List of characters in Looney Tunes: Acme Oop!

Bugsworth "Bugs" Bunny

 * Voice Actor: Billy West

The main protagonist and central character of the series. Bugs is a grey rabbit who wears pale yellow gloves which are a stark contrast to his previous appearances in which he wore white. His personality in the series retains his charismatic trickster ways while combining it with a more easygoing nature, with his design being more reminiscent of Bob Clampett's. Bugs is usually forced to spend his days evading Elmer Fudd's constant attempts to cook him into rabbit stew. He resides in his own comfortable rabbit hole on top of a small hill deep in Clampett Forest. Bugs is best friends with Daffy Duck, despite finding his craziness to be a nuisance, and Porky Pig, whom he often takes for granted. His main rival is Elmer Fudd, with his wisecracks annoying the hapless hunter to no end, and he is also currently engaged in a bitter feud with Cecil Tortoise. Bugs' main catchphrase is "What's up, doc?"

Daffy Dumas Duck

 * Voice Actor: Eric Bauza

The deuteragonist of the series. Daffy is a black duck with a curved beak and a grey pearl choker which he has worn since birth. He is once again returned to his screwball roots for the series, causing his design to harken back to the days of Frank Tashlin and Clampett, and his personality to be extremely unpredictable and crazy, but he still has moments of sanity throughout. Daffy is not very popular among Acme Acres' other residents for his general weirdness and oddball tendencies. He makes his home in the very same duck-shaped boat from 1949's Daffy Duck Hunt. Daffy's only friends are Bugs Bunny, who secretly finds him annoying, and his "sidekick" Porky Pig, with whom he seems the most compatible. He also seems to be an outright bother towards Elmer Fudd. Daffy's main catchphrase is his signature laugh of "Woo-hoo, woo-hoo, woo-hoo!"

Porktholomew "Porkus" J. Pig

 * Voice Actor: Bob Bergen

The tritagonist of the series. Porky is a rotund pig clad in a navy-blue jacket, a red bowtie and a pair of amber gloves. Out of all the characters, his personality is the most consistent, staying true to the everyman qualities of previous depictions; this also sticks true with his design being closer to Clampett and Tashlin's. Porky is well-liked among the residents of Acme Acres but has a hard time getting along with them due to his ongoing stuttering problem. His place of residence is mostly the same as his house in New Looney Tunes, but with a few tweaks. Porky's girlfriend is Petunia Pig, who has a more positive personality, and his best friends are Daffy Duck and Bugs Bunny, who consider him to be their sidekick. His main catchphrase is "Th-th-th-th-th-th-that's all, folks!", which also acts as his trademark sign-off.

Yakko Warner

 * Voice Actor: Rob Paulsen

One of the two secondary tritagonists of the series. Yakko is a cartoonus characterus who wears a pair of smoky topaz slacks. He's still as talkative, cocky and prone to adult innuendos as his original and reboot portrayals but received a slight redesign to fit the series that re-coloured his gloves to yellow and his nose to crimson, as well as enlarging his feet. In the series, he and his siblings were forced to remain as inanimate black and white drawings for their absurdist personalities but were resurrected after Bugs and Daffy restored their colouration. He lives with his siblings in the the water tower of Schelesingerwood Studios, sleeping in a ball pit underneath Dot's loft. He and his siblings are very close with each other and view their co-workers as a one-sided surrogate family. Yakko's main catchphrase is "Goodnight, everybody!"

Wakko Warner

 * Voice Actor: Jess Harnell

One of the two secondary tritagonists of the series. Wakko is a cartoonus characterus sporting a red baseball cap and a blue turtleneck sweater that speaks in a Liverpool accent. His appetite is still the same from his original and reboot portrayals; he also received a redesign that turned his gloves cream and enlarged his feet. In the series, he and his siblings were forced to remain as inanimate black and white drawings for their absurdist personalities but were resurrected after Bugs and Daffy restored their colouration. He sleeps in a hammock in the Schlesingerwood Studios water tower. He and his siblings are very close with each other and view their co-workers as a one-sided surrogate family. Wakko's main catchphrase is "Faboo!"

