Marvel's Spider-Man: Webbed Champion

Marvel's Spider-Man: Webbed Champion (or simply Spider-Man: Webbed Champion) is Iago PUC’s second version of Marvel’s Spider-Man Season 2 airing of Disney XD, Disney Channel and Disney+. It follows the events of Season 1 and Season 2 episode: "School of Hard Knocks", and follows a new different storyline.

Premise
Still focusing on Peter Parker balancing his double life as a high school student and as the superhero Spider-Man, the season also focuses on Spider-Man’s new adventures with his friends (also consisting of his lover Mary Jane Watson / Tigra) following J. Jonah Jameson’s arrest. Along with episodes featuring Peter’s friends progressively becoming superheroes themselves after learning of his secret as Spider-Man, the season incorporates new episode arcs:
 * The City of Heroes arc, on which Peter puts his double life on track by becoming an intern in Stark Industries, whereas his friends progressively become new superheroes as they join Spider-Man’s battles against random threats in New York City, and they work to uncover the identity of the Master Planner, an enigmatic criminal with an unexplained grudge against Spider-Man.
 * The Family Business arc, on which Peter (after experiencing strange visions through the Neuro-Cortex) works on solving secrets about his family’s past while working to continue his late parents’ unfinished researches and scientific projects. A journey which leads him to a encounter with his long lost younger sister Teresa and the creation of new heroes who help him in his ordeals with supervillains, including Captain America's nemesis the Red Skull, who was responsible for the deaths of Peter's parents.
 * The Legendary Universes arc, a special multi-part crossover arc on which Spider-Man and his friends travel across dimensions to uncover the origins of his parents' projects and prepare to face the Regent, who is longing to obtain discarded technology of Oscorp from other realities to establish a new, but more iron-fisted order in New York. Spider-Man's journey leads him to four universes from different franchises (promo refers to them as "universes beyond Marvel") where he makes new allies: those consisting of Rey Skywalker and her new Jedi Alliance from the Star Wars saga, Optimus Prime and the Autobots from the Transformers series, Sonic the Hedgehog and his fellow Freedom Fighters and the Teenage Mutant Ninja Turtles.

Events in the Crossover Universes

 * The story of the featured Star Wars universe is set after the events of Star Wars: The Rise of Skywalker, on which the Resistance has freed the galaxy from the First Order's tyranny, and Rey has started a new generation of Jedi with help from Jedi Masters from other dimensions and training young children who are also connected to the Force.
 * The story of the featured Sonic the Hedgehog universe has elements and characters from the 1995 animated series (which features the Freedom Fighters led by Sally Acorn), the Archie Comics (which introduces Hope Kinbotor), Sonic Boom (which introduced Sticks the Badger), and the 2020 film (which includes Sonic's human friend Tom Wachowski).
 * The story of the featured Transformers universe has elements of Michael Bay's cinematic franchise and the Prime TV series franchise.
 * The story of the featured Teenage Mutant Ninja Turtles has elements of the 2019 crossover animated film Batman vs. Teenage Mutant Ninja Turtles and the final season of the 2003 TV series.

Champions
A group of young superheroes led by Spider-Man who assembled to defend their civilian society from random threats and become the next generation of the Avengers.
 * Spider-Man / Peter Parker (voiced by Robbie Daymond) - A teenager who was bitten by a genetically-modified spider during a field trip to Oscorp. He is also the leader of a new team of young heroes called the Champions. From the episode "City of Heroes, Part 3: American Cats" to the remainder of the series, Peter wears a new, upgraded version of his suit, which is powered by Vibranium technology provided for him by T'Challa / Black Panther.
 * Appearance: The same as in the original series. His second Spider-Man suit now resembles the one worn by Tom Holland’s character in the Marvel Cinematic Universe.
 * Tigra / Mary Jane Watson (voiced by Ashley Johnson) - Peter’s girlfriend and a student in Midtown High School-later-Horizon High School. She is an half-human, half Titanian Tiger hybrid (daughter of F.E.A.S.T. nurse Mary Watson and Titanian Tiger Soto (also known as Stark Industries scientist Dr. Phillip Watson)) who has the ability to shapeshift into a humanoid tigress with the abilities of Fire and Ice Breath, Hypnosis and (according to her teammates) the “strength of ten tigers combined” (provided by Superhuman Strength and Stamina, wall climbing, Superhuman leaping, unbreakable claws and teeth, and able to run and swim in incredible speed). She is revealed to have known of Peter's secret life as Spider-Man since he was bitten by a radioactive spider (a flashback reveals she was the only one other than Peter himself at the Oscorp field trip who noticed the spider biting him) and has been in love with him after his first heroic success. She first made a non-speaking cameo in Season 1 episode "Venom", where she was the Football team mascot of Midtown High. She later made her full appearance in "On the Tiger's Claws", on which she is shown to have dropped out of Midtown High to enroll at Horizon High, and eventually revealing her metahuman and superhero nature as Tigra.
 * Appearance: Her human form is the same as in Marvel's Spider-Man: Maximum Venom. Her Tigra persona has a bright red hair and green feline eyes and is based on Greer Grant’s variant in the original comics, but with the costume colored on blue with cat teeth in the belt and added with two golden bracelets and a small silver tiara with a tiger head-shaped sapphire.
 * Ghost-Spider / Gwen Stacy (voiced by Laura Bailey) - A student at Horizon High and one of Peter’s friends who become aware of his identity of Spider-Man. In Season 1, Gwen briefly took on the Spider-Gwen alias when the majority of New York was exposed to the Jackal's chemicals, but was later cured in the climax of the "Spider-Island" arc. In Season 2, Gwen regained her Spider Powers due to exposure to a relic called the Blood Gem and adopted the Ghost-Spider alias in "City of Heroes, Part 1: Spider-Gwen Returns".
 * Appearance: The same as in the original series, but now with a straight longer hair. Her Ghost Spider costume is the same as in the Season 2 episode "The Day Without Spider-Man".
 * Kid Arachnid / Spy-D / Miles Morales (voiced by Nadji Jeter) - A student of Horizon High and Spider-Man's primary partner-in-justice who was bitten by the Electrolis Arachnatis spider created by Raymond Warren. Spider-Man became a mentor to Miles. In "Kraven's Amazing Hunt," he was the first to discover Peter Parker is Spider-Man and promised not to tell anyone at school unless Peter would do it himself.
 * Appearance: The same as in the original series.
 * Spider-Girl / Anya Corazon (voiced by Melanie Minichino) - A top student at Horizon High and Gwen's best friend as well as one of Peter’s friends who become aware of his identity of Spider-Man. In Season 1, Anya gained Spider Powers when the majority of New York was exposed to the Jackal's chemicals in the "Spider-Island" arc. In Season 2, Anya regained her Spider Powers due to the experimental cure she took during the "Spider Island" incident and adopted the Spider-Girl alias.
 * Appearance: The same as in the original show. Her Spider-Girl guise is the same as in the original Season 2, but with fingerless gloves.
 * Ms. Marvel / Kamala Khan (voiced by Kathreen Khavari) - A teenage superhero fangirl who manifested her size/shape shifting abilities due to exposure to the Terrigen Mist and her status as a Inhuman descendant. Appearing as a member (later revealed to be a trainee only) of the Avengers, Ms. Marvel soon joined Spider-Man’s team. She is a close friend to Spider-Man, with whom (despite initially skeptical of him) she warmed up during a mission to rescue Captain America, Captain Marvel, Black Widow and Hulk from A.I.M..
 * Appearance: The same as in the original series' Season 2 episode "School of Hard Knocks".
 * Firestar / Screwball / Liz Allan (voiced by Natalie Lander) - One of Peter and Mary Jane's classmates at Midtown High School, where she is ranked class president. In Season 1, she operated as the internet celebrity prankster Screwball, though she developed a change of heart after Spider-Man saved her and Randy from Absorbing Man and Hammerhead in "Screwball Live". In Season 2, she became Firestar, a superheroine with Flight and Pyrokinetic abilities, due to exposure to a small active shard of the destroyed Blood Gem which duplicated Molten Man's powers. She also sports a nanotech particle suit which adapts to her powers and changes her hair color from brown to red to further hide her identity. She also mentions herself as a fan of She-Hulk, whose costume was a main influence for Liz to forge her own.
 * Appearance: The same as in the original show. Her Firestar suit is a one piece leotard version of the one worn by Angelica Jones in the comics with the mask covering most of her head (leaving only her hair, eyes and mouth visible), and with her hair changing from brown to red and having a small fire in the tip.
 * Wave / Michelle Jones (voiced by Alanna Ubach) - Liz Allan's best friend, a Midtown High School student and aspiring professional swimmer who (due to exposure to an Oscorp Super Soldier formula which was accidentally dropped by Spencer Smythe in a gym pool she was swimming at in Midtown High) developed water-bending abilities and superhuman speed running and swimming. Despite terrified with her mutations, Michelle became inspired by Peter's heroic actions as Spider-Man and became the new heroine Wave. Along with a Stark Industries nanotech wetsuit and a underwater-breathing mask, she also wields a pair of Atlantean nano-tech Escrima Sticks which can extend themselves into a pair of Adamantium swords and (when fused together as a bo staff) can extend itself into a trident which can fire heat beams and electro-blasts.
 * Appearance: Based on her counterpart from Ultimate Marvel: Earth-2.
 * Ant-Girl / Cassandra "Cassie" Lang (voiced by Jessica DiCicco) - Gwen's rather enthusiastic, but highly intelligent 8-year-old younger cousin who received a prototype Pym Particle suit after coming in contact with a nano-tech wrist device which duplicated the functions of her father Scott's Ant-Man suit. Her suit comes out of the device, which she uses to design and redesign her suit. She strongly loves her cousin and is shown to have a very soft relationship with Peter Parker and the rest of the teenaged heroes, who view her as a little sister. She is also a very close friend to Marcus Thompson's younger brother Jack, whom she occasionally nicknames "Jackie".
 * Appearance: Similar to her counterpart from Ultimate Marvel: Earth-2 and animated after the females in the show.
 * Stormranger / Teresa Parker (voiced by Mae Whitman) - Peter's long lost 13-year-old younger sister who was born eight months after her parents left Peter to the care of Uncle Ben and Aunt May. On the wake of her parents' death by the hands of the Red Skull, Teresa left the orphanage she grew up in and took up one of her father's secret projects, which includes technology resources from both Stark Industries and the Kree Empire. Soon after, she donned a new flight nanosuit (which includes the same functions of Tony Stark's Iron Man suit, as well as camouflage and phasing functions) with arm blades and optic beams and begun to operate as the new superheroine Stormranger in hopes of bringing Red Skull and his associates (including Norman Osborn) to final justice. While near to succeed, she crossed paths with Spider-Man, who revealed himself to her as her brother Peter and, after Red Skull's death and Norman Osborn's arrest, Teresa was given the courage to finally live a happy life with him and Aunt May. Although she still operates as Stormranger, Teresa also begun living the same lifestyle her brother does, including by enrolling as a student in Peter's former school in Midtown High.
 * Appearance: Visually a young girl with blond hair and blue eyes. Her Stormranger suit is a one piece leotard silver variant of the Ironheart suit in the comics added with platinum wings, a golden mask with sapphire visors and blue artificial hair.
 * Nova / Sam Alexander (voiced by Jack DeSena) - A teenager who was chosen to become a member of the Nova Corps like his father Jesse before him. He is a friend of Ms. Marvel and Kid Arachnid. He first appears in "Brainstorm", helping Spider-Man's friends deal with the riots orchestrated in New York by Otto Octavius (as the Master Planner) while Spider-Man and officer Watanabe directly confront Octavius in the Supervillain Holding Facility.
 * Appearance: The same as in Guardians of the Galaxy: Mission Breakout.
 * Inferno / Dante Pertuz (voiced by Sam Riegel) - A young Inhuman with Pyrokinetic abilities. He is said to have earned his powers and become a superhero one month before Peter Parker first became Spider-Man.
 * Appearance: Based on his looks in the Marvel Rising franchise, added with golden goggles.
 * American Knight / Kevin Hunt (voiced by Cameron Bowen) - A new student of Horizon High who sports his own prototype of the Iron Man suit which is inspired on Captain America's attire. Along with the same functions of Tony Stark's armor, Kevin's suit also has a Vibranium-powered blue construct projector which allows him to conjure a sword and shield for close self defense.
 * Appearance: Visually based on the character Kevin in Marvel Rising: Initiation. His American Knight suit is based on Harry Osborn's Patrioteer suit from Ultimate Spider-Man vs. the Sinister 6.
 * Lin Lie the Sword Master (voiced by Matthew Yang King) - One of Shang-Chi's first students and the last descendant of Fuxi who received his ancestor's ancient sword, which has mysterious magical powers.
 * Appearance: The same as in Marvel: Future Fight and animated after the males in the show.
 * The Lion King / Marcus Thompson (voiced by Jason Marsden) - A teenager who, after becoming the host of an alien lion's spirit, earned increased abilities similar to those of Black Panther's and the abilities to liberate fire solar energy from his eyes and hands and let out a sonic wave lion roar, operating as a lion-based superhero. The lion spirit is recognized by T'Challa / Black Panther as a spawn of the Egyptian wargod . In his off time, Marcus is a party entertainer who brings joy to children in orphanages and hospitals dressed as a cartoon lion called "Lyle the Happy Lion", impressing his high school crush-later-girlfriend Barbara Morse and clashing with the interests of his estranged older brother Flash Thompson, whom Marcus only prefers to address to by his real name Eugene.
 * Appearance: Visually a red-haired, green-eyed teenager. His Lion King armor is a lion-themed light brown variant of Black Panther's outfit in Ultimate Avengers 2 with a red mane. His Lyle the Happy Lion costume consists of his normal outfit added with red shoes, white gloves and a animatronic lion mask.
 * Speed / Jack Thompson (voiced by Katie Leigh) - Marcus' 9-year-old younger brother and Flash's youngest brother who due to exposure to an Kree power gem which duplicated Quicksilver's powers, manifested the ability to run into super-sonic speeds. He is a very close friend to Gwen's younger cousin Cassie Lang, who occasionally calls him "Jackie" and he usually blushes about it. Like Marcus, Jack also has a estranged relationship with Flash due to his previous bully behavior.
 * Appearance: Visually a blue eyed brunette boy. His Speed outfit is based on Thomas "Tommy" Shepherd's in the comics, but with short pant-legs and sleeves and a blue speedster googles.
 * Brawn / Amadeus Cho (voiced by Eric Bauza) - A 24-year-old young genius and college student who is described as Earth's Seventh Smartest Person and, due to partial exposure to Gamma Radiation, he had become an younger version of the Hulk (although he retains his original personality and voice).
 * Appearance: Visually the same as in the comics and animated after the male characters in the show.
 * Mantis (voiced by Hynden Walch) - A naive but innocent, genetically engineered alien girl with the abilities of Empathy, Astral Projection, Superhuman Leaping, Healing Factor and Plant Manipulation. Her consciousness initially inhabited the form of an mutated alien insectoid which Peter Parker's parents were experimenting on before being awakened by Peter, who developed a symbiotic, telepathic link with her. Later, she developed a more humanoid form by merging with a human clone body created by Viper to be part of Hydra's new clone army. After helping defeat Hydra, Green Goblin and Madame Masque, Mantis resumed to live in the jungle, although she would still come back to her master, since she is still linked to him. It is revealed in the "Legendary Universes" arc that the chemical substance which gave Mantis life is a sample of the Mutagen Ooze, the substance which created the Teenage Mutant Ninja Turtles and their teacher and adoptive father Master Splinter.
 * Appearance: Initially, a dark green insectoid with light green eyes, the body of a scarab, the head of a ladybug and mantis antennas. In her full form, she is visually a teenaged variant of Pom Klementieff's character in Guardians of the Galaxy Vol. 2 and Avengers: Infinity War, but with Medusa's eye design in Avengers: Black Panther's Quest and pointed, elven ears. She wears a barefoot two-piece dark green leotard, a leaf-made wristlet in her left arm and a blue flower in her hair.
 * AC-5 / Ace - An A.I. drone who is part of one of Richard and Mary Parker’s science projects. Despite not able to talk in human language, Peter and his friends are shown able to understand his beeping. He is also shown to have (as Gwen describes) a “very human personality”, which is represented by the Emoji-like images on his holographic face. It is revealed in the "Legendary Universes" arc that he is a AC astromech droid unit from the Jedi Order's dimension.
 * Appearance: Visually a blue sphere-shaped robot with white stripes, two plane wings, a camera lens in the left side of the forehead and a black screen with blue LED eyes.
 * Jarvis / J.A.R.V.I.S. (voiced by Patton Oswalt) - Richard Parker's old A.I. robot assistant who was stored in the main computers of the Triskelion before Richard and Mary were ambushed and killed by Red Skull. He was soon found and reawakened by his master's son Peter, who then downloaded Jarvis into the brain of a robot body which was part of the Parkers' second project. The robot uses the Tesseract (a cosmic-powered cube housing the Space Infinity Stone) as a power source and enhancer and has the functions of analyzing and identifying organic individuals (able to scan humans' identities and personal profiles) and create teleportation portals. It is revealed in the "Legendary Universes" arc that Jarvis' robot body was an discarded E-100 robot unit created by Dr. Robotnik.
 * Appearance: His robot form is visually based on E-102 Gamma in Sonic Adventure and Sonic X, but colored on white and blue with logos of the Avengers in the shoulders and with the head resembling Iron Man's helmet.
 * Arsenal / Chris Cooper (voiced by Christopher Daniel Barnes) - An army sergeant who was assumed killed by the Red Skull while leading his men to an assault on Hydra. Flashbacks reveal that he was vaporized by Red Skull with a Neuro-Cortex-powered gun which not only disintegrated his body, but absorbed his consciousness into a micro-card which ended up lost when Truman Marsh double-crossed Skull and Osborn for his own plans. The card with his consciousness was later recovered by Teresa Parker / Stormranger, who then delivered it to Spider-Man and his team and transferred Sergeant Cooper's consciousness into an alien robot body which Peter and Teresa's parents were working on as part of their third project. Now named "Arsenal", Sergeant Cooper earned the abilities to manipulate technology and change his body to the form of any vehicle he scans. He eventually took the shape of one of the Avengers' Quinjets. He now acts as a guardian to Spider-Man and his friends, including his daughter Carlie. It is revealed in the "Legendary Universes" arc that Sergeant Cooper's Arsenal body was made of the remains of an Cybertronian Seeker who was not affiliated to either Autobots or Decepticons.
 * Appearance: His human form (seen in flashbacks and holograms) is based on actor Bradley Cooper's interpretation of Chris Kyle in American Sniper. His robot form resembles Jetfire in Transformers: Fall of Cybertron, but with a red helmet resembling Ultra Magnus' in Transformers: Prime and rocket turbines in his back.

