Cartoon Heroes: Battle Mode

My Little Pony: Equestria Girls

 * Sunset Shimmer
 * Pinkie Pie
 * Sci-Twilight Sparkle
 * Rarity
 * Applejack
 * Rainbow Dash
 * Fluttershy

Looney Tunes

 * Bugs Bunny
 * Daffy Duck
 * Pepe Le Pew
 * Foghorn Leghorn
 * Lola Bunny
 * Speedy Gonzales
 * Penelope Pussycat
 * Wile E. Coyote

Sonic the Hedgehog franchise

 * Sonic the Hedgehog
 * Princess Sally Acorn
 * Miles “Tails” Prower
 * Cosmo the Seedrian
 * Knuckles the Echidna
 * Amy Rose
 * Shadow the Hedgehog
 * Rouge the Bat

Animaniacs

 * Minerva Mink

Darkwing Duck

 * Darkwing Duck
 * Steelbeak
 * Megavolt
 * Quackerjack
 * Liquidator
 * Bushroot
 * Negaduck

Ducktales

 * Launchpad McQuack
 * Gizmoduck

Tiny Toon Adventures

 * Fifi la Fume
 * Calamity Coyote
 * Buster Bunny

Disney Princess

 * Anna
 * Ariel
 * Princess Aurora
 * Belle
 * Cinderella
 * Princess Jasmine
 * Merida DunBroch
 * Fa Mulan
 * Pocahontas
 * Rapunzel
 * Snow White
 * Princess Tiana

DC Super Hero Girls

 * Wonder Woman
 * Harley Quinn
 * Bumblebee
 * Supergirl
 * Poison Ivy
 * Batgirl
 * Katana

Mixels

 * Flain
 * Krader
 * Volectro
 * Chomly
 * Tentro
 * Scorpi
 * Niksput
 * Globert

Friendship

 * Rainbow Dash and Foghorn Leghorn: 20% Cooler Rooster
 * Sunset Shimmer and Daffy Duck: Empathic Duck
 * Sci-Twilight Sparkle and Wile E. Coyote: Acme Chemistry of Science
 * Applejack and Speedy Gonzales: Viva de Apples
 * Fluttershy and Pepe Le Pew: Ele-Scent of Kindness
 * Pinkie Pie and Bugs Bunny: Sweet Carrots
 * Rarity and Lola Bunny: Fabulous Heartthrob of Hoops
 * Penelope Pussycat and Lola Bunny: Girl Power
 * Lola Bunny and Negaduck: Sweet Revenge
 * Wile E. Coyote and Lola Bunny: Scientific Basketball Game