The Amazing Spider-Man (Disney+)

The Amazing Spider-Man is an American animated series based on the Marvel Comics superhero, Spider-Man. The series is for Netflix and produced by Marvel Animation. The show centers around Peter Parker uncovering the mysteries behind the presumed deaths of his late parents, Richard and Mary Parker.

Over the course of the series Peter's friends and classmates gain spider powers of their own; Gwen Stacey as Ghost-Spider, Miles Morales as Kid Arachnid, Eddie Brock as Venom and Cindy Moon as Silk. Together they form a spider-themed team, the Web Warriors while his best friend Harry Osborn eventually becomes Neo Goblin. The Show features the appearances from various well-known Marvel superheroes; including the Avengers, the X-Men, the Fantastic Four, Moon Knight, Luke Cage, Daredevil, Elektra and Doctor Strange

The series' character designs and art styles are based off the early first season promotional artwork for Marvel's Spider-Man (2017) by Patrick Brown. This article is under the ownership of Dino-drones!

Synopsis
Peter Parker was an average high school student in New York City with a keen scientific mind that was bitten by a genetically enhanced spider during on a school fieldtrip to Oscorp Labs and was given amazing superhuman-like spider powers. After tragically losing his beloved Uncle Ben at the hands of a burglar, Peter then decided to uses both his powers and talents for good by becoming a superhero, the Amazing Spider-Man to honor the legacy of his Uncle Ben with his famous motto and lesson "With great power, there must also come great responsibility". Spider-Man will face many formidable supervillains in the criminal underworld, including The Kingpin, the Green Goblin, Doctor Octopus, Venom, Shocker & Clash, Rhino, Electro, Scorpion, Vulture, Sandman, the Lizard, Mysterio, Kraven the Hunter, Carnage, Stunner, Vermin, Mr. Negative and many more.

In addition, Peter embarks on a mission to uncover the mystery of genetic spiders origins at Oscorp, finding what really happened to his parents, Mary and Richard Parker who supposedly died in plane crash 12 years ago and uncover the secrets behind the Gentleman, Gustav Fiers.

Season 1
The show begins with Peter being enrolled at Horizon Academy, a futuristic high school owned by it's headmaster, Max Modell. While being Harassed and teased by Flash Thompson and his popular posse, Peter is close with his classmates and friends Gwen Stacy, Harry Osborn and Mile Morales. While Spider-Man continuously stopping petty crimes, he unknowingly gains the attention of the Kingpin, the feared rumored crime lord who organizes and oversees crimes throughout New York City.

Main Characters

 * Spider-Man/Peter Parker (Robbie Daymond): The main protagonist of the series. A 16-yr old high school student who gets bitten by a experimental enhanced spider on a school field trip to Oscorp Labs a year ago. While reeling from the effects of the spider bite, Peter discovered he had developed amazing spider-like superhuman abilities. While examining his new powers, with the resources from his house, Peter created a costume and goes to a wrestling venue, using the stage name "the Spider" to test his powers in a steel cage match to win prize money. After the match, a thief robbed the manager who cheated Peter from his prize money so he let the man run passed him and escaped in the elevator, nodding it wasn't his problem. Peter then returned home and saw police cars outside the house. He rushed inside to see what's going on, he sees Captain Stacey with aunt May crying on the couch. Stacy informed him that a burglar broke into the house demanding money and asks keys to the scar. His uncle Ben tried to stop him off guard but guy was armed. Infuriated, Peter then suit to find the man at an abandoned warehouse at the docks surrounded by the police. Peter then captured and unmasked him, only to discover in horror that it was the same crook he let get away at the venue. He left the man tied in webbing and was founded by the police. To honor the memory of his late uncle Ben, Peter sets out to use his powers for good to help those in need, wearing a new refined skin-tight body suit, designed a pair of web shooters and taking up the superhero alias, the amazing Spider-Man to protect the citizens of New York City. Peter works as a freelance photographer at the Daily Bugle for his boss, J. Jonah Jameson and an internship at the ESU lab with Dr. Curt Connors, alongside his friends, Gwen and Debra. Several months his superhero career, Peter has become an experienced and masterful crime fighter. Spider-Man's prominent trait is his sense of humor, he gained more confidence in himself and more talkative while making jokes in during dangerous situations. And is known to taunt, distract, insult and agitate his adversaries, or just to have fun. Peter Parker has a lean yet muscular and well defined build standing over 5'7" in height. He has brown eyes and brown hair. His spider suit's primary colors being red and blue, with a black silhouette of a spider on the chest area and a large red spider on the back. The red section has a black pattern that resembles that of a spider web, with the center of the web being on the mask between the eyes and on top of the nose. The expressive eye lenses on the mask are white with a bold black outline around them. His wrists, where his web shooters reside underneath and shoe welts are blue.
 * Mary Jane Watson (Ali Hillis): Spider-Man's main love interest, neighbor and Anna Watson's niece. A young 16-yr old high school student and Peter's classmate at Horizon Academy. Mary Jane was on the field trip to Oscorp Labs. She is a skilled actor and has a passion for the theatre, and pursues her dream throughout the series. She is also talented with martial arts for as she said 'Self defense classes are a must for a single girl'. Mary Jane has a slender and feminine build with lime green eyes and wavy Scarlett red hair that reaches her mid-back.
 * Harry Osborn/New Goblin (A.J. LoCascio): The childhood best friend of Peter Parker and Gwen Stacy and the son of Norman Osborn. Harry had always a strained relationship with his father, who had expected high expectations for his son but was often too busy to be there for him and constantly lives in his father's shadow. Never the less, Harry's mother Emily had always been there for her son whenever he's lonely. He has a strong friendship with Peter Parker. Harry has auburn hair and olive eyes
 * Gwen Stacy/Ghost-Spider (Ashley Johnson): Peter and Harry's best friend and daughter of Police Capt. George Stacy. Gwen is a science whiz at Horizon Academy and a skilled drummer. Gwen has turquoise eyes and long blond hair w/h face framing bangs and tips dyed in pink. Gwen's Ghost-Spider costume is a hooded form-fitting black bodysuit leaving the torso, cowl and arms white. The eyes on the mask are white with a bold pink outline. The interior of the hood, biceps and under arms is neon pink with cyan web patterns and the shoe welts are also cyan.
 * Miles Morales/Kid Arachnid (Nadji Jeter): Miles is a 14-yr old student at Horizon Academy and becomes another Peter's closets friends in Season 2. His parents are Rio Morales, who is a nurse and his father is Jefferson Davis, who is a police officer. Miles eventually gets bitten by a different genetically enhanced spider dubbed "Electrolis Arachnatis" and gives him spider powers but with bio-electrical traits, he can discharges a powerful red electrical Venom Blast, capable of paralyzing most foes with a mere touch. and the ability to camouflage himself to the surroundings. Miles wears his traditional Ultimate Spider-Man suit.
 * Venom/Eddie Brock (Matt Lanter): Eddie Brock is a journalist and rival photographer of Peter Parker at the Daily Bugle until he was fired by Jameson for using faked photos of Spider-Man robbing a bank. Disgraced and humiliated by his actions, Eddie Brock became resentful and expressed his hatred for both Spider-Man & Peter Parker for ruining his career, reputation and getting kicked out of ESU. Overwhelmed by shame and humiliation, Eddie goes to the Lady of Saints Church to pray for vengeance on Spider-Man for ruining his life. After the black symbiote was rejected by Spider-Man, weakened the sounds of the church bell, it stumbles upon Eddie and sensed his hatred and animosity for Spider-Man and Peter Parker. The symbiote then subsequently latches and bonds with him and revealed him Spider-Man's duel identity. The union of Brock and the symbiote resulted in the creation of a monstrous, bulky-framed creature resembling black-suited Spider-Man, but with a frightening fang-filled elongated mouth and a long prehensile tongue. And thus, the symbiote super-brute Venom was born. The symbiote increases Brock to superhuman levels with strengths and abilities that are stronger Spider-Man's but can produce andshoot symbiote webbing from his wrists stronger than Spidey's web fluid for web swinging and it makes him undetectable to his spider sense. Venom can also uses his own symbiotic tendrils to ensnare objects and for combat.
 * Cindy Moon/Silk (Vyvan Pham): A 16-yr old Korean-American student at Horizon Academy who later becomes another friend of Spider-Man. It's revealed that Cindy was bitten by the same spider that granted Peter Parker his amazing abilities. As her powers manifested, Cindy's parents were approached by a man named Ezekiel Sims, who offered to train their daughter in her newfound gifts. Though he helped her for a time, Ezekiel eventually locked Cindy away in a bunker to "protect" her from those who would use her gift for evil. Cindy spent a decade in this bunker before she was founded and freed by Spider-Man. After she was freed, Peter revealed is secret identity to her. This event changed her life, leading to her becoming the superheroine known as Silk with training from Spider-Man, Kid Arachnid and Ghost Spider and it would begin her quest to find her missing parents, who disappeared during her time in the bunker. Cindy also became an intern for volunteer work at F.E.A.S.T. thanks to the recommendation of her friends Peter Parker and Gwen. She has the same superhuman spider abilities as Peter but can shoot organic webbing through her under wrists and fingertips. Her spider costume is a formfitting blurple bodysuit with a white torso, a red spider web design across the chest, with a red 'S' symbol at the center, and an indigo half mask covering her mouth, red gloves (only her thumb, index and middle fingers), and the tip of her shoes are white.
 * Teresa Parker (Stephanie Panisello): Teresa is the long lost younger sister of Peter Parker and the daughter of Richard and Mary Parker. When she was born, her parents left in the care of the foster home then years they were killed by thugs at a super market when she was 12. Nick Fury took her in and got her into the CIA for a brief time for Teresa to get information on her birth parents and the whereabouts of her brother, Peter.
 * Gustav Fiers/The Gentleman (Richard Doyle): The central antagonist of the series. An mysterious assailant who knows Peter's secret identity and has a history about his late parents Richard and Mary Parker. He keeps to the shadows in the background overseeing Spider-Man's activities. Gustav is an old associate of Hydra. In this series, The Gentleman is involved in the creations of various supervillains such as: he who caused the accident that turned Max Dillon into Electro, sent the enhanced Super Soldier Serum to Angelina Brancale that led her to become Stunner and provided Roger Goshaw the Multi-Man armor.

