Girls Rule/Season 1

The first season of Girls Rule premiered worldwide on the streaming service HBO Max on October 28, 2022.

Recurring

 * David Hayter as Boneblaster, a new supervillain from Gotham. He gets to have a few run-ins with the girls and develops a grudge against Catwoman before she defeats him.
 * Conan O'Brien as Riddler, a Gotham City criminal mastermind, he colludes with Hush in his plan until he betrays him. He ends up in Arkham Asylium after a confrontation.
 * Will Friedle as Maxwell Burton, a STAR Labs doctor who discovers Poison Ivy by investigating hidden files. After passing some incidents, both become boyfriends.
 * Héctor Elizondo as James Gordon, the retired police commissioner of Gotham and Barbara's father.
 * Jason Liebrecht as William, a bank manager who was robbed by Harley Quinn. Later he is one of those kidnapped by Elliot.
 * Griffin Burns as Tony, a bodyguard of the Joker.
 * Fred Stoller as Harold Allnut, a genius partner of Batman's for a long time. He is manipulated with pheromones to betray Batman, then redeems himself.
 * Kate Micucci as Clayface, one of Batman's villains, he is mind-controlled by Hush and joins as one of his henchmen, before escaping.
 * Billy Dee Williams as Two-Face, he briefly appears as a controlled ally of Hush.
 * Ralph Garman as Lowery Arnet, a scientist in charge at STAR Labs and the experiments performed there. He is remote and controlling with his employees.
 * Chris Hardwick as James Olsen, a Daily Planet television reporter.

Guest

 * Billy West as Alex, a Daily Planet interviewee
 * Patrick Warburton, as Orlando, a kidnapped.
 * Kath Soucie, as Miranda, a kidnapped.