Individual

 * Alpha Owl / Carlie Cooper (voiced by Tara Strong) - A confident friend and fellow Stark Industries intern of Peter Parker and a keeper of his secret as Spider-Man, Carlie is a gifted science prodigy and inventor who specializes on the Neuro-Cortex, which she uses as the power source of her bird-based robots and drones; most notably a robotic owl named Edith, which can be used for researches and battles against crime and which she can also speak, hear and see through. She is shown to have a strong grudge with Otto Octavius, whom she blames for a lab accident which paralyzed her from the waist down and made her dependent of her wheelchair one year before Peter first became Spider-Man. She is also an ally of Spider-Man's team code-named the Alpha Owl, a high-tech wizard who monitors superheroes' activities through her computers (a role similar to Barbara Gordon / Oracle in DC Comics), and also enrolls as a student in Horizon High, where she earns a new advanced wheelchair with anti-gravity functions.
 * Appearance: A teenage version of her comic book counterpart with blue eyes. Her robotic owl Edith is visually based on a Barn Owl and colored on white and blue.
 * Aunt May (voiced by Nancy Linari) - Peter's aunt and sole guardian following her husband Ben's death. She eventually admits awareness of Peter's secret life as Spider-Man and confesses herself proud of what he became. In the end of the "Family Business" arc, she becomes aware of Teresa Parker's existence and, at the end of "Sons of Fathers", she pleads for Peter to bring her home next time he sees her. Later in "Seeds of Life", she is shown welcoming Teresa in open arms to her new home.
 * Appearance: The same as in the original show.
 * Max Modell (voiced by Fred Tatasciore) - The headmaster of Horizon High. He is shown to be aware and supportive of Peter's secret life as Spider-Man and acts as a supervisor to the Champions.
 * Appearance: The same as in the original story.
 * Robbie Robertson (voiced by Ernie Hudson) - A journalist-later-chief editor at the Daily Bugle who, unlike J. Jonah Jameson, has positive views of Spider-Man and sees him as a natural hero like the Avengers. He is also revealed to know of Peter's secret as Spider-Man and plays a role helping him and Mary Jane expose Jameson for his true colors.
 * Appearance: The same as in the episode: "Screwball Live".
 * Betty Brant (voiced by Grey DeLisle) - A journalist-later-chief editor at the Daily Bugle who, unlike J. Jonah Jameson, has positive views of Spider-Man and sees him as a natural hero like the Avengers. She is also revealed to know of Peter's secret as Spider-Man and is the one who alerts him of Jameson's hostile intentions against him after discreetly overhearing Jameson hiring Scorpion to intercept Peter and kill Spider-Man.
 * Appearance: The same as in the original story.
 * Cloak / Tyrone Johnson (voiced by Bumper Robinson) and Dagger / Tandy Bowen (voiced by Kari Wahlgren) - Two Inhuman teenagers whose power is greater when they are together; Cloak is capable of teleporting in a shroud of Darkforce energy whereas Dagger can generate dagger-like constructs out of Lightforce energy which can also be used to deal wounds and diseases. They primarily reside in Attilan like a majorly of the Inhumans, but they also spend time on Earth socializing with humans and undergoing superhero activities. They are also allies and friends to Peter Parker / Spider-Man, whom they helped with his parents' Richard and Mary Parker's scientific projects while Peter in turn helped them defeat Ronan the Accuser. They later appeared in "Legendary Universes, Part 6: Ultimate Alliance", where they joined Spider-Man and his allies in the battle against Regent's invading army.
 * Appearance: The same as in the original Season 2 story's episodes "Cloak and Dagger", "Brand New Day" and "The Cellar".
 * Hobgoblin / Harry Osborn (voiced by Max Mittelman) - Peter's best friend and a student at Osborn Academy. Throughout Season 1, Harry was resentful of Spider-Man, blaming Spider-Man for his expulsion from Horizon High, yet supportive towards Peter. Examples of this relationship included Harry blaming Spider-Man for Norman's 'death' in "The Rise of Doc Ock" Pt. 4 (while vowing that he and Peter will never fight like Herman Schultz and Clayton Cole in "Osborn Academy"). Harry eventually discovered best friend's secret identity during the "Spider Island" arc, putting his friendship with Peter at risk as he still blamed Spider-Man for his misfortunes. In Season 1's finale "Hobgoblin", Harry received the Hobgoblin technology from Norman and saved Peter from Octavius's mind control before ultimately saving his friend after seeing Norman's true colors. After his father's presumed death, Harry is Oscorp's acting CEO. After a long absence, Harry eventually returns in "Sons of Fathers", on which he helps Peter stop Donald Menken from selling Oscorp technology to Hydra, although Harry ends up feeling traumatized to hear of his father's shocking role in the deaths of his best friend's parents and Norman's alliance with the Red Skull.
 * Appearance: The same as the original.
 * Lady Octopus / Carolyn Trainer (voiced by Kari Wahlgren) - Otto's former accomplice and cellmate at The Cellar, having faked her criminal record so they could be friends. First appearing in "Between Ock and a Hard Place", Carolyn served as Doctor Octopus' accomplice under the Lady Octopus alias until after discovering Otto was merely using her to weaponize the Neuro Cortex. After using the machine to put Octavius in a coma, Carolyn discarded her tentacles as a sign of refusing to throw away her second chance. Unfortunately, it is later revealed in "Brainstorm" that Carolyn's actions inadvertently resulted in Octavius' consciousness being trapped in cyberspace. Later in "Real Wild Initiation", motivated by Spider-Man's latest actions resulting in Octavius being incarcerated on a mental hospital and Norman Osborn getting arrested, Carolyn felt encouraged to don the Lady Octopus once again to redeem herself from her crimes, eventually becoming an official ally to Spider-Man and the Champions. In the season finale "Legendary Universes, Part 6: Ultimate Alliance", she is among various heroes who join the Champions' battle against the Regent's army.
 * Appearance: The same as in the original.
 * She-Hulk / Jennifer "Jen" Walters (voiced by Maria Canals-Barrera) - A lawyer and district attorney and Bruce Banner's younger cousin who, due to an emergency blood transfusion from Banner, acquired a milder version of his Hulk condition, becoming a large, powerful green-hued version of herself while still largely retaining her voice and personality. Cheerful and optimistic, she is shown to have a good relationship with superheroes, such as Spider-Man. She was first mentioned in "City of Heroes, Part 2: Where There's Smoke" by Liz Allan, who idolizes She-Hulk and states to have modeled her Firestar suit after She-Hulk's purple leotard. She made her first official appearance in "The Parker Errand" (the first episode of the Season's "Family Business" arc), on which she and her fellow attorneys are building a case against J. Jonah Jameson during his trial, leading her to team up with Mockingbird (and later Spider-Man and Venom) in seeking evidences to prolong Jameson's prison sentence. She eventually succeeds by using evidences of Jameson's involvement with Richard and Mary Parker's death by the hands of Red Skull which lead Jameson to be in prison for life. She later appeared in "Legendary Universes, Part 6: Ultimate Alliance", where she and other heroes join forces with Spider-Man's team in defeating the Regent's army.
 * Appearance: The same as in the comics and sporting her A-Force leotard suit, and animated after the females in the show.
 * The Werefox / Katherine "Kit" Renner (voiced by Tara Strong) - An orphan residing at a F.E.A.S.T. shelter near Horizon High, and a Mutant/Inhuman hybrid with the skin and attributes of a red fox and the abilities to absorb metahuman energy with her teeth and claws. First appearing in "City of Heroes, Part 4: More Than Human", Spider-Man (who learned of her origins as a Mutant/Inhuman hybrid) came up with the objective to protect and look after Kit in order to preserve the peace treaty between Mutants and Inhumans. Later on, she was shown residing in Pegasus Z (a dimensional island discovered by the Titanian Tigers where homeless metahumans can reside freely without persecution) with Nocturnal as her guardian. Kit also has a passion for chess (which her father was a great expert at).
 * Appearance: The same as her counterpart in Ultimate Marvel: Earth-2.
 * Nocturnal (voiced by Yuri Lowenthal) - A young human who was transformed into a owl-like metahuman during a lab accident and became a recluse from fear of how the world would judge him. First appearing in "How I Thwipped My Summer Vacation", he lived out deep in the forest and stole food from people to survive. After stealing some food from Peter Parker and his friends while they were on a camping trip, Spider-Man confronted him and learned of Nocturnal's tragic past. Spider-Man befriended him but was unable to convince Nocturnal to meet his friends. Later on in "City of Heroes, Part 4: More Than Human", Nocturnal is shown acting as a superhero while explaining he had lost his fear of being seeing by the others following J. Jonah Jameson's arrest in "On the Tiger's Claws". He played a role helping Spider-Man and his allies protect the Werefox from Magneto and Maximus and preserve the peace treaty between Mutants and Inhumans. Growing as close to the Werefox like Spider-Man did, Nocturnal offered to look after her in Pegasus Z.
 * Appearance: The same as in the original.
 * Silk / Cindy Moon (voiced by Stephanie Sheh) - A Horizon High student who was bitten by a radioactive spider at the same day Peter was, and a Inhuman descendant whose Inhuman DNA increased her spider-powers to "a level beyond Spider-Man's" (enabling her with organic webbing, production of stingers in her arms, and Miles' abilities of camouflage and Venom Blasts). She first appears in "Web of Shadows" (the third seggment of female-centered episode "What Girl Are Made Of"), where she teams up with Gwen and Anya in dealing with Madame Hydra / Viper and stopping her plans against Inhumans.
 * Appearance: Visually the same as in the comics. As Silk, she wears a one top leotard version of her current comic book outfit. Additionally, in her Silk persona, she changes her eyes from human green to demonic red and summons a spider tattoo in her forehead.
 * TBD (voiced by TBD) - TBD
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 * Anna Maria Marconi (voiced by Katrina Kemp) - The dwarfish assistant chemistry teacher at Midtown High, and a known benefactor of the Inhuman Royal Family. She first appeared in "An Eye in the Sky", where she helped the Inhumans' head scientists with the fourth project (which would later give birth to Mantis) created by Peter Parker's parents, while Peter (as Spider-Man) helped Inferno, Silk, Cloak and Dagger to stop Ronan and MODOK.
 * Appearance: The same as in the original show.