Recurring/Supporting Characters

 * Aunt May (Minnie Driver): Peter's loving aunt and the widowed wife of Benjamin Parker. May has been taking good care of Peter with her husband after Richard and Mary left their son in their care. She is as young and flexible as her MCU counterpart, and capable with self defense in her mid 50s. She is also eccentric as her Ultimate counterpart is, much to may's shock and Peter's embarrassment.
 * Uncle Ben (Paul Eiding): Peter Parker's deceased Uncle and beloved husband of May Parker. When Richard and Mary left their son in his and May's care, Ben did everything he could to make Peter turn out alright and has encourage him into the pursuit science. One fateful night, Ben died while trying to stop a burglar who broke into their house demanding him and May money and the keys to his car. After his death and funeral, Peter then decides to become a superhero to honor his memory by the lesson he taught him: "With great power, comes great responsibility". Shown on flashbacks such as during Peter's dilemmas. Ben told his nephew that his father knew that his son would do great things.
 * Richard Parker (Neal McDonough): The father of Peter Parker and Ben Parker's younger brother who was a brilliant scientist working at Oscorp in bio engineering studies for Bio Species Genetics. During his employment at Oscorp, Richard Parker had been work on a research project that involved with genetically enhanced spiders that could be used to create a vaccine for illness and diseases. However, Norman had plans to weaponize Richard's research and create biological weapons and sell them to powerful organizations like Hydra. After Richard discovered Norman's intensions, he terminated the project and left Oscorp later became a SHIELD agent after being recruited by Col. Nick Fury and joined SHIELD's research division. Richard resumed on his research in secret and soon met a beautiful agent, Mary Fitzpatrick. Over the couple of months of going on missions, two fell madly in love with each other and eventually they became married and had a son they named Peter. Richard has brown hair and brown eyes much like his son.
 * Mary Parker (Danielle Bisutti): The loving mother of Peter Parker and Richard's beloved wife. She was decorated SHIELD agent before meeting her husband-to be. They have been on multiple missions and situations past years of working together. Mary grew interested in Richard's research to help people. When Mary was pregnant, She and Richard took leave of absence from SHIELD and gave birth to their baby boy, Peter and later a daughter, Teresa. Mary Parker is a highly skilled and experienced field agent trained in martial arts and hand to hand combat tactics, espionage, firearms and has great athleticism. Mary has long reddish brown hair and hazel eyes. She usual wears her SHIELD jumpsuit.
 * Max Modell (Christopher Swindle): The headmaster of Horizon Academy and a mentor to Peter, Gwen, Miles and Cindy. Peter knew Max when he was a kid at Horizon Science Camp.
 * Flash Thompson (Jack DeSena): Flash is a jock at Horizon Academy who often bullies Peter Parker and his friends along with Kenny, and Jason. Flash is the quarterback of the Horizon Academy football team, the Horizon Crash-Drives. He's also a huge fan of Spider-Man.
 * Liz Allen (Tia Texada): A cheerleader at Horizon Academy and ex-girlfriend of Flash Thompson. She became Harry Osborn's eventually girlfriend. . She wars an outfit similar to her Spider-Man 2017 film counterpart.
 * Sha Shan Nguyen (Rino Hoshiro): A student at Horizon Academy and Flash's new girlfriend.
 * Randy Robertson (Khary Payton): A High school jock member of the Horizon Academy Football team and Robbie Robertson's son.
 * Kenny "King Kong" McFarlan (Matt L. Jones): A Horizon Academy school jock who is Flash's obese yet strong best friend. Kenny is also a member of the Midtown Football team with Flash, Randy and Jason.
 * Glory Grant (Dana Davis): A student and a cheerleader at Horizon Academy who is Randy's girlfriend.
 * Sally Avril (Holly Fields): A cheerleader at Horizon and Liz' friend.
 * Jason Ionello (A.J. Buckley): A Horizon Academy jock and One of Flash's friends with a slender build.
 * Brian "Tiny" McKeever (Jeff Bennett): A Horizon Academy jock and a member of the football team.
 * Angela Golden/Golddigger (Emmanuelle Chriqui): The well-bread daughter of wealthy businessman Matthew Gilden and the a love interest to Spider-Man. Angela is a well enrolls to Horizon Academy has become a very popular student. Angela serves the rival to Mary-Jane for the affection for Peter Parker. Angela is very beautiful and attractive young woman with an athletic body amber eyes and flowing blonde hair reach down to her lower back, toned broad shoulders, buxom breasts, long legs, and pick lipstick. She wears a black forming fitting bodysuit merged with tights, which leaves her shoulders and arms bare while wearing long sleeve metallic gold Bolero Shrug top.
 * Jenny Carson :
 * Seymour O'Reilly :
 * Captain George Stacey (Jon Schneider): Gwen's father and a leading member of the NYPD who believes Spider-Man is a true hero to the city, albeit working outside the law, defending him when he is seen as otherwise, saving his daughter from time to time. Even when Spider-Man was being framed, George always give him the benefit of the doubt, justifying his actions and often requests his help. Captain Stacey acts as a foil to the opposition of J. Jonah Jameson. George knew Richard Parker from college and He and his wife Helen were present at Richard ad Mary's wedding ceremony.
 * Helen Stacy (Nancy Sullivan): The wife of George Stacy and Gwen's mother.
 * Rio Morales (Jacqueline Pinol): Mile Morales's noble Hispanic, hospital nurse and eccentric mother
 * Lieutenant Jefferson Davis (Phil Morris): Mile's father who is a respected police officer of the NYPD. Jeff is also seen as a good friend to George Stacy.
 * Felicia Hardy/Black Cat (Audrey Wasilewski): Black Cat is an infamous vigilante and professional thief who is a secondary love interest of Spider-Man.
 * Daily Bugle Staff
 * J. Jonah Jameson (J. K. Simmons): The Editor-in-chief/Publisher of the Daily Bugle that notoriously slanders and gives low opinions about Spider-Man for being a masked menace to the city. Jameson is stereotypical newspaper editor who is gruff, impatient, cynical, arrogant, and stubborn to work with. Despite this, deep down Jameson is good man who cares for everyone who works for him and an honest ethical journalist that is dedicated to the truth.
