The Toon-Night Show

The Toon-Night Show is a live-action/animation/puppet television series created by Seth MacFarlane and John Lasseter and featuring animated characters and puppet/animatronics characters from various studios. The series is set in a television studio in Hollywood.

Format
The premise of The Toon-Night Show is that Brain and Stewie (from Family Guy) are the hosts of a variety/talk show on ACME Studios. The show struck closely to the Muppet Show, House of Mouse, and Muppets Tonight format of various skits (mostly featuring the show's human guest star) interspersed with some sort of crisis occurring backstage.

Main Characters
Some familiar characters from other animated films, shows, and shorts appeared as members of the studio audience.
 * Brain Griffin and Stewie Griffin (both voiced by Seth MacFarlane) - Brain and Stewie serves as the hosts of the show since no one else wanted the job.
 * Mickey Mouse (voiced by Bret Iwan) - Mickey Mouse (along with Bugs Bunny) serves as one of the two television producers of the show.
 * Bugs Bunny (voiced by Jeff Bergman) - Bugs Bunny (along with Mickey Mouse) serves as one of the two television producers of the show.
 * Taz the Tazmanian Devil (voiced by Jim Cummings) -
 * Louis (voiced by Sergio Di Zio) - Louis (from Wayside) serves as the janitor of ACME.
 * The Toon Patrol (voiced by David Lander, Rob Paulsen, Charles Fleischer, June Foray, and Fred Newman) - They appear as Pete's assistant.
 * Carl Wheezer (voiced by Rob Paulsen) - Carl is often seen in cameos as a backstage crew member and has even made some audience cameos.
 * Phineas Flynn (voiced by Vincent Martella) - The young boy and inventor who shows off his inventions on the show.
 * Doc Brown (voiced by Dan Castellaneta) - Appears in the show.
 * Ferb Fletcher (voiced by Thomas Sangster) - Phineas' brother and assistant.
 * Roger Rabbit (voiced by Charles Fleischer) - A rabbit who serves as Rabbit's assistant.
 * Grumpy (voiced by Bill Farmer) - He serves as the janitor of ACME.
 * Kick Buttowski (voiced by Charlie Schlatter) - Kick does his stunt shows on the show.
 * Yakkity Yak (voiced by Lee Tockar) -
 * Guano (voiced by Gary Mack) - The scriptwriter of LilyMu who makes cameos as a crew member.
 * Minnie Mouse (voiced by Russi Taylor) -
 * Donald Duck (voiced by Tony Anselmo) - Donald (along with Daffy) serves as the production assistant of the show.
 * Daffy Duck (voiced by Jeff Bergman) - Daffy (along with Donald) serves as the production assistant of the show.
 * Rowlf the Dog (performed by Bill Barretta) - He makes appearances playing pianos in some acts.
 * Squidward Tentacles (voiced by Rodger Bumpass) - He is host of his own segment.
 * Statler and Waldorf (performed by Steve Whitmire and Dave Goelz) - Statler and Waldorf are usually seen heckling the show from the stage left balcony box.
 * Willie the Giant (voiced by Will Ryan) -
 * Peter Rabbit 
 * Toots the Horn from Bonkers
 * Baby Puss from The Flintstones
 * The Wickersham Brothers
 * Gnu from The Muppet Show
 * Ice Cream Man from Lilo and Stitch
 * Tom Tucker from Family Guy
 * The Tookie Tookie Bird from George of the Jungle
 * Ape from George of the Jungle
 * Yo-Yo Flamingo from Fantasia 2000

Other Characters
Other characters included:
 * Mike the Microphone (voiced by Rod Roddy) - An anthropomorphic toon microphone who serves as the show's announncer.
 * Huey, Dewey, and Louie (voiced by Tony Anselmo and Russi Taylor) - Young duck triplets who are Donald's nephews.
 * Buzzie, Flaps, Ziggy, and Dizzy (voiced by Jim Cummings, Jeff Bennett, Baron Davis, and Jess Harnell) - A group of four vultures that make up their own barbershop quartet.
 * Big Mean Carl (performed by Bill Barretta) - A shaggy monster who is in the category of Muppets eating other Muppets. He also makes audience appearances.
 * SpongeBob SquarePants (voiced by Tom Kenny) - SpongeBob SquarePants the sea sponge who blows bubbles for fun.