Louisa Francesca Banana "Dot" Fanna Bo Besca Warner III

 * Voice Actor: Tress MacNeille

The tertiary tritagonist of the series. Dot is a cartoonus characterus that wears a pink skirt and a flower hairband wrapped around her ears. She is more of a diva compared to both the original series and its' 2020 reboot and was redesigned to have her nose become a reddish orange with her feet being enlarged as well. In the series, she and her siblings were forced to remain as inanimate black and white drawings for their absurdist personalities but were resurrected after Bugs and Daffy restored their colouration. Her bed in the Schlesingerwood Studios water tower is a loft located just next to Wakko's hammock and above Yakko's ballpit. She and her siblings are very close with each other and view their co-workers as a one-sided surrogate family.

Tweety Pie

 * Voice Actor: Eric Goldberg

The tetartagonist of the series. Tweety is a small yellow canary with small eyelashes that make him easily mistakable for a female. He is made slightly more sadistic than previous portrayals, but still retains his cute facade, combining both Clampett and Friz Freleng's interpretations of the character. Tweety's daily routine is often having to thwart Sylvester's constant attempts to eat him. He lives in a birdcage that hangs just outside the ledge of Granny's house in New Freleng City. Tweety is very close with Granny, who happens to be his owner, and is also frenemies with Sylvester, though they will team up if they have to. His main catchphrase is "I thawght I taw a puddytat."

Sylvester James Pussycat

 * Voice Actor: Bill Farmer

The secondary tetartagonist of the series. Sylvester is a black and white tuxedo cat with yellow eyes and a bulbous red nose. His personality in the series retains the gluttonous persistence and sloppiness of previous portrayals; his design, however, is more akin to 1946's Kitty Kornered, a short directed by Clampett. Sylvester's primary aim in life is to catch and eat Tweety, having a lisp that causes him to slobber every time he speaks. He lives with Granny as her adoring yet deceptive house cat but has been known to scrounge in the trash cans outside her house as well. Sylvester is mostly frenemies with Tweety, though he will join forces with him from time to time. His main catchphrase is "Suffering succotash!"

Road Runner

 * Voice Actor: Carl Greenblatt

The pentagonist of the series. Road Runner is a greater roadrunner with a light blue plumage and an oversized dark blue head crest. He retains most of his original positive attitude, but is now able to speak fluently, replacing most words starting with B with a simple beep, and he is given a slight arrogant streak; his design is now a blend of the classic Chuck Jones design and the later Rudy Larriva design with a hint of Clampett's influence. Road Runner is Acme Acres' fastest resident and is not even shy in expressing it. He is also rather partial to birdseed and resides beside a nearby canyon in a painted tunnel which only he can enter. Road Runner is the lifelong frenemy of Wile E. Coyote, who often attempts to eat him through the usage of ACME products. His main catchphrase is "beep, beep!", which he often says when he has to dash off.

Wile Ethelbert Coyote

 * Voice Actor: John Paul Karliak

The secondary pentagonist of the series. Wile E. is a coyote with permanent red bags around his yellow eyes. His passion for invention and snooty demeanour remains part of his overall character and his design stays true to 1949's Fast and Furry-ous, directed by Jones, while being enhanced with the Clampett touch. Wile E. is the ACME Corporation's most faithful customer, relying on their products for almost everything in his daily life. He lives in a giant rock where he usually spends the day thinking up plans for his latest contraptions. Wile E. has an unhealthy relationship with his frenemy Road Runner, who he is constantly trying to capture for his lunch, and looks down upon Bugs Bunny for his less advanced lifestyle. His main catchphrase is "Wile E. Coyote, super genius!"

Pinky

 * Voice Actor: Jim Conroy

One of the two tertiary pentagonists of the series. Pinky is a white laboratory mouse with light blue eyes and a goofy grin forever glued to his face. He's still as imbecilically open-minded and moronic as his portrayal from the original Animaniacs and its' 2020 reboot, but recieved a slight redesign to fit the tone of the series. Pinky is the humbly idiotic sidekick to the Brain, but his dimwitted nature and constant bumbling often cause his master's plans to go disastrously wrong. He lives with the Brain inside a cage in the research facilities of ACME Laboratories. Both mice have a complex relationship with each other; Pinky's stupidity incites Brain's anger towards him, but the two of them would feel completely lost without each other. His main catchphrase is "Narf!"