Avengers
A group composed of world-reknowed superheroes (also given the honor of "Earth's Mightest Heroes"), who dedicate their lives protecting their world from several threats which no hero can fight alone.
 * Captain America / Steve Rogers (voiced by Roger Craig Smith) - The leader of the Avengers and a World War II veteran, who was enhanced to the peak of human physicality by an experimental serum and frozen in suspended animation before waking up in the modern world. He eventually becomes a mentor to Spider-Man and his friends.
 * Appearance: The same as in the original series' Season 2 episode "School of Hard Knocks" and in Avengers: Black Panther's Quest.
 * Iron Man / Tony Stark (voiced by Mick Wingert) - The second-in command and benefactor of the Avengers, who is the CEO of Stark Industries and a self-described genius, billionaire, playboy, and philanthropist with electromechanical suits of armor of his own invention. He is one of Peter's idols and mentors and a benefactor of Horizon High School.
 * Appearance: The same as in the original series.
 * Black Widow / Natasha Romanoff (voiced by Laura Bailey) - An Avenger and highly trained spy working for S.H.I.E.L.D..
 * Appearance: The same as in the original series.
 * Captain Marvel / Carol Danvers (voiced by Jennifer Hale) - An Avenger and a former U.S. Air Force pilot whose DNA was fused with that of Kree scientist Mar-Vell's during an accident, which imbues her with the powers of superhuman strength, energy projection and flight. She is Kamala Khan's superhero idol and inspiration.
 * Appearance: The same as in the Marvel Rising franchise, but with the costume of Brie Larson's character in Captain Marvel.
 * Thor (voiced by Travis Willingham) - An Avenger and the crown prince of Asgard, based on the Norse mythological deity of the same name.
 * Appearance: The same as in the episode "House of M" in Avengers: Black Panther's Quest.
 * Hulk / Bruce Banner (voiced by Fred Tatasciore) - An Avenger and a genius scientist who, because of exposure to gamma radiation, transforms into a monster when enraged or agitated.
 * Appearance: The same as in the original series.
 * Hawkeye / Clint Barton (voiced by Troy Baker) - An Avenger, S.H.I.E.L.D. agent and master archer.
 * Appearance: The same as in Avengers: Black Panther's Quest, but sporting his outfit from Avengers: Endgame.
 * Black Panther / T'Challa (voiced by James C. Mathis III) - An Avenger and the king of the African nation of Wakanda, who gained his enhanced strength by ingesting the Heart-Shaped Herb. He is also equipped with his Vibranium Armor, which absorbs kinetic energy and redirects it into a Sonic Wave Pulse.
 * Appearance: The same as in Avengers: Black Panther's Quest.
 * Ant-Man / Scott Lang (voiced by Josh Keaton) - An Avenger and former petty criminal who acquired a suit which allows him to shrink or grow in scale while also increasing in strength and communicate with ants. He is Cassie's father and Gwen Stacy's maternal uncle.
 * Appearance: The same as in Avengers: Black Panther's Quest.
 * Wasp / Hope Van Dyne (voiced by Kari Wahlgren) - An Avenger and the daughter of Hank Pym and Janet van Dyne who was handed down a similar suit and the Wasp mantle from her mother. She is Gwen Stacy's maternal aunt and Cassie's mother.
 * Appearance: Based on Evangeline Lilly's character in Ant-Man and the Wasp and Avengers: Endgame.
 * F.R.I.D.A.Y. (voiced by Sumalee Montano) - Stark's Artificial Intelligence system.
 * Appearance: None.

X-Men
A group of heroic mutants who banded together to fight random threats and preserve peace between Mutantkind and Humanity.
 * Professor X / Charles Xavier (voiced by Tom Kane) - A paraplegic, but one of the world's most powerful mutant telepaths. He is the founder of the X-Men and Xavier's School for Gifted Youngsters.
 * Appearance: Based on Patrick Stewart's character in the X-Men films.
 * Wolverine / Logan (voiced by Steve Blum) - A member of the X-Men and a Canadian mutant born with hyper-acute, animal-like senses, claws on his hands, and an accelerated healing factor which made it possible to implant a coating of the indestructible metal alloy Adamantium on his skeleton.
 * Appearance: Based on Hugh Jackman's character in the films.
 * Storm / Ororo Munroe (voiced by Danielle Nicolet) - A Wakandan mutant with the ability to manipulate weather at will.
 * Appearance: The same as in Wolverine and the X-Men, but animated after the females in the show.
 * Iceman / Bobby Drake (voiced by Troy Baker) - A Mutant with ice abilities.
 * Appearance: Based on Shawn Ashmore's character in the films.
 * Rogue / Anna Marie (voiced by Ali Hillis) - Iceman's girlfriend, Wolverine’s prodigy and a mutant with the ability to drain and absorb other humans and metahumans’ minds and abilities through physical contact at will.
 * Appearance: Based on Anna Paquin’s looks in the Live-in Action Trilogy.
 * Shadowcat / Katherine "Kitty" Pryde (voiced by Hynden Walch) - Mary Jane's maternal cousin, and a student and the hall monitor of Xavier's School for Gifted Youngsters, Kitty is a young mutant with the abilities to phase through matter and walk through solid objects (she can also fuse with and hide within the shadows of other living beings and subjects with this power), shape-shift into a humanoid Lynx with increased strength and speed and communicate with felines (both domestic and wild cats).
 * Appearance: The same as in the current appearances in Ultimate Marvel: Earth-2.
 * Beast / Dr. Hank McCoy (voiced by Travis Willingham) - A mutant with leonine attributes, prehensile feet and superhuman physical abilities. Hank acts as a teacher in Xavier's school and he builds inventions for troubled students and is the builder of the X-Jet.
 * Appearance: The same as in Wolverine and the X-Men, but animated after the males in the show and more occasionally wearing a tuxedo suit.

Inhuman Royal Family
A group of Inhumans who rule the city of Attilan.
 * Black Bolt (voiced by Fred Tatasciore) - The Head of the Inhuman Royal Family and King of Attilan, whose voice can cause destruction with the slightest whisper. While he cannot speak with his mouth, he can still speak with the others with his Telepathic abilities.
 * Appearance: The same as in Avengers: Black Panther's Quest.
 * Medusa (voiced by Vanessa Marshall) - The wife of Black Bolt and Queen of Attilan, who has the ability to control and move her hair.
 * Appearance: The same as in Avengers: Black Panther's Quest.
 * Crystal (voiced by Stephanie Sheh) - Medusa's sister, the Princess of Attilan and the youngest member of the Royal Family, who has the ability to control the elements of Earth, Wind, Fire and Water.
 * Appearance: The same as in Avengers: Black Panther's Quest, but with green eyes and larger hair.
 * Lockjaw - Crystal's 2,000-pound (910 kg) canine companion and an Inhuman dog with the teleportation power (as well as other abilities) who serves the Inhuman Royal Family as their escort and as a loyal protector.
 * Appearance: The same as in Marvel Rising.

S.H.I.E.L.D. (Strategic Homeland Intervention, Enforcement and Logistics Division)
A espionage, special law-enforcement, and counter-terrorism agency allied to the Avengers and the Champions which often deals with paranormal and superhuman threats.
 * Nick Fury (voiced by Phil LaMarr) - The director of S.H.I.E.L.D. who originally organized the Avengers, and continues to be a mentor for the team.
 * Appearance: Visually based on Samuel L. Jackson's character in the MCU.
 * Yuri Watanabe (voiced by Sumalee Montano) - The chief of New York's Police Department and a high-ranking commander in S.H.I.E.L.D. who works closely with Fury (a role similar to Maria Hill in the MCU).
 * Appearance: The same as in the original Season 2 story.
 * Mockingbird / Barbara "Barbie" Morse (voiced by Kari Wahlgren) - Watanabe's protegee and a young S.H.I.E.L.D. trainee and super spy who operates in a flight suit and is equipped with a pair of battle staves and night vision glasses. She is also a great admirer of the Avengers and idolizes Black Widow. She is Marcus Thompson's high school crush-later-girlfriend, who showed admiration of his work as a party entertainer, and begun dating him after she learned of his double-life as the Lion King.
 * Appearance: A younger version of her appearance in Avengers: EMH!, but with a one-piece leotard version of her suit, and golden artificial wings.
 * Henry "Hank" Pym (voiced by Jim Meskimen) - The maternal grandfather of Gwen Stacy and Cassie Lang, he is a S.H.I.E.L.D. entomologist, and physicist who became the original Ant-Man after discovering the subatomic particles which make the transformation possible. He also has history with Peter Parker's parents Richard and Mary, whom he helped with their secret science projects before they were killed by Red Skull.
 * Appearance: Based on Michael Douglas' character in the MCU.

Crossover Heroes and Allies
Heroes and allies from the crossover universes featured in the "Legendary Universes" arc.

New Jedi Order
A group formed by Rey to be the new generation of Jedi.
 * Rey Skywalker (voiced by Daisy Ridley) - A former scavenger from Jakku, a member of the Resistance, the last Jedi, apprentice of Luke Skywalker and Leia Organa, and Palpatine's granddaughter. She adopted the surname Skywalker to honor her mentors, and started a new generation of Jedi by recruiting Jedi Masters from dying alternate realities of the galaxy and training Force-sensitive children on the ways of a Jedi. Her Lightsaber's hilt can extend itself to the stature of a quarterstaff. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", she and her fellow Jedi Masters and Resistance allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. During the episode, she is primarily partnered with Spider-man himself, who at many moments wins her sympathy and blessing. Rey is notably Spider-Man's only female crossover partner.
 * Appearance: The same appearance as in the movies, with the same hairstyle as in The Last Jedi and a long dress version of her attire in The Rise of Skywalker.
 * Galen Marek (voiced by Sam Witwer) - A Jedi Master from an alternate dimension of the galaxy whose counterpart from Rey's native reality was adopted, raised, and apprenticed by Darth Vader after the death of his father at Vader’s hands during the Galactic Empire's age. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Jedi Masters and Resistance allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. During the episode, he is primarily partnered with Stormranger.
 * Appearance: Visually based on his counterpart's clone in Jedi Robes.
 * Orkos - A Wookiee and Jedi Master from a alternate reality of Kashyyyk who wields a pair of green wrist lightsabers. Like Chewbacca and other Wookiees, he does not talk, and only roars and growls. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Jedi Masters and Resistance allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units.
 * Appearance: Visually the same of any regular Wookiee, added with white Jedi robes.
 * Rahm Kota (voiced by Fred Tatasciore) - A Jedi Master from an alternate dimension who was rendered blind following a battle with the Galactic Empire in his dimension. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Jedi Masters and Resistance allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. During the episode, he is partnered with Lin Lie the Sword Master, as Kota views Lin Lie as a spirited warrior.
 * Appearance: The same as his counterpart in Star Wars: The Force Unleashed 2.
 * Roron Corobb - A male Ithorian Jedi Master from an alternate dimension whose counterpart from Rey's native reality was killed during the Clone Wars. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Jedi Masters and Resistance allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units.
 * Appearance: The same as his canon counterpart in Star Wars: Clone Wars.

Galactic Resistance / New Republic
A galactic military organization allied to the Jedi formed to maintain peace and freedom.
 * Finn (voiced by John Boyega) - A member of the Resistance / New Republic and a former Stormtrooper (FN-2187) who defected from the First Order. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Resistance members and Jedi allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. During the episode, he is partnered with Nova.
 * Appearance: The same as in Star Wars: The Rise of Skywalker.
 * Poe Dameron (voiced by Matthew Mercer) - A high-ranking X-wing fighter pilot and commander of the Resistance who later inherited the rank of General from the deceased Leia. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Resistance members and Jedi allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units.
 * Appearance: The same as in Star Wars: The Rise of Skywalker.
 * Jessika Pava (voiced by Laura Bailey) - A high-ranking X-wing fighter pilot of the Resistance who is now flight instructor. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", she and her fellow Resistance members and Jedi allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. During the episode, she is partnered with Firestar, who impresses her with her own flight skills.
 * Appearance: The same as in Star Wars: The Force Awakens.
 * BB-8 - A BB-series astromech droid who operated approximately thirty years after the Battle of Endor, belonging to Poe Dameron, whom he accompanied during his flights on his T-70 X-wing starfighter. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Resistance members and Jedi allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. During the episode, he and other droids befriend AC-5 / Ace.
 * Appearance: The same as in the sequel trilogy.
 * Chewbacca - A Wookiee and first mate of the Millennium Falcon and longtime friend of the deceased Han Solo. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Resistance members and Jedi allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units.
 * Appearance: The same as in the original.
 * Lando Calrissian (voiced by Billy Dee Williams) - A veteran of the Rebel Alliance, the original owner of the Millennium Falcon and an old friend of Chewbacca, Leia, Luke and Han. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Resistance members and Jedi allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. He has history with Peter Parker's parents Richard and Mary Parker, with whom he shared knowledge of the AC droid units before they were destroyed by the First Order, leaving only AC-5 / Ace alive.
 * Appearance: The same as in Star Wars: The Rise of Skywalker.
 * C-3PO (voiced by Anthony Daniels) - A humanoid protocol droid in the service of the Resistance. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Resistance members and Jedi allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. During the episode, he and other droids befriend AC-5 / Ace.
 * Appearance: The same as in previous media.
 * R2-D2 - An astromech droid in the service of the Resistance. Officially appearing in "Legendary Universes, Part 2: Webs of the Force", he and his fellow Resistance members and Jedi allies help Spider-Man's team defeat the Dark Nation and solve the origins of AC-5's original droid units. During the episode, he and other droids befriend AC-5 / Ace.
 * Appearance: The same as in previous media.

