced by TBD) - a supervillain who gains the powers of any type of nature that she uses against superheroes and people who TBD.
See Here List of Batman And Ace: Hound Wolves
- This is the second time that Robin doesn’t appear in the Batman series.
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