 * Joseph "Robbie" Robertson (Gary Anthony Williams): The newspaper editor at the Daily Bugle and JJ's right hand man who often opposes Jameson's opinions on Spider-Man, seeing him as a hero. He is the father Randy Robertson.
 * Betty Brant (Rachel Kimsey) JJ's personal secretary at the Daily Bugle.
 * Ned Leeds (Eric Bauza): A reporter
 * Frederick Foswell/Patch (Keith Silverstien): An undercover reporter for the Daily Bugle who takes the identity Patch. He likes to do undercover work with Ben Urich in the criminal-underworld
 * Whitney Chang (Sumalee Montano): An investigative reporter
 * Benjamin Urich (Brian Stepanek): An investigative journalist at the Daily Bugle who is somewhat of a crackpot conspiracy theorist to his co-workers and JJ. He has a conspiracy wall at his apartment
 * Amber Douglas (Jessica DiCicco): A young freelance photographer and classmate of Peter Parker at Midtown High. Amber later becomes Eddie Brock's replacement when she took the internship. Amber has long strawberry hair and olive eyes
 * John Jameson/Man-Wolf (TBA): An astronaut for NASA and the son of Jonah Jameson.
 * NYPD
 * Jean DeWolff :
 * Stanley Carter :
 * Yuri Watanabe (Tara Platt):
 * Curt Connors/The Lizard (Dee Bradley Baker): A brilliant scientist and biologist at Empire State University. Conners lost his right arm during the war as an army surgeon for the U.S. Military and has been using a prophetic mechanical arm. Dr. Connors devoted his life to creating a serum that can regenerate lost limbs, using his research on cell regeneration and study of herpetology and reptiles, he created an experimental mutagen using Lizard DNA. After himself as a test subject for the formula, successfully grown a new right arm. But the experiment further mutated Connors, transforming him into an anthropomorphic monstrous reptile known as, The Lizard . As the Lizard, he possesses primitive intellect with superhuman strength, agility, speed, stamina, regenerative healing factor, and highly resistant durability to conventional injury from his thick scaly hide. He can scale on to walls and ceilings with adhesive elements of a gecko. Lizard's mouth is filled with rows of sharp teeth and claws and a long 6.5 foot prehensile tail capable at whipping speeds in excesses 70 mph. His head is that of a raptor/dinosaur..
 * Martha Conners (Cissy Jones): Curt's wife who likes to occasionally helps her husband with his work around the lab.
 * Nathan Conners/Lizard-Teen: An original character introduced to the series. Nathan is the eldest son of Curt and Martha Conners. Nate often helps father around the lab while studying at ESU and he is very protective of his little brother Billy.
 * Billy Connors (Tara Strong): Curtis and Martha's youngest son.
 * Debra Whitman  (Zehra Fazal): A college student who works at the ESU Labs for Dr. Conners as his lab assistant.
 * Farley Stillwell (Jeff Bennett): A biologist and an a colleague of Curt Connors. He was involved in the experiment that transformed Mac Gargan into the Scorpion.
 * Emily Osborn: The wife of Norman who is a environmental attorney and loving mother to her son Harry.
 * Bernard Houseman (Corey Burton): Norman's personal butler in service to the Osborn family.
 * Ashley Kafka (Catherine Taber): A young psychologist that works at the Ravencroft Institute and the love interest of Venom. As Eddie's psychiatrist, Dr. Kafka started therapy sessions for him when he was taken into Ravencroft and determined to help him go through his trouble. Originally, she didn't believe him and tried to convince him that Venom was just a psychological delusion he brought up from the stress and frustration of losing his job and Spider-Man's assumed persecution of him. Ashley tells him that hatred and revenge are not the answer and can't always blame Spider-Man for his own actions of late. When she found out the symbiote was real, she acted to try to stop Eddie Brock from becoming a monster and instead uses his powers to help others. Ashley is a beautiful young woman with brown eyes and short black hair. Her physical appearance resembles her Spectacular Spider-Man iteration.
 * Silver Sable (Jennifer Hale): An international mercenary from the European country of Symkaria, Silver Sable is the field leader of the Wild Pack and the owner of Sable International. Sable has been trained to the peak of near-superhuman perfection in areas of strength, speed, agility, reflexes, and stamina. She is a highly skilled hand-to-hand combatant and martial artist, a lethal marksman, swordsman, and a great athlete and acrobat. She is a natural-born leader and an accomplished intelligent strategist and tactician, capable on analyzing the strengths and weaknesses of her targets.. Silver Sable is a tall attractive and curvaceous woman with flowing platinum silver hair that reaches her mid-back and blue eyes. Sable dons a white sleeveless jacket while wearing a very form-fitting silvery white and gray bodysuit which leaves her shoulders, and light grey arm sleeves that go past the elbows to the biceps. The suit, coat and arm sleeves are made of synthetic stretch fabric lined with protective Kevlar, that provides protection from gunfire, energy and physical attacks. Her suit is based on the Ultimate version, having both her shoulders and upper torso bare to highlight her cleavage.
 * Wild Pack: A Sable international strike team led by Silver Sable and occasionally accompany her. Battlestar, Powell, Chen, Crippler and Paladin serve as commanders. Agents wear black jumpsuits underneath sleek white/silver hi-tech body armor. They have advanced equipment and energy based weaponry. Their transportations are vehicles are helicopters, armed APCs and jetpacks
 * Battlestar/Lemar Hoskins (Ike Ahmadi): Battlestar is second in command of Wild Pack.
 * Douglas Powell (Matthew Mercer): Douglas was a professionally trained western mercenary for-hire with a thick southern accent and pleasure in wearing a cowboy hat in and out of uniform.
 * Amelia "Amy" Chen (Kelly Hu): A former assassin from Vietnam who is Wild Pack's combat specialist.
 * Carl Striklan/Crippler (Xander Mobus): Wild Pack's brute force who is a former wanted fugitive.
 * Paladin/Paul Dennis (Adam Baldwin):
 * Sable Agents (various voices):
 * Madame Web (Susanne Blakeslee): A powerful clairvoyant and psychic mutant who watches over Spider-Man, Kid Arachnid, Ghost-Spider, Venom and Silk for the destiny that lies ahead of them.
 * Ezekiel: A mysterious assailant with spider powers who is an envoy to his ally Madame Web.