Freedom Fighters
A group of heroes led by Sonic the Hedgehog to protect Mobius from any threat to their world.
 * Sonic the Hedgehog (voiced by Michael Rosenbaum) - One of the residents and protectors of Mobius and the leader of the Freedom Fighters, he is a blue anthropomorphic hedgehog who can run at supersonic speeds and curl into a concussive ball, primarily to attack enemies. Officially appearing in "Legendary Universes, Part 3: Unlimited Speed", he and his fellow Freedom Fighters help Spider-Man's team defeat EggRobo and Dr. Robotnik and solve the origins of Jarvis' robot body. He is primarily partnered with Spider-Man himself, as they both respect each other's leadership style.
 * Appearance: The same as in current games, added with a bracelet based on Chip/Light Gaia's necklace in Sonic Unleashed.
 * Sally Acorn (voiced by Lacey Chabert) - Sonic's girlfriend and a anthropomorphic female chipmunk of Mobius. A talented martial artist and gifted genius, she also wields a pair of Ring Blades and can run at supersonic speeds via a Power Ring she owns. Officially appearing in "Legendary Universes, Part 3: Unlimited Speed", she and her fellow Freedom Fighters help Spider-Man's team defeat EggRobo and Dr. Robotnik and solve the origins of Jarvis' robot body. During the episode, she becomes a very good friend and partner to Mary Jane Watson / Tigra, as they both view each other as dedicated princesses and support each other's relationship with their teams' leaders.
 * Appearance: The same as in the Pre-Super Genesis Wave comic book continuity, added with white gloves and blue rings in her wrists.
 * Miles "Tails" Prower (voiced by Tara Strong) - Sonic's best friend and sidekick, he is an eight-year-old anthropomorphic yellow fox of Mobius with two tails (hence the nickname) and a mechanical, scientific genius who is able to use his twin tails to propel himself like a helicopter and fly. Officially appearing in "Legendary Universes, Part 3: Unlimited Speed", he and his fellow Freedom Fighters help Spider-Man's team defeat EggRobo and Dr. Robotnik and solve the origins of Jarvis' robot body. During the episode, he befriends and partners with Kevin Hunt / American Knight, who impresses him with his own genius intellect.
 * Appearance: The same as in current games.
 * Knuckles the Echidna (voiced by Khary Payton) - Sonic's other best friend and adventure partner, he is a red anthropomorphic echidna from Angel Island in Mobius with the ability to glide and climb up walls, and is a powerful fighter with superhuman strength due to his spiked hands. He serves as the guardian of the Master Emerald, a huge gemstone which controls the seven Chaos Emeralds. Officially appearing in "Legendary Universes, Part 3: Unlimited Speed", he and his fellow Freedom Fighters help Spider-Man's team defeat EggRobo and Dr. Robotnik and solve the origins of Jarvis' robot body. During the episode, he becomes more closely partnered with Lion King.
 * Appearance: The same as in current games.
 * Sticks the Badger (voiced by Misty Lee (similar to Squirrel Girl in Ultimate Spider-Man)) - Sally's neighbor and confident friend and a wacky feral jungle badger of Mobius, the only descendant of Jebediah Badger, and a force of nature in every way, but also a loyal member of the Freedom Fighters despite her average understanding with technology. Officially appearing in "Legendary Universes, Part 3: Unlimited Speed", she and her fellow Freedom Fighters help Spider-Man's team defeat EggRobo and Dr. Robotnik and solve the origins of Jarvis' robot body. During the episode, she becomes a very close friend and partner to Wave.
 * Appearance: The same as in Sonic Boom.
 * Nicole the Holo-Lynx (voiced by Kari Wahlgren) - Sally's best friend and surrogate sister, and a Mobian artificial intelligence affiliated to the Freedom Fighters. Initially having no actual form, she was able to create a complex holographically simulated body-based on that of a Mobian lynx projected from her hand-held thanks to a Power Ring. Officially appearing in "Legendary Universes, Part 3: Unlimited Speed", she and her fellow Freedom Fighters help Spider-Man's team defeat EggRobo and Dr. Robotnik and solve the origins of Jarvis' robot body. During the episode, she primarily monitors the heroes' mission alongside Carlie Cooper / Alpha Owl.
 * Appearance: The same as in the Archie Comics.

Autobots
A group of heroic Cybertronian Transformers who fight for the freedom of all sentient lifeforms.
 * Optimus Prime (voiced by Peter Cullen) - The noble and chivalrous leader of the Autobots. He transforms into a red and blue long-nose truck resembling a Peterbilt 379 (based on his vehicle mode in the live-action Transformers films). Officially appearing in "Legendary Universes, Part 4: Heroes in Disguise", he and his fellow Autobots help Spider-Man's team defeat Megatron and his Decepticon faction and Predaking's Kree Armada and solve the origins of Arsenal's robot body. During the episode, he is primarily partnered with Spider-Man himself, as they pay respects for each other's style of leadership.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * Arcee (voiced by Sumalee Montano) - A courageous female Autobot who transforms into a blue motorcycle resembling a Kawasaki Ninja 250R. Officially appearing in "Legendary Universes, Part 4: Heroes in Disguise", she and her fellow Autobots help Spider-Man's team defeat Megatron and his Decepticon faction and Predaking's Kree Armada and solve the origins of Arsenal's robot body. During the episode, she is mainly partnered with Inferno (a homage to Arcee's previous friendship with young human Jack Darby in Transformers: Prime).
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * Bumblebee (voiced by Josh Keaton) - A young Autobot scout-turned-warrior who transforms into a yellow and black sports car resembling a Chevrolet Camaro (based on his vehicle mode in the live-action Transformers films). Officially appearing in "Legendary Universes, Part 4: Heroes in Disguise", he and his fellow Autobots help Spider-Man's team defeat Megatron and his Decepticon faction and Predaking's Kree Armada and solve the origins of Arsenal's robot body. During the episode, he becomes a very close friend and partner to Ms. Marvel.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * Ratchet (voiced by Jim Meskimen) - A veteran Autobot medic who transforms into a white and red ambulance resembling a Ford E-350. Officially appearing in "Legendary Universes, Part 4: Heroes in Disguise", he and his fellow Autobots help Spider-Man's team defeat Megatron and his Decepticon faction and Predaking's Kree Armada and solve the origins of Arsenal's robot body. He is primarily partnered with Arsenal, as Chris Cooper mentions he was once army medic before he was appointed sergeant.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * Bulkhead (voiced by Kevin Michael Richardson) - A husky and cantankerous Autobot warrior and a devoted follower of Optimus Prime who transforms into a green SUV resembling a Lamborghini LM002 and is based on the Transformers: Animated character of the same name. Officially appearing in "Legendary Universes, Part 4: Heroes in Disguise", he and his fellow Autobots help Spider-Man's team defeat Megatron and his Decepticon faction and Predaking's Kree Armada and solve the origins of Arsenal's robot body. During the episode, he becomes partners with Brawn, who impresses Bulkhead with his show of strength.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * Grimlock (voiced by Travis Willingham) - The leader of the Dinobots (Cybertronians allied to the Autobots who transform into mechanical dinosaurs) who transforms into a grey, red and gold mechanical Tyrannosaurus which can breathe fire, and he wields a flaming sword in robot mode. Officially appearing in "Legendary Universes, Part 4: Heroes in Disguise", he and the Autobots help Spider-Man's team defeat Megatron and his Decepticon faction and Predaking's Kree Armada and solve the origins of Arsenal's robot body. During the episode, he forms a very close friendship with Speed and Ant-Girl, as Spider-Man remarks that Grimlock is "fulfilling children's classic dream of seeing a dinosaur".
 * Appearance: The same as in Transformers: Animated, and animated after the characters in Marvel's Spider-Man.

Teenage Mutant Ninja Turtles
Four fictional teenaged anthropomorphic mutant turtles named after Italian Renaissance artists who were trained by their anthropomorphic rat sensei Master Splinter in the Japanese martial art of ninjutsu.
 * Leonardo (Leo) (voiced by Eric Bauza) - The leader of the turtles who wields a pair of katana in battle. Officially appearing in "Legendary Universes, Part 5: Of Spiders and Turtles", he and his brothers help Spider-Man's team defeat the Spider Emperor and his clan of mutant animals and Shredder and his Foot Clan. He is mostly partnered with Spider-Man himself.
 * Appearance: The same as in Batman vs. Teenage Mutant Ninja Turtles, and animated after the characters in Marvel's Spider-Man.
 * Donatello (Don or Donnie) (voiced by Yuri Lowenthal) - The intelligent brother known for his scientific and technological expertise who builds equipment and machinery for the turtles and who wields a bo-staff in battle. Officially appearing in "Legendary Universes, Part 5: Of Spiders and Turtles", he and his brothers help Spider-Man's team defeat the Spider Emperor and his clan of mutant animals and Shredder and his Foot Clan. He is primarily partnered with Spider-Girl.
 * Appearance: The same as in Batman vs. Teenage Mutant Ninja Turtles, and animated after the characters in Marvel's Spider-Man.
 * Michelangelo (Mike or Mikey) (voiced by Greg Cipes) - The light-hearted brother known for his pranks and jokes and who wields a pair of nunchucks in battle. Officially appearing in "Legendary Universes, Part 5: Of Spiders and Turtles", he and his brothers help Spider-Man's team defeat the Spider Emperor and his clan of mutant animals and Shredder and his Foot Clan. He is primarily partnered with Ghost-Spider.
 * Appearance: The same as in Batman vs. Teenage Mutant Ninja Turtles, and animated after the characters in Marvel's Spider-Man.
 * Raphael (Raph) (voiced by Nolan North) - The hot-headed brother who wields a pair of sai in battle. Officially appearing in "Legendary Universes, Part 5: Of Spiders and Turtles", he and his brothers help Spider-Man's team defeat the Spider Emperor and his clan of mutant animals and Shredder and his Foot Clan. He is primarily partnered with Kid Arachnid.
 * Appearance: The same as in Batman vs. Teenage Mutant Ninja Turtles, and animated after the characters in Marvel's Spider-Man.

Crossover Supporting Characters

 * Din Djarin (voiced by Nolan North) - A gunfighter and bounty hunter from Mandalor who became allied to the New Republic following the fall of the First Order.
 * Appearance: Based on his looks in the Disney+ web miniseries The Mandalorian.
 * The Child - A toddler at age 50, raised by Djarin as his own, and a member of the same unnamed species as Yoda with similar Force abilities.
 * Appearance: Based on his looks in the Disney+ web miniseries The Mandalorian.
 * Tom Wachowski (voiced by Scott Porter) - The sheriff of Green Hills and Sonic's human friend.
 * Appearance: Based on his looks in the 2020 Sonic the Hedgehog film.
 * Maddie Wachowski (voiced by Kimberly Brooks) - A veterinarian of Green Hills and Tom's wife who is also a friend to Sonic and the Freedom Fighters.
 * Appearance: Based on her looks in the 2020 Sonic the Hedgehog film.
 * Hope Kintobor (voiced by Jessica DiCicco) - A 10-year-old orphan who is raised by the Wachowskis as their own, and views Sonic as a older brother.
 * Appearance: Based on her looks in the Archie Comics continuity.
 * Longclaw the Owl (voiced by Kari Wahlgren) - An anthropomorphic owl of Mobius who raised Sonic when he was young, and continues to be a mentor to him. She was also the creator of the Portal Rings which Sonic and his friends use.
 * Appearance: Based on her looks in the 2020 Sonic the Hedgehog film.
 * William "Will Bill" Lennox (voiced by Crispin Freeman) - An Army Rangers officer allied to the Autobots.
 * Appearance: Based on Josh Duhamel's character in Michael Bay's Transformers franchise.
 * Izabella Morgan (voiced by Melanie Minichino) - A teenager who is Lennox's protegee and a friend of the Autobots.
 * Appearance: Based on Hailee Steinfeld's portrayal of Charlie Watson in Bumblebee, with brown straight hair and blue eyes. She is most shown wearing a black sleeveless shirt, red shorts, brown boots, and a pair of fingerless long black gloves.
 * Swoop (sound effects are similar to those of the Pteranodons in Jurassic Park 3) - A Dinobot who transforms into a grey, red and gold mechanical Pteranodon and wields a flaming flail. He is the first who spots Spider-Man and his team during their arrival in the Transformers' dimension.
 * Appearance: The same as in Transformers: Animated, and animated after the characters in Marvel's Spider-Man.
 * Slug (sound effects and roars are similar to those of a bull) - A Dinobot who transforms into a grey and red mechanical Triceratops and wields a flaming club. He is best friends with Izabella, whom he is bent to protect.
 * Appearance: The same as in Transformers: Animated, and animated after the characters in Marvel's Spider-Man.
 * Special Agt. William Fowler (voiced by Phil LaMarr) - A government agent in Unit: E allied to the Autobots.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * April O'Neil (voiced by Catherine Taber) - A plucky, confident, clever, strong-willed, outgoing human companion of the Ninja Turtles, who embarked on many of the Turtles' adventures and also trained in the arts of Ninjútsu.
 * Appearance: The same as in Teenage Mutant Ninja Turtles: Back to the Sewer, and animated after the characters in Marvel's Spider-Man.
 * Casey Jones (voiced by Troy Baker) - The Turtles' second ally, April's lover, and a hockey-masked vigilante armed with an assortment of sporting goods which he carries in a golf bag.
 * Appearance: The same as in Teenage Mutant Ninja Turtles: Back to the Sewer, and animated after the characters in Marvel's Spider-Man.
 * Master Splinter (voiced by Richard Epcar) - The mutant rat sensei and adoptive father of the Ninja Turtles.
 * Appearance: The same as in the 2012 TV series, and animated after the characters in Marvel's Spider-Man.