The Brain

 * Voice Actor: Maurice LaMarche

One of the two tertiary pentagonists of the series. The Brain is a white laboratory mouse of diminuitive stature with a crooked tail and an expansive brain, hence his name. His evil genius and disregard for others is carried over from previous portrayal, along with a minor redesign. Embittered from having been abused by scientists when he was a puppy, The Brain sought to seek control of himself and the entire world along with him, He lives with his sidekick Pinky inside a cage in the research facilities of ACME Laboratories. The Brain regularly abuses him for his overall stupidity, but knows that, without Pinky, he's only half a mouse.

Elmer J. Fudd, Jr

 * Voice Actor: Jeff Bergman

The anti-hero of the series. Elmer has a very large bald forehead that once had blonde hair and usually wears a brown hunting outfit with an oversized deerstalker hat. He is returned to his more mild-mannered personality in place of his flanderization in Looney Tunes Cartoons, utilising the same design from said series, pink nose included. Elmer's main hobby is hunting Bugs Bunny so he can cook him into rabbit stew, but he also enjoys fishing in Acme Acres' rivers. He claims to live in an enormous mansion, but his home is actually a homely log cabin deep in Clampett Forest. Elmer seems to despise Bugs and Daffy and sees them as a constant pain, but he begrudgingly allies with them when needed. His main catchphrase is his signature laugh of "Huh-huh-huh-huh-huh-huh-huh"

Aloysius Bartholomew "Yosemite" Samuel Michaelangelo Rosenbaum

 * Voice Actor: Fred Tatasciore

The primary antagonist of the series. Yosemite Sam is of a short stature, wearing a traditional cowboy outfit that varies in colour and an enlarged Stetson hat to boot. His greed, cranky nature and animosity are extremely heightened; design-wise, he resembles in earlier appearances in the 1940s'. Yosemite Sam is Acme Acres' resident recluse whose temper prevents him from having any visitors, but he actually seems to like being alone. He lives in a ramshackle little house where he counts his ill-gained money and puts up warning signs to preserve his solitude. Yosemite Sam has no known friends and is enemies with every single character with Bugs being his primary target.

Theodore "Taz" Tasmanian Devil

 * Voice Actor: Jim Cummings

Taz is a brown tasmanian devil with two tufts of hair shaped like horns and a body that also acts as his head. He still has his familiar bad temper, stupidity and untamed savagery that makes him very impatient with his design baring resemblance to his debut in 1954's Devil May Hare. Taz has an infinitely fercious appetite that causes him to hunger for just about anything around him and mostly communicates in a series of rasps, growls and grunts. His habitat is actually a very large cave in the Larriva Jungle which he has renovated to be completey disheveled. Due to his carnivorous tendencies, most of Acme Acres' residents tend to avoid Taz for fear of being eaten alive, with only Bugs and Daffy able to interact with him.

Beaky Killer Buzzard

 * Voice Actor: Mike Ruocco

Beaky is a buzzard with black feathers covering his body and a tuft of white feathers surrounding his neck which bends to create a lump in his throat. He is still as simpleminded and gullible from previous portrayals, with his design taking cues from 1942's Bugs Bunny Gets the Boid, and 1945's The Bashful Buzzard. Beaky is often seen flying in mindless ignorance and looking for his next meal but is actually rather harmless and gets along with the other Tunes pretty easily. He lives with his dear Mama in a cave on top of a mountain located in Jones Valley. Beaky has a strained relationship with Mama, who often abuses him for his simpleton nature.

Cecil Tortoise

 * Voice Actor: Keith Ferguson

Cecil is a green tortoise who wears a shell that acts as his everyday attire. He still keeps his amicable disposition but appears to be more cocky and self-centred when compared to previous depictions, with his design taking cues from Tex Avery and Clampett's interpretations. Cecil has made quite a name for himself in Acme Acres, being famed for his seemingly unmatched speed; this is all an act just so he can feel less self-conscious about his own species. He lives in a humble oak tree with his usually neglected wife in Clampett Forest. Cecil is Bugs Bunny's worst rival and is unafraid to rub his ever-growing victories in the envious rabbit's face.