S.H.I.E.L.D.
The Strategic Homeland Intervention, Enforcement and Logistics Division is a secret US military law-enforcement agency led by Nick Fury and operates from the Helicarrier.
 * Colonel Nick Fury (Marc Graue): Director of S.H.I.E.L.D. and an acquaintance of Richard and Mary Parker. An American war veteran of the Cold War. Nick was hoping to use Richard Parker's research in hopes of resurrecting the Super Soldier Serum but Richard refused to let Fury uses his discoveries for warfare.
 * Maria Hill (Margaret Easley): Nick's second-in-command and technical officer of S.H.I.E.L.D. as well as old friend of Mary Parker. Maria is Chinese-American descent. She wears a S.H.I.E.L.D. jumpsuit and a bandolier, and has short black hair in a buzz cut style.
 * Agent Phil Coulson (Clark Gregg): An agent of S.H.I.E.L.D. and a mentor to Spider-Man.

The Avengers
A world-renowned team of Earth's Mightiest Heroes that was founded by Iron Man, Captain America, Thor, the Hulk, Black Widow, Hawkeye, Captain Marvel, Ant-Man and Wasp.
 * Iron Man/Tony Stark (Mick Wingert): Founder/Co-Leader of the Avengers, benefactor and billionaire playboy & CEO of Stark Industries who utilizing advanced suits of armor with build-in repulsor weaponry. His personal armor is based on the MCU incarnation.
 * Captain America/Steve Rogers (Roger C. Smith): Founder/Co-Leader of the Avengers and a patriotic super soldier from WWII who was chemically enhanced to the peak of human physicality by an experimental, formula the Super Soldier Serum and was frozen in suspended animation after his battle with the Red Skull and reawakened many decades later in the modern world.
 * Captain Marvel/Carol Danvers (Kim Raver): Founder/Co-Leader and an Air Force pilot whos DNA was altered by an alien race known as the Kree, that turned her into a Superhuman/Kree hybrid abilities and energy and photon based powers.
 * Thor (Travis Willingham): Founder and the Asgardian God of Thunder hailing from the realm of Asgard and the son of the All-Father Odin. Wielding his mystical enchanted hammer Mjolnir, Thor can fly, manipulate and summon basic elements of storms at his will.
 * Hulk (Fred Tatasciore): Founder and a an powerhouse brute that was created by the explosion of Gamma Radiation.
 * Bruce Banner (Kevin Shinick: A brilliant scientist in the field gamma rays that was caught in an explosion of gamma radiation and transforms him into the Incredible Hulk whenever he's feeling angry, stressed.
 * Black Widow/Natasha Romanoff (Laura Bailey): Founder and highly trained spy and special agent of S.H.I.E.L.D..
 * Hawkeye/Clint Barton (Troy Baker): Founder and
 * Scott Lang/Ant-Man (Grant George) Founder
 * Hope Pym/Wasp (Kari Wahlgren): Founder
 * Falcon/Sam Wilson (Zeno Robinson):
 * Redwing: Falcon's faithful pet and companion whose been overseeing Spider-Man's activities.
 * Scarlet Witch (Kate Higgins):
 * Black Panther/T'Challa (James C. Mathis III): The king of the African nation of Wakanda and the current Black Panther.
 * Vision (David Kaye)
 * Tigra/Greer Nelson (Ashley Eckstein): A teenage superheroine and junior Avenger
 * Kamala Khan/Ms. Marvel (Kathreen Khavari) A teenage Muslim-American and Inhuman
 * War Machine/James Rhodes (Kevin M. Richardson): Rhodey is the best friend of Tony Stark and a military officer of US Armed Forces. He is Iron Man's partner as War Machine with his own combat armor created by Tony armed with heavy advanced built in-weaponry and overwhelming arsenal.

Fantastic Four
A family/Superhero team of adventures who gained four individual superpowers after being exposed to Cosmic Ray from a cosmic storm on a space mission.
 * Mister Fantastic/Reed Richards (Wally Wingert): Leader and father-figure of the team and a scientific genius who can bend, stretch, twist and reshape his body in incredible lengths.
 * Invisible Woman/Susan Storm (Caitlin Glass):
 * Human Torch/Johnny Storm (Christopher Jacot):
 * The Thing/Ben Grimm (Dave Boat):

X-Men

 * Wolverine/Logan (Steven Blum)
 * Professor X (Jim Ward):
 * Storm (Mara Junot):
 * Cyclops/Scott Summers (Nolan North):
 * Beast/Hank McCoy (Fred Tatasciore)
 * Jean Grey (Jennifer Hale):
 * Rogue (Kieren v.d. Blink):
 * Gambit (Rick Pasqualone):
 * Jubilee (Danica McKellar)
 * Nightcrawler (Liam O'Brien)
 * Shadowcat/Kitty Pryde (Danielle Judovits)
 * Lockheed (Dee Bradley Baker)
 * Iceman/Bobby Drake (Jeremy Shada)
 * Colossus (Stefan Kapičić)
 * Psylocke (Erica Lindbeck)

Individuals

 * Pepper Potts (Felicia Day): An employee at Stark Industries and Tony's personal secretary. Pepper is close friend with Rhodey and Tony, whom has semi-romantic relationship with.
 * Luke Cage (James C. Mathis III)
 * Moon Knight (Gideon Emery)
 * Daredevil (Charlie Cox)
 * Elektra (Élodie Yung)
 * Dr. Strange (Liam O'Brien)
 * DJ Khaled

Other Characters

 * Bonesaw McGee: An American pro wrestler who Peter as the Spider defeated in steel cage match.
 * Crusher Hogan: Bonesaw's partner who challenged The Spider when he beat Bonesaw but to no avail.
 * Julia Jameson: The wife of Jonah Jameson and mother of John Jameson.