Main

 * Doctor Octavius / Living Brain / Master Planner / Otto Octavius (voiced by Scott Menville) - The main antagonist of the "City of Heroes" arc, and minor antagonist in the second half of the show. He was a scientific prodigy who graduated from school early and became a teacher at Horizon High. Growing up, Octavius had a hard life between being picked on by a jock named Steve and having problems with his own abusive father Torbert. In Season 1's "The Rise of Doc Ock" arc, an accident by Crimson Dynamo caused his harness with tentacle-like appendages to be fused to his body, and he adopted the Doctor Octopus alias and turned the Osborn Commandos into the Sinister Five. His plans were foiled by Spider-Man and Hobgoblin in Season 1's finale "Hobgoblin". In Season 2 episode "Between Ock and a Hard Place", Octavius' body fell into a coma and his consciousness ended up in cyberspace to remain alive before relocating himself into the Living Brain. His plan to relocate himself into Spider-Man's body eventually backfired when Spider-Man's armor optics (powered by Vibranium provided for Spider-Man by Black Panther) absorbed Octavius' consciousness and returned him back to his inactive body. From there, Octavius (while incarcerated back to the Cellar) attempted (but failed) to remember the codifications of the Neuro-Cortex and be rid of his memories from the past, such as his shame of remembering a failed experiment which rendered Carlie Cooper paraplegic. In "Seeds of Life", he was broken out of his cell and brainwashed as Madame Masque's servant until Spider-Man's team and the Avengers defeated her, rendering her powerless and freeing Octavius, who was also rendered physically disabled and mentally ill in the progress.
 * Appearance: The same as in the original series. In "Seeds of Life", he is given a more roboticized look while under Madame Masque's control; added with a cyborg left arm, a cyborg red right eye and a power core (resembling Tony Stark's Arc Reactor) in his chest.
 * Red Skull / Johann Schmidt (voiced by Liam O'Brien) - One of the main antagonists in the "Family Business" arc. He is Captain America's arch-nemesis and the iron-fisted leader of Hydra. He played a role in the Parker family's history, as being responsible for killing Peter's parents Richard and Mary Parker.
 * Appearance: The same as in Avengers: Black Panther's Quest.
 * Green Goblin / Norman Osborn (voiced by Jeffrey Combs; replacing Josh Keaton in Season 1) - One of the main antagonists in the "Family Business" arc. He is Harry Osborn's father and Oscorp's original CEO. During Season 1, Norman secretly orchestrated his son's expulsion from Horizon High in the series premiere "Horizon High" in an attempt to make Harry as New York's definite hero, with Spider-Man's very existence being an interference with that vision. Although he was initially presumed dead following the "Rise of Doc Ock" arc, he resurfaced as the Spider King of the Jackal's Man-Spider army during the "Spider-Island" arc. In Season 1's finale "Hobgoblin", Norman utilized a replica Hobgoblin armor he made for his son so he can end Spider-Man himself, but was ultimately betrayed by Harry and was presumed dead following an explosion. He is revealed in Season 2 to have survived and gone into hiding with some of the technology he kept for himself from Oscorp. It is also revealed that he played a role in the deaths of Peter Parker's parents by turning them over to the Red Skull in retaliation of the couple having not agreed to work for him at Oscorp, inadvertently leaving Peter and his sister Teresa as orphans. Later reappearing in "Seeds of Life" as the Green Goblin, Osborn allied himself with Red Skull and his Hydra forces once more by working on human cloning using the cloning research he collected from Jackal. He was eventually defeated by Spider-Man and Stormranger, who teleported him back to New York. There, Osborn was devastated and enraged to know of his son Harry's death, and plotted revenge on Spider-Man and his friends. He consequently failed, and ended up defeated by the Champions while AC-5 used footage collected by Stormranger to expose Osborn's crimes and true colors to all over the city, leading to Osborn's life arrest and the end of Oscorp. Despite not appearing in the Season's last episodes, it is mentioned by Tigra that she induced Osborn with a mind spell which caused him to permanently hallucinate of revenants of the people he persecuted and betrayed, including Peter's parents Richard and Mary, and his own son Harry, leaving Osborn traumatized.
 * Appearance: The same as in Season 1. His Green Goblin attire is a light and dark green variant of his Hobgoblin suit.
 * Regent / Augustus Roman (voiced by Daran Norris) - The main antagonist in the "Legendary Universes" arc. He is Yuri Watanabe's former mentor and the former warden of the Cellar who was suspended from the Police Department for illegally experimenting on inmates' special abilities. Eventually forming an alliance with Alchemax, Roman donned a power suit of Alchemax technology which replicates the experimented inmates' abilities, and seeks to bring a new order, but iron fisted to New York.
 * Appearance: The same as in the original Season 2 story.

A.I.M.
An terrorist scientific organization created to overthrow high tech companies like Stark Industries and Horzion Labs and bring New York to their knees.
 * MODOK / George Tarleton (voiced by Charlie Adler) - A cybernetically-enhanced life form whose name is short for Mental Organism Designed Only for Kaos, with the mind and consciousness of former Hydra scientist George Tarleton. He is A.I.M.'s current leader.
 * Appearance: The same as in "A Troubled Mind" from the original story.
 * Truman Marsh (voiced by John DiMaggio) - An executive of Alchemax and benefactor of Oscorp who acts as A.I.M.'s head engineer. Though he did not have much of a role in the deaths of Richard and Mary Parker, he did carry evidence of J. Jonah Jameson covering the truth about the incident for money protection, a fact which was (in Present Day) exposed by Spider-Man, Mockingbird and She-Hulk.
 * Appearance: The same as in Avengers: Ultron Revolution.

Hydra
A terrorist Nazi group formed by the Red Skull to rule over humanity by any means.
 * Viper / Madame Hydra (voiced by Erica Luttrell) - A high-ranking general of Hydra who, after injecting herself with an stolen Super Soldier serum combined with Dr. Curt Connors' Lizard formula, became able to shape-shift into a monstrous humanoid snake with increased strength, a large snake tail replacing her right arm and the ability to spit acid venom.
 * Appearance: The same as in  Ultimate Marvel: Earth-2.
 * Crimson Widow / Yelena Belova (voiced by Julie Nathanson) - The latest graduated student in the Red Room program, and the second assassin to earn the name "Black Widow".
 * Appearance: The same as in Avengers: Black Panther's Quest.
 * Crossbones / Brock Rumlow (voiced by Fred Tatasciore) - An high-ranking agent and assassin of Hydra armed with a nearly indestructible combat armor and a pair of strength-enhancing shockwave gauntlets.
 * Appearance: The same as in the original.
 * Arnim Zola (voiced by Mark Hamill) - A Nazi biochemist during World War II who survived into the modern age by transferring his conscious mind into the body of a sophisticated robot which protected it by storing it in its chest and displaying a digital image of Zola's face on its chest plate.
 * Appearance: The same as in Avengers: Black Panther's Quest.

Athena Industries
A formerly small company which mainly focused in building innovative tech and making the world a better place which ended up becoming a corrupt agency seeking to overthrow all other companies, including Stark Industries and Oscorp.
 * Athena Gonzales (voiced by Nika Futterman) - The corrupt head of Athena Industries who longs to use technology to become the richest woman in the world, also wanting to outrank rich businessmen like Tony Stark and Norman Osborn. She is shown to have a mental illness which overwhelms her with a god-complex, by which she believes herself to be the dark reincarnation of the same name Greek goddess of wisdom Athena.
 * Appearance: Visually a straight long-haired brunette with hazel eyes. She is most seen wearing a outfit based on ancient greek dresses and a leaf tiara.
 * Techpire / Ariel Haven (voiced by Rachael MacFarlane) - A socially awkward scientist who created a serum which gave her the power to corrupt technology, using it to steal designs for Athena Industries. She is shown to be highly infatuated with Ant-Man, much to the objections of Cassie Lang.
 * Appearance: Visually based on actress Mary Elizabeth Winstead. Her Techpire form has a grey skin, black hair and red demonic eyes and sports a one piece jumpsuit detailed with cybernetic features.
 * Draco - An artificial dragon-like humanoid android named after the, which in Greek Mythology, was originally the Gigantomachian Dragon which Athena fought during the Gigantomachy.
 * Appearance: Based on Dragon Man in Ultimate Spider-Man.
 * Gabby Flenkman (voiced by ) - First appearing in "Rise Above All", she was the Wake Riders' publicist, who covered them as "heroes" even after Spider-Man and Kid Arachnid exposed them. She later appears in "The Calm Before the Stormranger", where she is now Gonzales' assistant. Spider-Man is shown to develop a reasonable distrust in her for backing the Wake Riders.
 * Appearance: The same as in the original.
 * Dr. Joseph Rockwell (voiced by Greg Ellis) - Athena Industries' head scientist and the creator of Gonzales' super soldiers and beast machines.
 * Appearance: The same as in the original Season 2 episode "The Road to Goblin War".
 * The Griffin / Johnny Horton (voiced by Fred Tatasciore) - Athena Industries' "beast machine super soldier", and a surgically-altered and cybernetically modified super-villain resembling a griffin.
 * Appearance: The same as in The Avengers: Earth's Mightiest Heroes!.

New Sinister Six
A new variation of the Sinister Six organized by the Jackal with the intend to fill the power void left after the fall of Oscorp.
 * The Jackal / Raymond Warren (voiced by John DiMaggio) - An animalistic, green-furred criminal. One of Season 1's central antagonists, he is portrayed as Gwen's uncle, and the creator of the spiders behind Peter's and Miles's Spider abilities. In his earlier life, Raymond was a former staff member at Empire State University who was dismissed for his genetic experiments. In addition to his specialty in hybridization, Raymond is capable of generating clones of himself, one served as a decoy in "Party Animals" to fool Spider-Man and the authorities into believing he was caught. During the "Rise of Doc Ock" arc, it is revealed that he conspired with Doctor Octopus and fully intended to use the genetically modified spiders to create an entire army at his command. His master plan unintentionally happened during the "Spider-Island" arc with the Man-Spider army but was ultimately thwarted by the Spider-Men, Anya and Harry.
 * Appearance: The same as in the original show.
 * Silver Sable / Silver Sablinova (voiced by April Stewart) - The former leader of the Wild Pack mercenary group. In "Take Two", Sable and her team attempted to steal the Neuro Cortex before being defeated by Spider-Man and Doctor Octopus. It is later revealed in "Between an Ock and a Hard Place" that the Wild Pack was hired by Doctor Octopus. In "Brand New Champions", Silver Sable returns as a member of the Jackal's new Sinister Six (viewed by Spider-Man's team as "the Six's own Black Widow").
 * Appearance: The same as in the original show.
 * Electro / Max Dillon (voiced by Troy Baker) - A super-criminal with electric abilities.
 * Appearance: Based on his counterpart from Ultimate Marvel, sporting the costume worn by Jamie Foxx's character in The Amazing Spider-Man 2.
 * Spider-Slayer / Alistair Smythe (voiced by Jason Spisak) - An inventor of the Spider-Slayers and Spencer Smythe's son. In "Party Animals", he auditioned for a scholarship at Osborn Academy by testing his Spider-Slayer against Spider-Man. During Season 1's second half, Alistair joined the Osborn Commandos before being brainwashed by Doctor Octopus and later freed by Hobgoblin.
 * Appearance: The same as in the original show, with his armor suit from the "The Rise of Doc Ock" arc and the two-part Season 1 finale "Hobgoblin".
 * Molten Man / Mark Raxton (voiced by Charles Halford) - A supervillain with fire abilities.
 * Appearance: The same as in the original Season 2 episode "Brand New Day".
 * Scorpion / Mac Gargan (voiced by Jason Spisak) - A sadistic scorpion-armored super-criminal. In the series premiere "Horizon High", Spider-Man stopped Scorpion from robbing the science museum. In "Kraven's Amazing Hunt", Scorpion was used as bait to hunt the Spider-Men in an episode of Kraven's show. He later appeared as a minor antagonist in Webbed Champion, starting with "On the Tiger's Claws", where he is hired by Jameson to kidnap Peter and the key figures of his life to draw Spider-Man out, although Scorpion ends up having traumatic encounters with Tigra, who effortlessly defeats him and cripples him.
 * Appearance: The same as in the original.