Petunia Pig

 * Voice Actor: Lara Jill Miller

Petunia is a pig with black hair tied in pigtails who wears a purple dress, and red hairbows. Compared to her sparse appearances in the Golden Age, she is given a more positive, cheery personality; however, her design resembles 1939's Naughty Neighbors, directed by Bob Clampett. Petunia is the most joyful resident in all of Acme Acres and regularly enjoys spending time in her garden and engaging in outdoor activities. She lives in a recoloured version of Porky's house which is right next door. Petunia is Porky's sweet-natured girlfriend, having been at his side since childhood, but seems to find Daffy to be an annoying third wheel in their relationship. Her main catchphrase is "You'll never see a rainbow if you're looking down!"

Foghorn Leghorn

 * Voice Actor: Jeff Glen Bennett

Foghorn is a very large leghorn with white feathers covering his body and crimson feathers that distinguish his head. He still keeps his trademark loudmouthed and bossy personality, with his design taking cues from his early appearances in the late 1940s' while being given the Clampett treatment. As the tallest in MicKimson Farm and leader of the chickens who reside there, Foghorn is very proud of his Southern heritage and can often be found singing twisted versions of "Camptown Races". He lives in his own chicken shack within the limits of the farm's chicken coop; said shack is much larger than the rest. Foghorn's two best friends are Chicken Boo, who usually takes the role of his sidekick in the series, and Barnyard Dawg, despite playing crude jokes on him. His main catchphrase is "Aaah, shaddap!"

Chicken Boo

 * Voice Actor: Danny Jacobs

Chicken Boo is a human-sized rooster with tan feathers that help to distinguish his head and tailfeathers. from the rest of his body. He still keeps his undying curiosity of the outside world but is now able to speak received a slight redesign along with the other Animaniacs. Born with the arrogant delusion that he is a master of disguise; Chicken Boo feels like he doesn't fit in with his fellow chickens and tries to make a new life for himself elsewhere. As he is quite large, he tends to sleep in Foghorn's shack without his permission due to him being unable to fit in any of the other coops. Despite this, Chicken Boo is actually Foghorn's best friend and sidekick, accompanying him on his rounds or tormenting Barnyard Dawg.

George P. "Barnyard" Mandrake

 * Voice Actor: Roger L. Jackson

Barnyard is a basset hound who is sometimes seen wearing a collar. His feisty yet dour attitude are still present, but he is made slightly more grouchy than previous portrayals, with his design staying true to his early appearances in the 1940s'. Barnyard is usually the first to voice his personal doubts at whatever his fellow Tunes are doing but does nothing about it because he knows it will go horribly wrong. He lives on McKimson Farm in a cosy little kennel, to which he is chained when not doing his rounds. Barnyard's main abuser is Foghorn Leghorn, who takes pleasure in whooping the dog's bottom with a wooden plank and allowing him to give chase until he reaches the rope limit.

Emma Webster

 * Voice Actor: Candice Milo

Better known as just Granny, she is in her mid-seventies and wears an indigo schoolmarm gown and spectacles with grey hair styled in a bun. Her good nature allows her to take a motherlier position amongst the other characters, and her design takes inspiration from her appearances in the 1950s'. Granny is regularly seen as an old-fashioned spinster who is past her glory days, but she has been known to get up to some pretty wild antics when no one is looking. Her place of residence is a quiet little house that is located in New Freleng City. Granny is Tweety's adoring owner, protecting him from Sylvester's hungry advances and getting the same respect in return.

Ralph Theodore Guard

 * Voice Actor: Frank Welker

Ralph has a very obese build and, like most law enforcement in animation, wears a cerulean uniform which he seems to be too fat to even wear. His good nature and dim-witted naivete keep in line with the original Animaniacs and its' 2020 reboot, but his design got a complete overhaul to meld with the series' style. Ralph is the oafish guard of the Schlesingerwood Studios movie lot, tasked with keeping the Warners' irascible silliness in check, but his incessant bumbling and friendly stupidity regularly put him at the risk of being fired from his job. His actual home is unspecified; however, he is usually found working at the studio kiosk. Ralph is mostly on friendly terms with the Warners, but their antics irritate him sparingly.