Central Antagonists

 * Green Goblin/Norman Osborn (Troy Baker/Mark Deakins): One of the series' main antagonists and archenemy of Spider-Man. Norman is the billionaire founder and CEO of Oscorp Industries, businessman, and the father of Harry Osborn. Norman is a ruthless owner of one of the leading chemical and military manufacturing firm based in New York City, which also had research divisions that dealt in other areas. It is unknown whether he had founded the firm or inherited it. One night, Norman Osborn's team of scientists created the OZ formula, an experimental performance enhancer that Oscorp has recently developed and used himself as a test subject. Unfortunately, the formula proved to be unstable and caused Norman to have severe damage to his psyche and created a split personality. The gas then transformed Osborn into an insanely tall slender goblin-like creature as the Green Goblin, one of greatest archenemies of Spider-Man along with Doc Ock and Venom. Norman and Green Goblin have a Jekyll & Hyde relationship that Goblin often speaks to Norman though a mirror or appears to him as a figment of his imagination. The OZ gas has enhanced Goblin's body to superhuman levels beyond natural limits of a human being with strength, agility, relaxes, stamina and endurance. Green Goblin has a high-tech bat shaped Goblin Glider from Oscorp as his personal mode of transportation that has great maneuverability and speed. The glider has a variety of weapons with missile-launchers and a rotating machine gun, and a bag o' tricks, miniature jack-o-lantern themed Pumpkin Bombs and Razor Bats.
 * Dr. Octopus/Otto Octavius (Simon Templeman): One of the series' main antagonists and archenemy of Spider-Man. Otto Octavious was once a renowned and respected scientist, inventor, and nuclear physicist at Oscorp Industries who invented a harness equipped with a set of four 6'5"ft. advanced cyber-robotic tentacles controlled via neural link transmitter chip. The purpose of the arms is to assist Otto for various facilitated multitasking, handling hazardous chemicals and radioactive materials and conduct dangerous experiments from a safe distance. They are capable of great strength, highly precise quick movement, extend up to 24 ft in length and each have three digit finger-like pincers that can grab object of various sizes. The arms are made of pure stainless adamantium, making them highly resistant to heat, damage, radiation and magnetism. Otto can also use the arms for traversing horizontal distances, able to scale up walls and high places. Tow years ago, Otto was conducting a experimental presentation at Oscorp Labs with a prototype fusion-based energy reactor for Oscorp until an horrible accident occurred, the reactor became unstable and Norman had to seal the lab with Otto sill inside. The leaked radiation then caused a horrific explosion that seemingly killed Dr. Octavius. Thought to be deceased, Octavius had survived the ordeal but the radiation had permanently fused the harness to his spinal cord and caused severe irreversible brain damage that turned the respected scientist into an insane criminal and plans to exact revenge on Norman Osborn for abandoning him and became the diabolical and intellectual criminal mastermind, Doctor Octopus or "Doc Ock" for short. He then turn his attention to Spider-Man later became his formidable adversary. Prior to his criminal career, Otto was good friends with Richard Parker before he departed from Oscorp. Otto has short dark brown hair and olive eyes and wears a jumpsuit with a dark grey and orange color scheme underneath a brown trench coat with the arms sticking out from the back and a pair of red lensed lab goggles. He's the founder and leader of the 1st Sinister 6 and later the Sinister 9.
 * Wilson Fisk/The Kingpin (Bob Joles): One of the series' main antagonists. Wilson Fisk is a calculating and feared heavyset crime lord as New York's Kingpin of Crime. In the public image, Wilson is a wealthy and benevolent entrepreneur, respectable philanthropist and CEO of Fisk Industries. He has been running New York for many several years only to noticing Spider-Man has been meddling in his empire. So he enlist the likes of Norman Osborn to create supervillains as a means to keep Spider-Man's attention occupied. Fisk is a very tall and imposing heavyset man whose body mass is only "2% fat", a statement followed by the declared possession of "350 pounds of muscle." His clothes is normal business attire, preferably in a white suit with a lapel purple tie. He has a walking cane with a built-in laser.
 * Hammerhead (John DiMaggio): Kingpin's right hand man and chief flat-topped enforcer whose skull implant is made of pure adamantium. His clothes are somewhat like a gangster from the 1920s. He wields a Tommy gun-themed plasma rifle, steel knuckles.
 * Alistair Smythe (Nolan North): A prodigy
 * Vulture/Adrien Toomes (Kristoffer Tabori): A senior scientist/inventor and colleague of Otto Octavius, who worked in the Aerospace Division of Oscorp. Adrien built himself a black/red vulture-themed flight suit with a special harness set with robotic wings with razor-sharp blade-like fathers that can attach/detach through energized magnetic means thus used as projectiles from the wing tips, boot-mounted talons and it's unknown power source gives him enhanced strength and longevity. The wings allow Toomes to fly high speeds up to 95 mph. Toomes maybe a-bit quite old in his age, but he's spry. His harness was upgraded with a impact jet engine. His suit is based his MCU version only with some influences on his Spectacular and Insomniac counterparts.
 * Sandman/Flint Marko (Rick D. Wasserman): A common thug who was constantly caught by Spider-Man at ease. However, as he was escaping from prison, Flint found his way in a testing facility experimenting on bonding separate materials. Flint, unknowingly, stowed away inside a tank of irradiated sand, bonding itself to his molecular structure. Once awakened from his daze, Flint noticed his new found abilities in morphing himself in many ways imaginable. He now gets away with more crime by sneaking into heavily-guarded areas and steal anything he can get his sandy hands on as the Sandman.
 * Electro/Max Dillon (Mark Hildreth): Max Dillon was a young electrician and an Oscorp employee with Electrical superpowers. One day on the job, Max was repairing a computer terminal inside a laboratory during a thunderstorm when a lighting bolt caused the panel to short circuit that shocked Max with strong electricity to make him missed his footing and fell into a large tank filled with genetically altered bio-electric eels and then was being mauled and electrocuted by them simultaneously while franticly trying to get out of the water. Although he survived the accident, Max's body was imbued with streams of powerful bio-electrokinetic powers from the eels and became the high-voltage villain, Electro. As a living capacitor, Electro can manipulate and generate massive quantities of yellow electricity per minute up and discharge electrical blasts and lightning arcs from his hands and fingertips. He can absorb the energy from any external power source to recharge his body's limitless energy reserves. Electro has enhanced strength, stamina, durability, endurance and speed when he's running on power lines, He can also electrically charge his physical attacks. Electro can travel at high speeds by generating a massive electromagnetic field which can enable him to levitate through the air and fly. Electro is the most carefree, comedic and laid-back of Spider-Man's foes, as well as arrogant, impulsive, hyperactive, overconfident, charismatic egotist. He shows a sadistic and destructive side as he tends to harm/and torture other for his own amusement and entertainment. Max Dillon is a tall young adult in his mid 20s with lime green eyes and black hair. After his transformation to Electro, his eyes turned golden and his hair have distinctive star pattern streaks that look like lightning bolts, a nod to his traditional mask from the comics. He dons a black leather jacket w/h torn sleeves while wearing a sleeveless black and dark blue Insulated bodysuit with yellow lightning accents on as a nod to the classic and wrist gauntlets. This version is an amalgam of his Amazing Spider-Man 2, Insomniac and classic counterparts.
 * Rhino/Alex O'Hirn (Fred Tatasciore): Alex was a Russian-American mob enforcer who always gets apprehended by Spider-Man during heists, and after hearing about Oscorp wanting to find applicants willing to test experimental battlesuit and work under their rule to defeat Spider-Man, Alex knew if he took this chance he could be the most feared and respected thug in NYC. However, unbeknownst to him, the experimental high-tech rhinoceros-themed combat suit he was testing out involved nanotechnology that permanently grafted to his skin. The armor is now apart of him, having a thick metallic impenetrable hide, immense super-strength and a protective helmet with two razor-sharp metallic horns, Alex becomes he armored brute, Rhino. The suit is highly resistant and impervious to temperatures, explosives and acid solutions, making Rhino an unstoppable force to be reckoned with. Despite his large size, Rhino has heightened superhuman speed, allowing him to run or exercise at high velocities and destroy any obstacle in his path while charging like a battering ram. Being the all-brawn and no-brains type, Rhino is an impulsive, violent, destructive, gruff and short-tempered criminal that uses his massive size to intimidate others. He also has a violent sense of humor. His suit looks the same to his Insomniac counterpart and his size is 7.3 feet.