Individual

 * TBD (voiced by TBD) - TBD
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 * TBD (voiced by TBD) - TBD
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 * TBD (voiced by TBD) - TBD
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 * TBD (voiced by TBD) - TBD
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 * Jack O'Lantern / Jason Macendale (voiced by James Arnold Taylor) - A former Midtown High School jock who was kicked out of the school's football team by Flash Thompson for dressing as a clown in a children's hospital. Feeling humiliated, Macendale was approached by the Master Planner, who gave him equipment and weapons based on Harry Osborn's HobGoblin technology, which Macendale used to become Jack O'Lantern.
 * Appearance: His civilian look is based on Tony Revolori's portrayal of Flash Thompson in Spider-Man: Homecoming. As Jack O'Lantern, he is based on the character seen in the Canon Season's episode "Bring On the Bad Guys, Part 2".
 * Kraven the Hunter (voiced by Troy Baker) - A maniacal hunter with interests in hunting exotic animals who hosts the TV show Kraven's Amazing Hunt. In the episode "Kraven the Hunter", Kraven was hired by Norman to capture the Spider-Men for experimentation following the Spider-Slayers' repeated failures, only to betray Osborn and Vulture and be defeated by the two Spider-Men. In "Spider-Island" Pt. 3, Kraven allied with Peter, Anya and Harry in combating the first Man-Spiders. When he unsuccessfully betrayed the group by hunting the first two Man-Spiders, Kraven was defeated once more. In "On the Tiger's Claws", it is mentioned that Kraven had once hunted Tigra in her first superhero night, but failed miserably, resulting on him losing his left eye and right arm.
 * Appearance: The same as in Season 1.
 * J. Jonah Jameson (voiced by ) - The Daily Bugle Communications' head, John's father, and the Champions' regular critic. One such example is deeming Spy-D as a third-rate menace-in-training. Peter worked for Jameson as a freelance photographer, making an improvement on proving footage of Spider-Man compared to Eddie, from "Take Two" until "On the Tiger's Claws" where he hired Scorpion to retrieve Peter (after he quit the Bugle following a heated argument with a jealous Brock) and dispose of Spider-Man for good. After Venom and Scorpion were defeated by Spider-Man and Tigra, Jameson was exposed by Skrull general Talos and his young scouts Amon and Jade for involvement with the incident and arrested. During the "City of Heroes" arc, his arrest soon inspired other young heroes to act freely in New York while also exposed the Wake Riders (whom Jameson wrongly pointed as heroes) as true villains and accomplices of the Vulture and the Shadow Council. He is no longer seen again until "The Parker Errand", where it was revealed that Jameson also played a role in Richard and Mary Parker's death by Red Skull's hands by covering the truth about their demise as a "tragic, unexplainable accident", until Spider-Man, Mockingbird and She-Hulk sought evidences from Truman Marsh which led Jameson to be sentenced for life imprisonment.
 * Appearance: The same as in the original.

Main

 * EggRobo (voiced by Corey Burton) - An egg-shaped humanoid robot which resembles his creator Doctor Robotnik. Initially designed by Robotnik to be his lieutenant, EggRobo went rogue and tried to take over Robotnik's technologies, forcing Robotnik to shut him down. He was eventually reanimated by discarded prototypes of Doctor Octopus' tentacle harness, and now seeks to take over Robotnik's machines and roboticize humans and other organic life forms.
 * Appearance: The same as in Sonic & Knuckles, and animated after the characters in Marvel's Spider-Man and added with Doctor Octopus' tentacle harness.
 * Predaking (voiced by Ike Amadi) - The leader of the Predacons (a race of ancient Cybertronian dragons which have been long extinct), who transforms into a black and orange mechanical dragon which resembles depictions of such creatures in European folklore. Initially awakened by Megatron and experimented on by Shockwave to be the Decepticon's "beast machine super-soldier", Predaking went rogue and attempted to overthrow his captors, resulting in Megatron forcibly killing him and leaving his remains in a uninhabited area of Earth. Predaking was soon resurrected by energies from the Kree Empire's power capsules which were discarded by Oscorp engineers years ago, also allowing him to create and control an army of Kree machines along with his own version of the Dark Aster. He now seeks to convert humans' technology to his own liking and take revenge on Megatron and his Decepticon followers.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * The Spider Emperor (voiced by Fred Tatasciore) - The leader of a Ninja Clan which was been rivals with the Foot Clan. He and his clan were mutated into Animal hybrids by samples of the Jackal's animal formulas. The leader was transformed into the Spider Emperor (similar to the people of Spider-Man's New York during the Man-Spider incident), and unlike the rest of his clan, he retained his original personality and intelligence, acting as the clan's "alpha".
 * Appearance: His mutated form is based on Norman Osborn's Spider King form in episode "Spider-Island, Part 4". His human form resembles Shang Tsung in Mortal Kombat 11.

Dark Nation
A cult-like organization formed by former members and loyalists of the First Order determined to bring the Galaxy to their knees.


 * Captain Phasma (voiced by Vanessa Marshall) - A former high-ranking member and commander of the First Order who serves as the leader of the Dark Nation. Presumed dead following the events of The Last Jedi, she is revealed to have survived (albeit facially scarred) thanks to some Green Goblin equipment which appeared in her ship.
 * Appearance: The same as in The Force Awakens and The Last Jedi, but with her helmet replaced for a Goblin mask and hood.
 * DN-0001 (voiced by Dee Bradley Baker) - Phasma's second-in-command in the Dark Nation.
 * Appearance: Sporting a black and red variant of Adrian Toomes' Goblin King suit from the original Season 2' "Goblin War" arc. His real face is never revealed.
 * Bala-Tik (voiced by Jason Spisak) - The leader of the Guavian Death Gang who previously allied with the First Order for his vendetta against Han Solo, and is now a member of the Dark Nation.
 * Appearance: The same as in The Force Awakens, added with a red stripped black bodysuit and a Goblin mask and hood.
 * Sarco Plank the Scavenger (voiced by Ike Amadi) - A Melitto scavenger, arms dealer, and bounty hunter who once fought Luke Skywalker in the Temple of Eedit during the Galactic Empire Era. A member of the Dark Nation, he maintains his unique original arsenal and uses one of the group's Goblin gliders.
 * Appearance: The same as the 2015 junior novel The Weapon of a Jedi: A Luke Skywalker Adventure.
 * Unkar Plutt (voiced by Dee Bradley Baker) - A former Junk parts dealer on Jakku, he joined Phasma's Dark Nation in exchange of resources for revenge on Rey and her allies for the incident on Jakku, where Rey, Finn and BB-8 stole the Millenium Falcon from him. He is shown having been given a cyborg arm after his original one was ripped off by Chewbacca in Takodana (as shown in a deleted scene in The Force Awakens).
 * Appearance: The same as in The Force Awakens, and sporting a cyborg arm and a red stripped black bodysuit.

Decepticons
The Autobots' evil counterparts and main enemies, and a faction of villainous Cybertronian Transformers who seek to restore Cybertron by terraforming Earth.


 * Megatron (voiced by Fred Tatasciore) - The tyrannical leader of the Decepticons and captain of the warship Nemesis. He transforms into a silver and purple Cybertronian jet, which is based on his vehicle form in the first live-action Transformers film.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * Airachnid (voiced by Mary Elizabeth McGlynn) - A devious female Decepticon who transforms into a black helicopter resembling an RAH-66 Comanche, though while she is in robot mode, the helicopter's rotors can unfold and extend into long legs akin to those of a spider. She can also shoot spider webs from her wrists, produce a highly corrosive acid, and telepathically control the Insecticons.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.
 * Barricade (voiced by Keith David) - A former Decepticon scout who was promoted as second-in-command, he transforms into a black police car resembling his live-action Transformers film counterpart.
 * Appearance: The same as his IDW Publishing comic book counterpart with the colors of his 2007 film appearance, and animated after the characters in Marvel's Spider-Man.
 * Shockwave (voiced by Steve Blum) - An emotionless one-eyed Decepticon, Megatron's first lieutenant on scientific endeavors, and the creator of the Predacon clones, whose vehicle mode is a purple Cybertronian tank resembling his third live-action Transformers film counterpart.
 * Appearance: The same as in Transformers: Fall of Cybertron, and animated after the characters in Marvel's Spider-Man.
 * Soundwave (voiced by Ike Amadi) - An expert Decepticon and hacker who transforms into a blue UAV drone aircraft resembling an MQ-9 Reaper. While he is the most intellectual of the Decepticons, he speaks the least among them, and most occasionally quotes: "Soundwave superior. Autobots inferior.".
 * Appearance: The same as in Transformers: Fall of Cybertron, and animated after the characters in Marvel's Spider-Man.
 * Laserbeak - Soundwave's personal minion and a vulture-based Decepticon drone.
 * Appearance: The same as in Transformers: Fall of Cybertron, and animated after the characters in Marvel's Spider-Man.
 * Breakdown (voiced by John DiMaggio) - A powerful and brutish Decepticon warrior who transforms into a blue military truck resembling an Oshkosh Sandcat equipped with a missile turret on the roof.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man.

Individual

 * Dr. Ivo Robotnik (voiced by J. K. Simmons) - Sonic's arch-nemesis and a mad scientist and roboticist aiming to conquer Mobius with his inventions. He is the owner of Robotnik Industries, a high-tech company at San Francisco, although he occasionally faces allegations for controversially roboticizing living beings (including aliens he captured from Mobius).
 * Appearance: The same as in the 2006 Sonic the Hedgehog video game, and animated after the characters in Marvel's Spider-Man.
 * Starscream (voiced by Steve Blum) - Once Sky Commander of the Autobot Energon Seeker army back on Cybertron, Starscream was Megatron's second-in-command until, after taking over leadership on Megatron's brief absence, was banished and executed by his replacement Barricade. He was soon reanimated by Kree technology as a Predacon who can transform into a grey and red mechanical wyvern, and as Predaking's lieutenant.
 * Appearance: The same as in Transformers: Prime, and animated after the characters in Marvel's Spider-Man. His beast mode resembles the Egg Wyvern from the 2006 Sonic the Hedgehog video game.
 * Shredder (voiced by Lex Lang) - The Teenage Mutant Ninja Turtles' arch-nemesis, a fearsome samurai terrorist and the leader of the criminal organization known as the Foot Clan.
 * Appearance: The same as in Batman vs. Teenage Mutant Ninja Turtles, and animated after the characters in Marvel's Spider-Man.

Anti-Heroes

 * Venom / Eddie Brock (voiced by Ben Pronsky as Eddie Brock; Benjamin Diskin as Venom) - A former employee at the Daily Bugle and the current host of the V-252 / Venom symbiote. Initially a foe to Spider-Man, Brock and Venom soon became his allies after being swayed by Peter's memories of Uncle Ben into becoming a better person and hero.
 * Appearance: The same as in the original story.

Individual

 * Ben Parker (voiced by Patton Oswalt) - Peter's deceased uncle whose death inspired Peter into becoming Spider-Man. He occasionally appears in flashbacks of the story, including the one when Uncle Ben taught Peter that "With Great Power comes Great Responsibility". Peter would eventually carry on his uncle's knowledge for his current activities as a scientific genius and as a superhero, and with the equation: W / GP ⇒ GR serving as his greatest reminder.
 * Appearance: The same as in the original.
 * Richard and Mary Parker (voiced by Roger Craig Smith and Laura Bailey respectively) - Peter's parents (Richard is Uncle Ben's brother and Mary is Aunt May's sister) who were killed by Red Skull after Peter was left to the care of Uncle Ben and Aunt May and their younger daughter Teresa was left to the care of renegade Kree soldier Mar-Vell.
 * Appearance: Visually older versions of their children respectively.
 * TBD (voiced by TBD) - TBD
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 * TBD (voiced by TBD) - TBD
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 * TBD (voiced by TBD) - TBD
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 * TBD (voiced by TBD) - TBD
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 * Torbert Octavius (voiced by Charlie Adler) - Otto's abusive father, who disapproved of his son's love for science, saying that it was useless, and did not show much of a care that Otto was being picked on by bullies. He appears in "An Eye in the Sky", where he was shown watching TV until he was attacked by Otto (who had now become Doctor Octopus) in revenge for his past mistreatment. The outcome is not shown, but it is later reported by Betty Brant that Otto killed Torbert off-screen.
 * Appearance: The same as in the original show's episode "A Troubled Mind".
 * Flash Thompson (voiced by Benjamin Diskin) - A student jock at Midtown High and Osborn Academy. First appearing in "Osborn Academy", he initially picks on Peter and admires Spider-Man. Flash has traumatic encounters with the Venom symbiote in "Venom" and "On the Tiger's Claws", but gets rescued by Spider-Man both times. He was also an acquaintance to Mary Jane until she became resentful of him after he indirectly insulted her and other costumed party entertainers as "useless clowns" (a reason why Mary Jane dropped out of Midtown High to join Horizon High), and he has a estranged relationship with his two younger brothers Marcus (due to Flash disapproving Marcus' passion for entertaining children in his cartoon lion costume), and Jack (who views Flash as a loathsome bully due to the way he treats nerds and other jocks; it is also stated that Flash also used to mock Jack over his friendship with Cassie, which is a personal reason why Gwen is also resentful and distrustful of Flash).
 * Appearance: The same as in the original.

Main Episodes
While the first five episodes remain the same as in the original Season 2 plot, the story goes on with a new different storyline:

06) “On the Tiger’s Claws”
As Peter Parker enjoys a date with his girlfriend Mary Jane Watson (who had dropped out of Midtown High to enroll at Horizon High following a conflict between Flash Thompson and one of the football jocks), J. Jonah Jameson (ambitious for knowledge of how Peter obtains so much good pictures of Spider-Man) hires Scorpion to intercept Peter and interrogate him. His plans, however, are put in jeopardy when Eddie Brock (angry and upset over being recently fired from the Daily Bugle) is pushed to his breaking point, forcing him to get pictures for the V-252 at the Space Administration, leading him to be transformed into a new version of Venom. Having predicted the issuing chaos to happen, Spider-Man and Mary Jane (who happens to be the half-human, half-alien werecat Tigra) work on defeating the two villains while Amon and Jade (two young Skrull scouts disguised as the couple in General Talos’ behalf) gather the evidences of Jameson’s crimes to expose him.
 * Note: It is revealed in this episode that all of Peter’s friends (from both Midtown High and Horizon High) as well as Max Modell and Aunt May are now aware of his secret as Spider-Man since the events of "Between Ock and a Hard Place".
 * Outcomes: J. Jonah Jameson, exposed for hiring Scorpion to chase both Peter Parker and Spider-Man, is removed from the Daily Bugle and arrested for life. Eddie Brock is taken in to be punished by the Titanian Tiger tribe, and the V-252 is confiscated by the Skrulls to be lobotomized and stripped off its link from Peter. Meanwhile, Anya Corazon finds herself crawling walls like Spider-Man does, discovering in joy that she still has her spider powers from the Man-Spider incident.