Pete Puma

 * Voice Actor: Stephen Stanton

Pete is an orange puma with a tuft of hair sprouting from his head and a somewhat choked-up laugh. He still keeps his stupidity and merely basic intellect; his design is a Clampett-ized version of his original appearance in 1952's Rabbit's Kin. Despite believing himself to be a very skilled hunter, Pete is not exactly one of Acme Acres' smartest residents, with his clumsiness and constant starvation often annoying those around him; he still tries to help them out on certain occasions. He lives in a cave within the darkest depths of Clampett Forest. Pete does not have that many friends or allies but doesn't actually mean any harm unless he gets hungry.

Commander Marvin X-2

 * Voice Actor: Frank Todaro

Marvin is a black martian that wears a red jumpsuit with green Roman armour and white basketball sneakers. His soft-spoken politeness and knack for destruction are still present and his design is reverted back to his debut appearance from 1948's Haredevil Hare with enlarged eyes and lime green gloves. Hailing from the Selzersphere, Marvin often visits to conduct experiments on unsuspecting test subjects and believes his race to be more superior than others. His base of operations is an enormous spaceship that houses his equipment, as well as many more smaller space shuttles, such as the Martian Maggot. Due to only visiting from time to time, Marvin does not know the other Tunes personally.

Nora Rita Norita

 * Voice Actor: Stephanie Escajeda

Nora has a rather slender body, is of Latina descent, and wears a dark blue suit with purple framed glasses. True to her name, she is still as no-nonsense and disciplined as she was in the 2020 reboot of Animaniacs, but received one of the more drastic overhauls in terms of her design to blend with the series' aesthetic. Nora is the CEO of Schlesingerwood Studios but is more concerned with her own beauty than the state of the studio itself. Her place of residence is unknown, but she is mostly seen in her personal office situated in a skyscraper owned by the studio. Nora acts as a surrogate mother to the Warners, with whom she finds a constant pain to coexist.

Rudolph Gossamer the Tennis Shoe Monster

 * Voice Actor: Jhonen Vasquez

Gossamer is a large monster made entirely out of dark ginger hair with yellow fingernails who wears a pair of dull white sneakers. He is made slightly more docile and friendly than previous appearances with his design combining aspects from both 1946's Hare-Raising Hare and The Looney Tunes Show. Having been abandoned by his original creator, Gossamer spent most of his life in isolation until he was discovered by Bugs, Daffy, Elmer, Wile E., and Road Runner. As of now, he lives alone in a haunted mansion located in the Realm of Levitow. Gossamer has been recently trying to make friends with the other Tunes, but they usually run away in fear due to his scary figure.

Doctor Otto von Scratchansniff

 * Voice Actor: Dee Bradley Baker

Scratchansniff has a rather slim build, wears black-rimmed glasses as well as a labcoat with purple buttons and pants, and has a thick German accent. He's still as stressed and prone to insanity as his portrayals from the original Animaniacs and its' 2020 rebot, but his design was slightly overhauled to make him more suitable for the series' style. Scratchansniff is the head psychiatrist of Schlesingerwood Studios and helps out those who work there with their own personal problems, much to the detriment of his own self esteem. His place of residence is unconfirmed; however, he is normally found in his own personal psychiatrist's office that belongs to the studio. Scratchansniff acts as the surrogate father of the Warners, meaning he has to put up with their buffoonery every single day.

Ace Gremlin

 * Voice Actor: Quinton Flynn

Ace is a yellow gremlin who wears a blue aviator's cap and red gloves. His penchant for destruction and amicably unhinged agenda remains unchanged, with his design staying the same as his only appearance in 1943's Falling Hare; albeit with customisable ears and tail to help him adapt to almost any vehicle. Ace is one of the last living specimens of the Gremlin race, who were wiped out when their tropical home was destroyed by loggers; he has since vowed to destroy all machines to avenge his people. He resides in an abandoned plane but is often found all over Acme Acres. Most of the other Tunes find Ace to be an overall nuisance, especially Bugs Bunny.

Sam R. Sheepdog

 * Voice Actor: Jon Bailey

Sam is a rather burly beige Briard sheepdog with a mop of ginger hair that mostly covers his eyes. His personality has been heavily altered from previous portrayals, resulting in him becoming even more lazier than usual; his design is now a combination of 1953's Don't Give Up the Sheep and 1956's Steal Wool, albeit given the Clampett touch to suit him better to the series' style. Sam works at a day job from 9 to 5 protecting a flock of sheep from predators such as Ralph Wolf, which is something he wishes more than anything to get out of. He makes his home in a cave with his unseen wife and puppies which he dug underneath the very hill where he makes his rounds. Sam regularly abuses Ralph for trying to kidnap his entire flock; they are surprisingly very good friends when not at work. He often uses Bugs Bunny to stand in for him as an excuse to spend time with his family.