 * Scorpion/Mac Gargan (Jason Spisak): Mac Gargan was a small-time petty criminal initially hired by J. Jonah Jameson to find out how Peter Parker is able to obtain incredible pictures of Spider-Man, but to no avail. Jameson then decide to use Gargan as a test subject for an "illegal" bankrolled experiment by Dr. Farley Stillwell that would turn Gargan into a superhuman using the DNA of the Scorpion, which was the natural predator of the spider. Stillwell then placed Gargan in a high-tech scorpion-themed battle suit equipped with a cybernetically-controlled, seven-foot prehensile tail armed with a high-powered plasma laser/acid sprayer and a retractable bladed stinger. Mac was enhanced with great strength, speed, agility to super human levels that would prove him to be a match for the web-slinger. However the experiment had fused the suit to Gargan's body, making unremovable and has made him unstable, more aggressive . The suit is primarily dark green with a black underskin, with lime optic lenses has three fingers on each hand and two toes on each foot. Scorpion is a sadistic, suave, merciful and sociopathic criminal . Gargan harbors a grudge against Jameson for the experiment and turned him into a freak with his unremovable scorpion suit for life. His scorpion sui is identical to his Insomniac incarnation
 * Mysterio/Quinten Beck (Daran Norris): A former magician and special effects designer and the most skilled of all. His dreams of becoming a great actor were poorly received, but he realized that his expertise in illusions and special effects could make him an effective supervillain. Quentin had created a suit that helped him create illusions such as duplicating himself and disappearing, powered by the Myst, a special mist created with stolen hard light hologram technology from Oscorp. Wearing a clouded sphere Helmut, complete with a purple cape, Quinten became the self-proclaimed Master of Illusions, Mysterio.
 * Kraven the Hunter/Sergei Kravinoff (Eric Visbit): Sergei is well-known big game hunter from Russian nobility who enjoyed the pursuits life of a socialite and lead the thrill of the hunt growing up in Africa. Over the years he is aided by his children; Alyosha and Ana Kravinoff; their family pet lion, Guyatkin and the shaman, Calypso. While on a safari hunt in the jungle, Kraven was given an elixir from a local shaman, which increased his strength, reflexes and stamina to peak human perfection. . Kraven later traveled to NYC with his family to hunt his ultimate prey, Spider-Man to prove that he's the greatest hunter in the world. After countless defeats at the hands of Spider-Man, Kraven needed more power to be more of a match for Spider-Man's prowess. He was approached by Vermin who offered Sergei a mutagen that transforms him into a black-maned were-lion with the elements of cheetah and leopard DNA. After his mutation, Sergei renames himself as "Kraven the Hunter". Kraven had olive brown eyes and black hair, mustache and goatee and wears a lion-themed vest. He has an array of weapons and advance equipment he uses on his hunts includes his signature spear, a plasma-shotgun, two laser-pistols, a machete, a longbow and arrows, a boomerang, bolas, smoke-grenades, laser netting and a hunting dagger. In his mutant form, he gains the appearance of a humanoid lion with a black mane and tail tip, light golden fur with dark brown stripes on his cheeks and shoulders while muzzle, torso and paws are paler golden with black hair tuft on his chin. He has a black nose and his eyes remain olive with slit black pupils. He has large hands with black retractable sharp claws.
 * Alyosha "Grim" Kravinoff (Matthew Mercer): Alyosha is the son of Sergei Kravinoff. This version is amalgam of Vladimir and Alyosha Kravinoff with Alyosha having the visual elements of the Grim Hunter
 * Anastasia "Ana" Kravinoff (Morgan Laure): Ana is the daughter of Sergei Kravinoff and younger sister of Alyosha
 * Calypso (Pearl Thusi): An African shaman and the lover/girlfriend of Alyosha Kravinoff. She met Grim and his family when when a local warlord tried to kill her and her people.
 * Vermin/Edward Whelan (Phil LaMarr): Dr. Edward Whlan was brilliant geneticist and scientist at Oscorp when injected himself with a mutagen contained with Rat DNA for testing. A certifiable mad scientist who can control rodents. Vermin is the first super villain Spider-Man ever faced. This version of Vermin is more rodent and rat-like than in the comics; he has a rat tail.
 * Hobgoblin/Jason Macendale (Robin Atkin Downes): A former shield agent whose methods and tactics proved too brutal and extreme for Nick Fury his superiors to tolerate. Now without a job, Jason sought for employment and a way to gain money in other ways of business, he became a mercenary-for-hire for those who would pay for his talents. Jason gained great interest of the Green Goblin's recent activities from his battles with Spider-Man. He soon managed to find one of the Goblin's many lairs, this including most of his arsenal, OZ formula notes. By reading Osborn's notes and comparing it with how the Goblin acted in action, Macendale saw a glaring fail in the OZ Formula, the side-effect that was insanity. After few hours of modifying and recalibrating the formula, Jason had removed the insanity-inducing side effects. However the new formula has given him a goblin-like appearance with enhanced strength and abilities and then gained a Oscorp glider and the rest of Green Goblin's gear and weapons cache, Macendale took on the moniker, the Hobgoblin.
 * Shocker/Herman Shultz (Ryan Alosio): A former gang boss under the employ of the Kingpin. He soon built himself a custom suit and stolen Vibro-Shock gauntlets from Stark Industries that releases energized punches, shock waves and compressed air.
 * Clash/Clayton Cole (Roger Craig Smith): Clayton is a former student of Horizon Academy and Herman's fellow protégé with specialization in sonic technology.
 * Stunner/Angelina Brancale (Heather Doerksen): A Canadian college student who became an amazon powerhouse, Stunner. After being fed up with being teased and bullied by her peers, she dropped out of ESU and faded to obscurity. After she went to her apartment, Angelina received an anonymous phone call from an unknown assailant (Mr. Fiers) and bankrolled for an experimental procedure. After arriving to the address; an hidden laboratory.to be She was then used as a test subject for an improved super soldier serum Mr. Fiers somehow obtained. She was then placed in a capsule chamber and injected with the enhanced serum and dosed with large amounts of Vita Radiation. Angelina became the woman she's always meant to be, she emerged as a 6'8" ft. slender well-muscled feminine amazon with a slim waist, buxom breasts, ripped broad shoulders, toned midriff, tight toned abs, strong muscular toned arms, biceps and long legs, Angelina became the villainous Stunner. She has dark brown eyes and long straight blonde hair which reaches down to her waist. The serum has given Stunner enhanced superhuman strength, agility, athletic reflexes, speed, endurance, invulnerability and able to leap long distances. She is strong enough to lift 30 tons. Despite her great durability, she is vulnerable to high concentrated energy attacks. Angelina was once a shy and timid individual whom lacked confidence was treated like an outcast. When Angelina transformed into Stunner, she has gained newfound self-confidence and carefree. But she later became sadistic, arrogant, vindictive, egotistical, sadistic, tease-happy, merciless and ruthless. Angelina's costume is different but similar to her comic incarnation, she wears a red/grey skin tight suit, leaving her shoulders bare and highlighting her décolletage and cleavage. She wears black/red gauntlets, leaving her hands bare and black knee-high boots.
 * Molten Man/Mark Raxton :
 * Carnage/Cletus Kasady (Scott Cleverdon): A mentally insane and deranged psychopath who was apprehended by George Stacy and was imprisoned for twelve consecutive life sentences of rehabilitation at Ravencroft. When Eddie Brock became his cellmate, the symbiote returned to bond with him and Venom make a quick escape, unknowingly leaving an offspring behind. After witnessing this, Cletus was thrilled with joy and wanted the power Eddie possesses, he noticed the small piece of the symbiote and as he picked it up, it bonded though the cut on his arm and transformed Kasady into the Ultimate Insanity, Carnage. Carnage's bond with Cletus is stronger than the Venom symbiote and Eddie and is more violent, powerful and deadly. Besides haing the same powers as Venom and sharp claws, Carnage can create use his symbiote in the form of solid weapons such as darts, spears, axes, knives, and tendrils.
 * Silvermane/Silvio Manfredi (Richard McGonagle): An notorious yet-elderly crime-boss of the Maggia. Silvermane wears a hi-tech battle suit.
 * Mister Negative/Martin Li (Matthew Yang King): The benevolent philanthropist of the homeless shelter F.E.A.S.T. In secret to the public, Martin is a ruthless crime boss and leader of the Inner Demons. When in powered with darkforce, Martin can alter his entire body with the appearance of a photographic negative based on his namesake.
 * Inner Demons: A gang of Chinese immigrants that quickly became corrupted henchmen for Mr. Negative based mainly in Chinatown. They wear elaborated Chinese demon masks and normal black/white business attire. With Mr. Negative corrupted touch, they have been imbued with negative energy with minimum strength
 * Hydro-Man/Morris Bench (Mark C. Hanson): Morris Bench was accidentally knocked overboard by one of his fellow crewmen of the Navy while a powerful experimental generator was being lowered to the ocean floor for testing. Exposed to the energy conversion process of the generator while being bombarded with volcanic gases, Bench's cellular structure was altered. When he was rescued from the depths, his body began exuding great quantities of a watery substance, and soon after discovering his powers, Hydro-Man set out to find those he held responsible for his condition.
 * Coldheart/Kateri Deseronto : A highly skilled mercenary-for-hire and experienced swordswoman wielding Cryonic Blades, two swords that are comprised of cryogenic technology. Coldheart has blue eyes and long black hair.
 * Matthew Golden/Goldbug: Matthew Golden is one of the wealthiest families and businessmen in NYC and the esteemed head of the Gilden Financial Group. Unaware to the public, he is secretly a rival crime lord to Kingpin, Silvermane, and Mr. Negative named Goldbug. Goldbug is extremely obsessed with three things in his life; gold, power and his precious daughter Angela who is unaware of her father's criminal life.
 * Raptor/Damon Ryder : A wanted criminal mercenary and the ex-boyfriend of Silver Sable.