07) “City of Heroes, Part 1: Spider-Gwen Returns”
Following J. Jonah Jameson’s arrest after the defeats of Scorpion and Venom, Peter is with Gwen Stacy overseeing a relic called the Blood Gem, which is said to contain mystical energies, after it was delivered to Horizon High after being found at an ancient dig site. Just then, the school is attacked by Electro, who is out to steal the Blood Gem on Silvermane’s behalf. During the chaos, Gwen exposes herself to the Blood Gem’s energies, which reawaken her dormant spider-powers, leading her to adopt the alias of Ghost-Spider as she and Spider-Man work to stop Electro and Silvermane from obtaining the Blood Gem. During which, Anya Corazon (having discovered that she still has her spider-powers from the Man-Spider incident) uses them to become Spider-Girl, as she and Miles Morales / Kid Arachnid work together in taking down Hydra spy Yelena Belova the Crimson Widow, who was sent by Arnim Zola to obtain the Blood Gem by any means necessary.

08) “City of Heroes, Part 2: Where There’s Smoke”
Caught by the radiations of a prototype Blood Gem after surviving an attack by Molten Man, Liz Allan earns the abilities to fly and manipulate and generate fire. Initially terrified of her mutation, Liz is still inspired by Spider-Man into becoming a new superheroine herself on the name of Firestar. As Spider-Man and Firestar work together to stop Mysterio from obtaining Oscorp technology to take over its newly developed drone units, Kingpin has his henchmen hunt for Screwball after she pulls a prank on his henchmen which costs him their researches for the Neuro-Cortex as she prepares for "the Ultimate Prank before her retirement". Little are both Kingpin and Mysterio expecting, however, a incredible surprise Spider-Man, Firestar and Screwball have been preparing for the two villains.
 * Note: It is revealed in the end that Screwball is still Liz wearing her suit above her Firestar outfit as part of her "Ultimate Prank".

09) “City of Heroes, Part 3: American Cats”
Spider-Man, Tigra and Kid Arachnid work alongside Kevin Hunt / American Knight and T’Challa / Black Panther to apprehend Vulture, who carries information about Killmonger and his plans with the Shadow Council. During which, they also cross paths with Flash Thompson's estranged younger brother Marcus (the feline vigilante known as the Lion King), who is out to stop Jack O'Lantern from getting revenge on Flash by targeting the students of Midtown High School. Things get harder when Killmonger sends Vulture after Jack O'Lantern and brainwash him as a member of the Shadow Council.
 * Outcome: Spider-Man is given by Black Panther a Vibranium particle enhancer which upgrades his web-suit, giving it a new look which now resembles the suit worn by Tom Holland's character in Captain America: Civil War and Spider-Man: Homecoming.

10) “City of Heroes, Part 4: More Than Human”
After overhearing the story of the peace treaty between Mutants and Inhumans, Spider-Man is informed of the existence of Kit Renner the Werefox, an orphaned child girl who happens to be a Mutant/Inhuman hybrid, and seeks to protect her by any means to preserve the peace treaty. He earns help from young Inhumans Ms. Marvel and Inferno and X-Men members Iceman and Rogue when it comes to protect Kit from Maximus the Mad and Magneto; both seeking to terminate Kit out of their protest of the peace treaty between their both kinds, and former military colonel-turned-fugitive William Stryker, who seeks to use Kit as a weapon against Mutants and Inhumans using new tech devices provided for him by the Master Planner.
 * Note: Ms. Marvel is revealed in this episode to be a trainee of the Avengers, not officially a member.
 * Outcome: Following Stryker's defeat by Spider-Man's hands, Spider-Man is honored for preservation of Mutant and Inhuman rights.

11) “City of Heroes, Part 5: Spider in the Eagle’s Shadow”
When Mister Negative and his Inner Demons arrive in New York seeking to take over the Ten Rings, forbidden relics which will unleash the Mandarin if in wrong hands, Spider-Man teams up with Lin Lie the Sword Master, Amadeus Cho / Brawn ant the Avengers (Captain America, Black Widow, Iron Man, and Hawkeye) to stop them. Meanwhile, Gwen, Miles, Anya and Kevin, all wanting to learn Spider-Man’s recently seen movements on Martial Arts following his fight against Chameleon, seek out his secret combat teacher Shang-Chi, who teaches the four more about the ancient arts of Kung Fu. During which, Spider-Man finds himself closer to figure out the identity of the mysterious Master Planner after the villain hires Taskmaster and his mercenaries to obtain the Ten Rings before both the heroes and Mister Negative do.
 * Note: The title of the episode is a reference to Snake in the Eagle's Shadow, a 1978 Hong Kong martial arts action comedy film directed by Yuen Woo-ping in his directorial debut, and starring Jackie Chan.

12) “Brainstorm”
As his friends (joined by Sam Alexander / Nova) resume to protect the citizens of New York and stop the recent riots in the city's criminal underworld, Spider-Man is called by Yuri Watanabe to help solve a mystery in the Supervillain Holding Facility, where both guards and inmates have gone berserk and are fighting each other until the facility collapses. Spider-Man finds out that the consciousness of Otto Octavius (the mastermind behind the recent chaos only identified to as the "Master Planner") has inhabited the body of the Living Brain, a robot made from the Neuro Cortex technology in the facility, and using the energy-focal device he made at Oscorp to begin making his enemies suffer. Octavius believes himself more than just prepared to fulfill his ultimate goal, but Spider-Man (who had predicted Octavius’ plans thanks to his lessons with Shang-Chi) still has a surprise element which Octavius was not prepared for.
 * Outcomes: Despite close to succeed, Otto Octavius' plan to swap bodies with Spider-Man backfires when Spider-Man (whose suit is shielded by a Vibranium particle enhancer provided for him by Black Panther which protects his brain from even the most powerful psychic attacks) redirects the Neuro-Cortex into the Living Brain and transports Octavius' consciousness into the energy-focal device. Later after returning Octavius back to his inactive body and delivering the Neuro-Cortex resources to his friend and new Horizon High classmate Carlie Cooper, Peter begins experiencing brief visions of his past, including one with his parents Richard and Mary Parker, on which he decides that his true work has just begun.

13) “What Girls Are Made Of”
While babysitting Gwen's little cousin Cassie Lang, Carlie Cooper tells her three stories about the amazing team ups between the super-heroines who fought alongside Spider-Man, which surprisingly happen at the same time outside of where they are. This episode features an anthology format split between three different segments:
 * 1) “Elements of Surprise” - Liz Allan / Firestar teams up with her best friend Michelle Jones, the Water-bending superheroine Wave, to stop a new group of criminals organized by Sandgirl in a warehouse of Oscorp.
 * 2) “Feline Pryde” - Mary Jane Watson / Tigra and her Mutant cousin Kitty Pryde / Shadowcat chase after Mystique, who has infiltrated A.I.M. as a double agent for Magneto to steal resources for the creation of new Sentinels.
 * 3) “Web of Shadows” - Ghost-Spider and Spider-Girl team up with Silk, an Inhuman with spider powers, to stop Madame Hydra, who had just ingested a serum based on Raymond Warren's Jackal formula and became the monstrous Viper and plans to collect the Jackal's mind-control researches, which she plans to use against Inhumans.
 * Note: Because this episode features female-centered storylines, it is therefore the only episode where Peter Parker / Spider-Man does not physically appear, despite being mentioned.

14) “The Parker Errand” (1)
After defeating the Living Brain and returning Otto Octavius’ consciousness back to his inactive body, Peter (having experienced surprising visions after his last exposure to the Neuro-Cortex) begins to solve secrets about his family’s past and find answers about what happened after his parents Richard and Mary Parker were killed by the Red Skull. His quest leads him to Gwen's grandfather Doctor Henry Pym, with whom Peter’s parents previously worked on secret projects of which the Red Skull and his followers have been unaware of. As Peter searches for these projects’ files and works alongside Mockingbird and She-Hulk to intercept Truman Marsh, who carries evidence of J. Jonah Jameson's past role in the Parkers' tragedy, and his A.I.M. units and stop them from obtaining the Tesseract (a alien relic containing the Space Infinity Stone) and use it to gain access to and invade Pegasus Z under their new leader MODOK's orders, Eddie Brock resurfaces with Venom bonded to him once again and, after experiencing memories of Peter's life (which include Peter's former teacher in Midtown High and Brock's lover Anne Weying) which sway them into being heroes, the two decide to stop A.I.M. in their own after discovering that Carlton Drake (the man who ruined Brock's life in San Francisco and ordered his employees to bring the Symbiote from its planet to Earth) also has ties with Marsh and MODOK.
 * Note: The plot is set during the events of previous episode.

15) “An Eye in the Sky” (2)
Now in possession of the Parker Projects' files and having decided to finish what his parents started, Peter begins to continue their unfinished projects, starting with AC-5 (or "Ace" as Gwen calls him), a very special drone powered by crystals of unknown origins. His work draws the attention of Inhuman king Black Bolt and his queen Medusa, who are familiar with the Parker Projects and offer to help Peter in exchange of his help with their ordeals with Ronan the Accuser, who seeks to collect a formidable relic from a Inhuman city to begin the Inhumans' extinction. Peter (as Spider-Man) eventually accepts their offer after learning that Ronan is also using Doctor Octopus' technology as a beacon for the Kree Empire to reach Inhumans on Earth and works alongside Silk, Inferno and bonded couple Cloak and Dagger to defeat Ronan and MODOK, who seeks the Kree Empire's technology to fully recover from his previous defeat. Meanwhile, a recovered Otto Octavius (incarcerated in the Supervillain Holding Facility) has trouble remembering the codifications for the Neuro-Cortex and riding himself from his own repressed memories from the past, including issues with his own abusive father Torbert and bully jock Steve. Consequently, Octavius breaks out of his cell, reclaims his tentacle harness and rushes to retake the Neuro-Cortex from Max Modell, but will be facing more memories of his past failures when he comes across Carlie Cooper, who is enrolling at Horizon High as a new student.

16) “The Calm Before the Stormranger” (3)
As he moves on to his parents' second project, which involves his father's old A.I. assistant J.A.R.V.I.S. and a robot body hidden in the underground of Horizon High, Peter (as Spider-Man) battles a cabal of bounty hunters hired by Athena Gonzales of Athena Industries to destroy Stark Expo and the Central F.E.A.S.T. shelter as he gains a new ally with Stormranger, a mysterious armored female individual who has been protecting the F.E.A.S.T. shelters from any supervillain threat in the city. Coming close to learn of Gonzales' past grudges with his parents, Peter will soon learn that there are more amazing truths about Stormranger than he can believe. Meanwhile, Gwen and Anya play a helping hand about the robot body for Jarvis, and (through its special tracking monitors) they discover that there is one more living Parker in New York besides Peter himself and Aunt May, leading them to believe that Peter has a long lost sibling.
 * Outcomes: After analyzing Stormranger's biological DNA, Ace and Jarvis reveal to Peter that Stormranger shares his same biological DNA blood sample, making Peter realize that Stormranger is also his parents' daughter, and his long lost younger sister.

17) “Sons of Fathers” (4)
As his friends help him with his parents' third project, which involves a giant alien robot body (code-named "Arsenal") requiring a human consciousness to host, Peter works to reconnect with Stormranger (who was revealed by Ace and Jarvis to be none other than Richard and Mary Parker's second-born child and Peter's long lost younger sister), whereas Stormranger seeks to bring down a Oscorp facility controlled by Donald Menken, who seeks to sell Oscorp's technology for Hydra, and Ant-Man chases Yellowjacket, who seeks to take over Oscorp's technology for his own purposes. Harry Osborn (back to his mantle of Hobgoblin) and Venom (who is chasing a Symbiote spawn which possessed one of the Hydra agents) also join Peter in his fight, but Harry will be making heavy decisions after learning that his father Norman also played a shocking role in turning his best friend's parents over to the Red Skull.
 * Outcomes: Harry Osborn (disillusioned with his father's past actions and remorseful of the Parkers' tragedy) sacrifices himself to destroy the Oscorp facility as Menken (injected by Arsenal with a brain virus which forces him to permanently hallucinate himself in a dark world of Menken's worst nightmares) and Yellowjacket are arrested. After Harry's funeral, Stormranger (real name revealed as Teresa) still decides to reside in the K'un-Lun Temple with Shang-Chi until the persecutors of hers and Peter's parents are dealt with, while Peter is encouraged by his friends to finish his parents' projects in theirs and Harry's memory.

18) “Seeds of Life” (5)
Determined to honor Harry's sacrifice by bringing Norman (who is revealed to have survived his apparent death and donned a new Green Goblin armor) to justice, Peter (followed by Gwen, Ace, Jarvis and Arsenal (now the host of the consciousness of Carlie's father Sergeant Chris Cooper)) travels to a HYDRA base where Viper is overseeing the creation of new human clones to be part of Red Skull's army. As he seeks to complete his parents' fourth and final project, which involves a emphatic insectoid alien (whom Gwen names "Mantis") which has a symbiotic link to him, Peter and Gwen run into Black Panther, who was pursuing the Shadow Council's last remaining member Madame Masque after she managed to break Otto Octavius out of prison so he would aid her in her goals with Arnim Zola's technology, and the Avengers, who are on a mission of their own against Hydra. While the three villains consequently clash against each other for control over the base, Peter seeks out a inactive clone body for Mantis to complete her transformation as Stormranger (real name revealed as Teresa Parker) returns seeking to settle her ordeals with Osborn once and for all.
 * Outcomes:
 * 1) Mantis earns a new humanoid form by merging with the strongest of the clone bodies created by Viper.
 * 2) Red Skull is hit by Green Goblin's out-of-control glider and dragged into Madame Masque's ship, which is obliterated by Arsenal along with Red Skull himself. The progress also results in Madame Masque's mask getting destroyed, rendering her powerless and freeing Octavius from her influence.
 * 3) Norman Osborn is defeated by Spider-Man, Stormranger and the rest of their friends and exposed to public eyes by Ace.
 * 4) Freed from Madame Masque's influence, Otto Octavius is taken to a mental hospital until he recovers.
 * 5) Teresa (pleased to know her parents' persecutors have been brought to justice) decides to live a new happy life with her brother Peter and Aunt May.