Ralph G. Wolf

 * Voice Actor: Cosmo Segurson

Ralph is the almost-identical cousin of Wile E. Coyote, the only differences being that he has lighter brown fur, white eyes and a red nose. He remains mostly the same from the original shorts, but is given a broader work ethic, with his design being completely overhauled in the Clampett style with only a smidgeon of similarity to the Jones aesthetic. Ralph mostly doesn't want anything more than his sustainable day job of trying to kidnap a flock of sheep while Sam Sheepdog isn't looking. He cohabitates with the sheepdog and his family in the homely cave they have come to call home. Ralph is usually the brunt of Sam's pugilistic advances for his attempts at ovine homicide, but they are actually on good terms with each other.

Buddy

 * Voice Actor: Scott Menville

The overarching antagonist of the series. Buddy is based on the 1930s' animation aesthetic wears a red jacket with a black bowtie. Instead of the bland, cheery disposition he had in the original shorts, he is given a more selfish, villainous, and greedy personality with his design resembling both his early years and his appearance in the Animaniacs episode The Warners' 65th Anniversary Special. Having had enough of being pushed aside in favour of the other Tunes, Buddy captured all of them except the main and supporting cast, believing if he couldn't have his chance in the spotlight, then no one else could either. His place of residence is currently undecided as he is not an official Tune yet. Buddy is reviled by the rest of the cast for his evil behaviour, made even worse by the fact that he originally had no personality.

Julia "J-37" Brain

 * Voice Actor: Maria Bamford

Julia is a genetically altered laboratory mouse that has cream fur with brown accents and brunette hair. Her personality has become much calmer this time around, having abandoned most of the insanity she developed from the Brain's abuse, with his design remaining somewhat similar to the 2020 reboot of Animaniacs, but altered to better fit the series. Julia can be seen as a complete composite of Pinky and the Brain, as she easily keeps her cool in a crisis when not under stress. Her secret hideout is located inside a hole at the bottom of the tallest tree in the Clampett Forest. Julia once had a romantic relationship with the Brain; the two have now split but they still remain on friendly terms.

Gabriella

 * Voice Actor: Lauren Lapkus

Gabriella is an orange alien lifeform that resembles an aquatic slug with one eyestalk. Her personality still manages to retain the goofiness and positive energy that made her likeable in her previous appearance, along with her design staying true to the 2020 reboot of Animaniacs, albeit with some changes. Despite her slightly repulsive appearance, Gabriella does not actually desire for world domination at all; she instead aspires to be a world-famous pop musician. She originates from a far-off unknown planet from across the Selzersphere that was recently obliterated. Gabriella gets along rather well with Pinky in spite of his stupidity but does not seem to enjoy The Brain's constant plotting for world-domination. She also holds a grudge against Starbox for allowing his race to devour her home world.

Meredith

 * Voice Actor: Katie Rice

Meredith is a pink alien lifeform of a spherical shape that has three eyes and two purple horns. In stark contrast to her debut where she was portrayed as a mere pet, she's much more deadpan and sarcastic and is now able to talk, but her design is mostly the same as the 2020 reboot of Animaniacs, but improved to better fit the series. Meredith is the questionably loyal pet to Gabriella, always accompanying her whether she likes it or not. Like her mistress, she originates from a far-off, unknown planet from across the Selzersphere that was recently obliterated. Meredith doesn't exactly share the superstar aspirations of her mistress, but also holds the very same grudge against Starbox for consuming her former home.

Mama Buzzard

 * Voice Actor: Rachel Butera

Mama is a taller buzzard with black feathers covering her body and a tuft of white feathers surrounding her neck which bends to create a lump in her throat. She still maintains her overbearingly pushy disposition, with her design taking cues from 1924's Bugs Bunny Gets the Boid, and 1945's The Bashful Buzzard. Mama dreams of someday becoming a master of the culinary arts, but her aspirations are often dashed because of family troubles. She lives with her son Beaky in a cave on top of a mountain located in Jones Valley. Mama usually abuses Beaky for being too stupid to remember anything at all, but sometimes acts nicer towards him only for her personal gain.