Recurring villains

 * Spencer Smythe (Stephan Stanton):
 * Spider-Slayers
 * Agent Orange/Roger Goshaw : A mercenary who wears a large prototype hi-tech armor stolen from Stark Industries. Three years ago, Tony Stark designed the Multi-Man Armor for construction purposes, allowing one worker to work out of twenty men. Congress canceled it's plan due to costing 20 billon dollars per suit. Somehow the Gentleman obtained the prototype from one of storage facilities own by Stark Industries and brought to Fisk Industries where Alistair Smythe modified the Armor for combat by increasing it's durability and adding various weapons. The Gentleman, Mr. Fiers later recruited mercenary named Roger Goshaw and outfitted him with the advanced Multi-Man Armor to attack Oscorp research facilities as a means to gain Spider-Man's attention. Tom then adopted the alias Agent Orange based on the Armor's color.
 * Nattie/Mendel Stromm (Danny Jacobs): A former Oscorp scientist and colleague of Edward Whelan who spliced his own DNA with genes of a red-bellied piranha. Nattie resembles a humanoid fishman with red eyes, darkish blue skin w/h silver-flecked scales and red underbelly
 * Grizzly/Maxwell Markham (John DiMaggio): A famous and former pro wrestler until he exposed as a cheater in an article from the Daily Bugle. Gustav Fiers bankrolled Max into an experimental trial that mutated him into a large anthropomorphic grizzly bear. After his mutagenic transformation, Markham renames himself to Grizzly. His mutation is similar to the Theodor Winchester incarnation for he is mutated into humanoid brown bear.
 * Pretty Persuasions/Heidi Franklin (Holly Franklin): Heidi was a strip dancer that was taken by GeneTech to be experimented upon and was given enhanced strength, agility and durability. Pretty Persuasions can create tangible psionic energy weapons, usually bondage themed weapons like whips. She can psionically enhance her natural beauty and allure to seduce men. However Spider-Man is immune to her seductive charms. Heidi later escapes Genetech and pursues in her new life as a sexy independent supervillain. Despite her beauty, Pretty Persuasions is a violent and semi-sociopathic woman who enjoys using her powers cause pain to others and getting everything she desires. Pretty Persuasions later forms an likely partnership with Stunner. Heidi has pale skinned, long black hair and dark blue eyes with lime green eyeshadow and wears pinkish red lipstick. She wears a black chocker, black leather strapless-style top, v high cut bottom briefs, thigh-high black boots, leather straps on each arm, all highlighted in purple.
 * Scorcher/Steven Hudak (Michael Jai White): A research scientist and chemist from Alchemax who created an insulated battlesuit arm with flame throwing equipment.
 * Commanda/Catherine D'Antan (Fryda Wolff): An international jewel thief from France with a heart-filled infatuation for Spider-Man. Bored with her idle luxurious life, Lady Catherine became the costumed super thief, Commanda. After successfully pillaging France as a super criminal, She then travels to America in NYC to face new challenges. following her defeat, Catherine escaped the authorities and became immediately attracted to Spider-Man and is now determined to have him for herself. Commanda is a smart, wealthy and a very attractive woman who is an expert seductress that solely relies uses her looks and feminine whiles to take advantage throw her oppressors off-balance. Catherine has a voluptuous yet athletic build, long blonde hair which reaches down to her mid-back, ice blue eyes, toned broad shoulders, buxom breasts, long legs, and wears pale red lipstick. She wears a violet tiara with a centered purple gem that controls an array of robotic drones at her mental commands. Commanda also wears a unique royal orange adjustable armored bodysuit with a purple cape w/h an orange interior. She wears purple hi-tech bracelets that shoots energy beams to stun Spider-Man, rendering him unconscious. Commanda can reshape and alter the appearance of her suit to more revealing outfits at will with the gemstone, leaving her shoulders bare revealing her breasts and cleavage. The stone augments Commanda to have superhuman strength, reflexes and invulnerability.
 * Iguana : An albino Iguana housed in Curt Conner's lab when it crawled out and drank a small portion of Conner's Lizard formula.
 * Crossbones/Brock Rumlow (J.B. Blanc): A mercenary working for HYDRA.
 * Dr. Doom/Victor von Doom (Maurice LaMarche):
 * Batroc the Leaper (Gavin Creel): An common enemy of Captain America who is a master of Savate, the French form of kick-boxing. He was defeated by Spider-Man, Captain America and Kid Arachnid.
 * M'Baku the Man-Ape (Kevin M. Richardson): A fearsome warrior of the Jabari White Gorilla tribe from Wakanda and the hated enemy of the Black Panther. M'Baku gained his great strength from devouring the flesh of a rare and a sacred white gorilla. With Kraven's unseen assistance, M'Baku defeated and killed king T'Chaka, the previous Black Panther and took over Wakanda. His rule was short-lived when T'Challa, the new Black Panther liberated Wakanda with the Avengers. M'Baku then fled from Wakanda out of cowardice and escaped to the Unite States in hiding. He resurfaces and realigns with Kraven and his family again with stealing the Vibranium shipments.