19) “Brand New Champions”
With his parents' projects finished and their persecutors facing final justice for their crimes, Peter resumes his newly improved life as a Horizon High student and superhero while his sister Teresa (having decided to live with him and Aunt May) puts her new life on track as she enrolls at Midtown High School. The siblings and their friends (having formed the superhero group named the "Champions") soon find themselves against a new incarnation of the Sinister Six (consisting of Silver Sable, Electro, Alistair Smythe / Spider-Slayer, Molten Man, Scorpion and organized and led by the Jackal), who are intending to fill the power void left after Norman Osborn's arrest and the fall of Oscorp, whereas Vulture and the Wake Riders resurface seeking a weapon stolen from the Kree Empire for Oscorp which is now in possession of renegade Kree soldier Mar-Vell.

20) “Real Wild Initiation”
While his friends work on their new headquarters (which takes place in his parents’ former research center, the Triskelion), Spider-Man is entrusted to look after and train Marcus Thompson’s younger brother Jack and Gwen's cousin Cassie Lang, who had just become Speed and Ant-Girl respectively thanks to Kree relics which granted them similar powers and resources of Quicksilver and Ant-Man respectively. Meanwhile, Carolyn Trainer (encouraged by Otto Octavius' final defeat and Norman Osborn’s downfall) once again dons the moniker of Lady Octopus in the purpose of redeeming herself from her previous actions in Octavius’ favors. Spider-Man will soon have to team up with Speed, Ant-Girl and Lady Octopus when Madame Masque (now equipped with Octavius' tentacle harness and Osborn’s Green Goblin technology) resurfaces seeking the Neuro-Cortex to rebuild her destroyed ship and retain her full power, and Kree Empire operatives Yon-Rogg and Minn-Erva seek to retrieve the Kree energy extracted by the children by any means necessary.

21) “Legendary Universes, Part 1: Message from the Unknown”
Wandering around the Champions’ new headquarters, Peter and Carlie come across a dimensional portal device which Peter’s parents used to collect the resources for their scientific projects, and decide to analyse it. During which, Spider-Man finds himself dealing with the ambitions of J. Jonah Jameson's son John, who had donned a power suit of Alchemax's technology to become the self-proclaimed superhero Captain Ultra with the goal of clearing his father's name out of his role in the Parkers' tragedy (but also to make money with his popularity as Spider-Man acknowledges). Things get no better when Yuri Watanabe's former mentor Augustus Roman (also known as the Regent) emerges with a power-replicating suit also made of Alchemax tech and seeks to bring New York to his knees. Spider-Man begrudgingly teams up with Captain Ultra to take down Regent and stop him from reaching the portal device, which the Regent plans to use to collect the discarded Oscorp protocols which have gone missing in the dimensions which Peter's parents explored. During which, Tigra and Kid Arachnid discover that Alchemax's CEO Tiberius Stone is also in league with the Regent's madness and seek evidences to expose him.

22) “Legendary Universes, Part 2: Webs of the Force”
Certain that the Regent will come back from the Negative Zone (where Captain Ultra imprisoned him before Spider-Man could strip Regent off his suit's Power-absorbing functions), Spider-Man decides that he must travel into the dimensions his parents explored to seek and destroy the discarded Oscorp protocols before Regent can return to retrieve them. Followed by Stormranger, Firestar, Lin Lie, Nova and Ace, Spider-Man's first journey takes place in AC-5's home dimension: the universe of the Jedi Alliance. There, they team up with Rey Skywalker and her allies from the New Jedi Order and the Resistance to take down the Dark Nation (a cult-like organization composed of former members and loyalists of the First Order and led by Captain Phasma and equipped with Oscorp's Green Goblin technology), and save the Galaxy once more.
 * Crossover with the Star Wars franchise

23) “Legendary Universes, Part 3: Unlimited Speed”
Resuming his journey across dimensions to destroy the Oscorp resources to prevent Regent's rise to power, Spider-Man now travels alongside Tigra, American Knight, the Lion King, Wave and Jarvis into Jarvis's home dimension: the universe of Sonic the Hedgehog and the Mobian Freedom Fighters (consisting of Sonic and his friends Sally Acorn, Miles "Tails" Prower, Sticks the Badger, Nicole the Holo-Lynx and Knuckles the Echidna). There, the Champions and Freedom Fighters work together to destroy a prototype of Otto Octavius' tentacle harness which fell in possession of EggRobo, who seeks to take over Earth's Technology Center to roboticize humans and other organic life forms. The heroes must also contend with Dr. Robotnik, who seeks to regain control over EggRobo and retrieve Jarvis' robotic body by any means necessary.
 * Crossover with the Sonic the Hedgehog franchise

24) “Legendary Universes, Part 4: Heroes in Disguise”
Having reached halfway of his objective, Spider-Man now travels alongside Ms. Marvel, Ant-Girl, Speed, Inferno, Brawn and Arsenal into the home dimension of Arsenal's robot body: the universe of the Cybertronian Transformers. There, they team up with Optimus Prime and the Autobots to defeat the Predaking, who was resurrected by crystals powered by Kree technology which was salvaged and discarded by Oscorp's engineers weeks ago and now seeks to convert human technology to his own liking and take revenge on the Decepticons for attempting to enslave him. The heroes also have to contend with Megatron and his Deception followers, who wrongly accuse the humans for unleashing Predaking to the world and Cybertron.
 * Crossover with the Transformers franchise

25) “Legendary Universes, Part 5: Of Spiders and Turtles”
With only one step of his objective remaining, Spider-Man now travels alongside Ghost-Spider, Kid Arachnid, Spider-Girl and Mantis into the fourth and last dimension his parents explored: the universe of the Teenage Mutant Ninja Turtles. There, the Spider-Team and the Turtles team up to take down a clan of ninjas who were physically mutated by samples of the Jackal's animal formulas and are on a rampage across New York. Things may get more serious when the Shredder seeks to tame the mutated ninjas and convert them as hounds of the Foot Clan to bring New York to its knees.
 * Crossover with the Teenage Mutant Ninja Turtles franchise

26) “Legendary Universes, Part 6: Ultimate Alliance”
With the Oscorp resources destroyed, Spider-Man and the team find themselves ready to take down Regent, who returns from his imprisonment more powerful than he was before after his power suit absorbed Antimatter from the Negative Zone. Even though Regent manages to subdue and drain Captain Ultra, replicate Doctor Octopus' technology and bring a new army of his own from the Negative Zone to Earth, Spider-Man and the team are still not alone, as they are joined in the battle by their allies, including the Avengers and the heroes from the parallel dimensions (the Jedi Masters, the Freedom Fighters, the Autobots, the Teenage Mutant Ninja Turtles and their allies), to defeat the Regent's army and save the world.

Differences Between the Canon Season 2 and Webbed Champion
Although the first five episodes of Season 2 remain the same, Webbed Champion follows a different storyline, with different outcomes.
 * Despite still becoming Ghost-Spider and Spider-Girl respectively, Gwen Stacy and Anya Corazon remained unaware of Spider-Man's identity of Peter Parker during Season 1 and the canon Season 2. In Webbed Champion, they are eventually revealed to have learned during Spider-Man's battle with the Neuro-Cortex-powered Doctor Octopus in "Between Ock and a Hard Place" (as mentioned in "On the Tiger's Claws").
 * Additionally, Peter was unaware that Anya was operating as Spider-Girl in the original show, but was aware of this fact in Webbed Champion.
 * New characters appear in the show, such as Black Panther, Teresa Parker, Inferno, and more.
 * Mary Jane Watson, who had a minor cameo in the original show, now has a central role in the Season, appearing as the show's version of Tigra.
 * Liz Allan also has a expended role, on which she becomes a new version of Firestar.
 * Along with expanded/speaking roles in the show, Captain America and Captain Marvel's designs are altered; Captain America now uses his design from Avengers: Black Panther's Quest, while Captain Marvel now resembles her counterpart from Marvel Rising and sports the costume worn by Brie Larson's character in the MCU.
 * Spider-Man's battle with Eddie Brock / Venom is altered; in the canon show, Venom managed to kidnap the key figures of Peter's life (Gwen, Anya, Aunt May and Max Modell) and hold them hostage in Horizon High to draw Spider-Man out. In Webbed Champion, the battle is now consisting of Scorpion against both opponents, and including Tigra on Spider-Man's side.
 * Compared to the canon story, J. Jonah Jameson is less sympathetic and has a more antagonistic role in Webbed Champion, on which he hires Scorpion to intercept Peter for pictures of Spider-Man, and is revealed to have had a role in Richard and Mary Parker's death by covering their deaths at Red Skull's hands as a "tragic, inevitable accident" for protection money.
 * The "Bring on the Bad Guys" arc, where Otto Octavius (through the Living Brain) places a bounty on Spider-Man's head and sends numerous super-villains after him, is replaced for the "City of Heroes" arc, on which Peter's friends slowly become new superheroes and join his battles against crime, although Otto Octavius being on the Living Brain is still intact.
 * The Canon Season had the "Superior Spider-Man" arc (from "Living Brain" to "Superior"), where Doctor Octopus swapped bodies with Spider-Man to try to become better than him. In Webbed Champion, however, the arc is replaced for "Family Business" arc ("Brainstorm" - "Seeds of Life"), where Peter works to solve secrets about his family's past and finish the projects his deceased parents started.
 * Therefore, Doctor Octopus' plan (which succeeded in the canon story) fails in Webbed Champion.
 * Plus, Peter's relationship with Gwen, Anya, Modell and the Avengers is not damaged by Octavius like it was in the original story, nor does he leave Horizon High to re-enroll at Midtown High.
 * Additionally, Anna Maria Marconi never gets to interact with Otto Octavius like they did in the original story.
 * Despite their details and traits from the original show remaining the same, Cloak and Dagger's origins are altered. In the original show, they were subjects of Tiberius Stone's experiments in Alchemax. In Webbed Champion, they are depicted as a couple of Inhumans who currently reside in Attilan, and work closely for King Black Bolt and Queen Medusa.
 * The season's final arc consists of a crossover with the fictional franchises of Lucasfilm's Star Wars, Sega's Sonic the Hedgehog, Hasbro's Transformers and Mirage Comics' Teenage Mutant Ninja Turtles.
 * In the original show, Adrian Toomes / Vulture was the main antagonist in the final arc "Goblin War", on which he led the Goblin Nation (as the Goblin King) to attempt to destroy New York. In Webbed Champion, the final arc's main antagonist is Augustus Roman the Regent, who takes control of Jackal's new Sinister Six and seeks to establish a new order in New York City through iron fist.
 * Slyde's origins are also altered; in the original show, he was a former worker at Beemont Chemical Corporation who was fired by the company's CEO Alan Beemont for wanting credit for what he developed for the company. In Webbed Champion, Slyde is Alchemax's armored super-soldier and a collaborator of the Regent's campaign to bring new order to New York City.
 * While still a minor antagonist in the final arc, Alistair Smythe never enrolls at Horizon High in Peter's place.

Secret Champions
Secret Champions is a series of six animated videos released on the Marvel HQ YouTube channel. The shorts are between six and seven minutes long and are released weekly. Similar to Marvel Rising Ultimate Comics, the videos combine comic book-style art with digital motion effects. The shorts serve as a tie-in and prelude to the episodes in the show, revealing untold details of the events, such as Teresa Parker's principles as Stormranger, and Arsenal's acquisition of his disguise of a Quinjet for his Vehicle Mode. Stories of the Crossover universes explored by Spider-Man and the Champions in the "Legendary Universes" arc are also featured.
 * 1) “Lost Daughter” - Set between "School of Hard Knocks" and "On the Tiger's Claws", the origin story of Teresa Parker as Stormranger is revealed. From the moment she ran away from the orphanage she was left in after her parents' death, to the moment she came in contact with her nanosuit, adopting the alias of Stormranger.
 * 2) “Military Update” - Set between "Sons of Fathers" and "Seeds of Life", Chris Cooper (whose Neuro-Cortex-preserved consciousness now inhabits the body of giant robot Arsenal) wanders around New York while working to remain hidden from most of the citizens, although he ends up discovered by Ms. Marvel, who even so befriends him. The short also reveals how Arsenal acquired his vehicle form of a Quinjet.
 * 3) “The Force Reborn” - Set in the Star Wars universe and following the events of The Rise of Skywalker, Rey Skywalker arrives in Canto Bight and encounters Temiri Blagg, a Force-sensitive boy whom she trains in using the Force. She also helps him save his friends from Bargwill Tomder's grasp. Mandalorian bounty hunter Din Djarin also arrives there seeking to collect a new bounty placed on Tomder's head.
 * 4) “Speed Me Up” - Set in the Sonic the Hedgehog universe, Sonic and Tails work to secure Hope Kintobor, who is being targeted by a pack of EggRobo's drones to be roboticized. Meanwhile, Sally Acorn is in Mobius with Longclaw, who teaches her and Sticks the Badger some secret features within the Mobian Rings. This short also reveals the moment Sonic's human friends Tom Wachowski and his wife Maddie adopted Hope as their own daughter, and explains how Sonic earned brief, but strong knowledge about life in Spider-Man's dimension.
 * 5) “More Than Meets the Eye” - Set in the Transformers universe, Bumblebee, Arcee and Grimlock are followed by Izabella Morgan in a mission to rescue Slug from Starscream, who has been ordered by Predaking to abduct Slug so they can convert him into a Predacon. This short also explores more details about the Seekers, the Cybertronians from which Arsenal's robot body originated before it fell in the Parkers' possession for experimentation.
 * 6) “The Mutagen Condition” - Set in the Teenage Mutant Ninja Turtles universe, Leonardo and his brothers team up with Casey Jones in saving April O'Neil from being converted by the Spider Emperor as a member of his clan of Super-Animals. This short explores some more of the origins of the Spider Emperor, which also include his past as a sworn enemy of the first generation of the Foot Clan.