The Sinister Six/Seven
A team composed of six of Spider-Man's formidable villains that was founded and organized by Doc Ock and the King Pin with the sole purpose of destroying Spider-Man. The group was soon followed by it's successor, led by Alistair Smyth. The following successor of the two group became the Sinister 7 led by the Green Goblin

The Sinister XIII
The group successor of the Sinister 6/7. Comprised of 13 villains of Spider-Man, its founded and organized by Doctor Octopus and employed by the Kingpin.

HYDRA

 * The Red Skull/Johann Schmidt (Liam O'Brien): Leader of HYDRA and the archenemy of Captain America. During WWII, He was injected with the imperfect version of the Super Soldier Serum, becoming the first and only Super Soldier of HYDRA. The imperfected serum increased his strength, but it also disfigured his head and face, and he became known as the Red Skull. Upon reading Richard's research on cross species genetics and symbiotic bio suit in creating biological weapons in favor of Hydra: an army of super soldiers enhanced with Animal DNA and.
 * Madame Hydra/Ophelia Sarkissian (Nikka Futterman): Second-in-command of HYDRA and one of Red Skull's lieutenants.
 * Baron Wolfgang Von Strucker (Robin Atkin Downes) Third-in-command of HYDRA and member of the Hydra High council.
 * Arnim Zola (Mark Hamill): HYDRA's Chief scientist.
 * Grim Reaper/Eric Williams (Troy Baker): A dangerous agent of HYDRA. He's outfitted him with a cybernetic arm which can turn into a power scythe.
 * Karl Fiers (N/A): Gustav's deceased brother and a skilled Hydra assassin for Hydra. Karl tried to assassinate Richard & Mary Parker as they were meet Nick Fury at a disclosed location about HYDRA's use of Richard's research. Karl met his fate when the plan crashed in the mountains.
 * Hydra Agents: The foot soldiers for HYDRA

Manhattan

 * Horizon Academy:
 * Faculty: Max Modell, Sajani Jeffrey, Jürgen Muntz, Bella Fishbach
 * Students: Peter Parker, Mary Jane Watson, Gwen Stacy, Harry Osborn, Miles Morales, Flash Thompson, Liz Allen, Randy Robertson, Sally Avril, Glory Grant, Kenny "King" Kong, Jason Ionello, Sean "Tiny" McKeever, Cindy Moon, Angela Golden, Seymour O'Reilly, Jenny Carson, Ralphie Hicks, Hector Cervantez, C.J. Vogel, Polly McKenna, Montana Gold, Louie Minnelli, Stanley Stackmeyer, Amelia Hopkins, Maya Rider, Joey Gastone, Sha Shan, Moose Mansfield, Jennifer Hardesty and Howard Kavanagh.
 * The Daily Bugle:
 * Oscorp Tower: A tall corporate building in Midtown and the headquarters of Oscorp Industries.
 * Empire State University
 * NYPD HQ:
 * F.E.A.S.T.: Food, Emergency, Aid, Shelter and Training is a charity shelter and soup kitchen for both the poor and the homeless operated by Martin Li in Chinatown. It is a place for employment for May Parker and volunteer work for Peter, Miles, Gwen, Harry and Later Cindy Moon.
 * Employees: May Parker
 * Ryker's Isle Prison:
 * The Raft: A highly advanced super-maximum security prison on an artificial island next to Ryker's. It specially designed for imprisoning superpowered criminals.
 * Avengers Tower: The main base for the Avengers and the headquarters or Stark Industries
 * Baxter Building

Queens

 * Aunt May's House:

Other Locations

 * X-Mansion
 * Tangaroa Island
 * HYDRA-HQ

Origin Mini series

 * Spider-Man: A new Beginning, Part 1
 * Spider-Man: A new Beginning, Part 2
 * Spider-Man: A new Beginning, Part 3

Season 1(26): New Beginnings

 * 1) New Horizons, Part 1:
 * 2) New Horizons, Part 2:
 * 3) Of Mice and Vermin
 * 4) Scorpion's Sting
 * 5) Wings of Vengeance
 * 6) Unbreakable Hide
 * 7) The Cat's Meow
 * 8) 8-armed and Dangerous:
 * 9) A Lizard's Tale
 * 10) Illusions of Grandeur
 * 11) Spider Slayers
 * 12) The Black Suit, Part 1:
 * 13) The Black Suit, Part 2:
 * 14) The Black Suit, Part 3:
 * 15) Stunner:
 * 16) The Ultimate Spider
 * 17) Nattie's Frenzy
 * 18) Shocking Vibes:
 * 19) Changing Sands
 * 20) Six Against One, Part 1:
 * 21) Six Against One, Part 1:
 * 22) Oh Captain My Captain:
 * 23) Kingpin's Gambit
 * 24) The Darkness Uniting
 * 25) Rise of the Green Goblin, Part 1
 * 26) Rise of the Green Goblin, Part 2

Season 2(26): New Alliances

 * 1) The Man in the Shadows:
 * 2) Glitters of Gold
 * 3) Scorched
 * 4) Commanda-in-Chief:
 * 5) Ghost-Spider's Beginning
 * 6) Hunted:
 * 7) A Goblin Amongst Us
 * 8) Horns or Stingers
 * 9) Hydro-Tide
 * 10) Open House
 * 11) Shield we Trust
 * 12) Venom Returns (Part 1):
 * 13) Chaos in-Carnage (Part 2):
 * 14) Lethal Protector (Part 3):
 * 15) A-Day
 * 16) Spider-Man's Fantastic Adventure:
 * 17) Goblin Battle Royal
 * 18) Web-Warriors Di-Sabled:
 * 19) Clashing Sonics:
 * 20) The Spiders, The Hunter and the Panther: Spider-Man, Kid Arachnid and Ghost-Spider team up with the Black Panther to track down Kraven the Hunter, Alyosha and Ana who have shipment of stolen Vibranium with their associate, M'Baku the Man-Ape. Unaware to our heroes, Kraven and Man-Ape are about to got through mutagenic changes by their new Allies
 * 21) Gwen Undercover:
 * 22) Agent-O Standing By:
 * 23) The Secrets of Richard and Mary:
 * 24) Smythe's Slayer Six (Part 1)
 * 25) Slayer Six Silked (Part 2)

Season 3(26): Mutants and Gang Wars

 * 1) F.E.A.S.T. or Demons:
 * 2) The Raft:
 * 3) Scarring Partners
 * 4) Cold-Hearted
 * 5) Doc Ock's Voyage
 * 6) Demolition Days
 * 7) Kraven's Gauntlet
 * 8) Sister
 * 1) Sister

Season 4(26)

 * 1) Split Decisions
 * 1) Split Decisions
 * 1) Split Decisions

Trivia

 * In this series, Spider-Man's parents, Richard and Mary survived their plan crash and supposedly faked their deaths. The two have been in hiding for years from Hydra and it will the first time that Peter Parker will be interacting with his mother and father in person.
 * Instead of using virtual reality technology, Stunner gets her powers from an enhanced form of the Super Soldier Serum.
 * The other wrestlers at venue made cameo appearances were Bronx Mangler, Fatal Finnegan, Halcon D'Oro, Cowgirl Lowry, Dawn Vavavoom, Letha and Magilla Sandy.