Nils Niedhart

 * Voice Actor: Arnold Schwarzenegger

Niedhart has long blonde hair and an extremely ripped body, usually wearing nothing but black pumps and a pink speedo with a German accent similar to his voice actor. His vindictive arrogance and superiority complex make him intolerably unlikeable, and his design is mostly the same as the 2020 reboot of Animaniacs, but altered to better fit the series. Niedhart claims himself to be the best at almost everything he does; he is actually a buffoonish cheat that will do anything to win at any means necessary. He does not even reside in Acme Acres, often visting as a Very Important Person. Niedhart's public image is rather controversial as the other Tunes don't like him at all.

Dire Commander Starbox

 * Voice Actor: Chris Sanders

Starbox is a dark grey Grimloxian of pint-size stature and a figure that makes seem like he's permanently starving. He has become much more villainous and criminally insane than ever, with his design having been heavily de-formed from the 2020 reboot of Animaniacs to make him even more grotesque. Starbox is the tyrannical commander of the Grimlox, a legion of planet-eating warlords constantly on the hunt for their next entree without any concern for the inhabitants. His race has no exact fixed home world as they have attempted to devour many potential homes in the past. Starbox's main nemesis is Gabriella, whose planet he devoured sometime before. As of Invader Starbox, he is currently being held in the clutches of a naïve human girl by the name of Cindy.

Cindy

 * Voice Actor: Eleanor Johnson

Cindy is a fairly young girl with ginger hair that wears a blue nightgown with white socks and black Mary Jane shoes. Her affectionate nature has been slightly toned down from her previous portrayal, but her design is still similar to the 2020 reboot of Animaniacs, but revamped to make her more suitable for the series. Cindy is sweet-natured towards everyone around her but has a reputation for being a bit overbearing towards the small and defenceless. She lives a homely little suburban abode with her younger sister Mindy, and their mother and father. Cindy is currently the supposed captor of Starbox, who is currently trying to escape her grasp.

Egwind

 * Voice Actor: Chris Cox

Egwind is an off-white laboratory mouse who is rather pudgy. He still suffers from constant misfortune despite his good nature, due to being seen as a useless third wheel, with his design staying familiar to the 2020 reboot of Animaniacs, albeit with minor improvements. Egwind is sometimes recruited by Pinky and the Brain to join them on their quest for dominance, but his bad luck usually renders him absent. He can sometimes be seen inside the very same cage that his fellow mice reside in. Egwind usually gets along with both Pinky and the Brain as they both are interested in the concept of a trio.

Jay-Pac le East tha Rapper

 * Voice Actor: Zeno Robinson

Jay-Pac is of African American descent, has a short goatee beard and is clad in the typical attire of the modern-day rapper. He seems to be much more respectfully prideful, and his design got slightly altered from the 2020 reboot of Animaniacs to better fit the series' more retro tone. Jay-Pac is highly acclaimed all over Acme Acres for his methods of creating fresh tracks to go with his rhymes but can sometimes be seen as someone who prefers the new over the old. He claims to have originated from New Freleng City but is hardly ever found there. Jay-Pac is quick to diss anyone who criticizes his rapping yet will instantly apologise for his earlier arrogance.

Doctor Moroni Frankenbeans

 * Voice Actor: Tom Kenny

Despite having a massive brain, Frankenbeans is of a rather short stature, along with pale green skin, Karloff-esque eyebrows, and a white labcoat. His macabre and dour tendencies still remain present at the cost of his villainy, but his design remains the same as his only appearance in 1952's Water, Water Every Hare, albeit with a few tweaks to fit the style of the series. Frankenbeans acts as the pyrotechnician of Schlesingerwood Studios, constantly supplying the special effects the studio needs to make their films bombastic. He is usually found in an old timey stall that acts as his personal workshop but is said to secretly lurk in an abandoned castle. Frankenbeans is seldom seen with the studio staff, preferring to keep himself to himself.

Brie

 * Voice Actor: Grey DeLisle

Brie has dark ginger hair tied in a ponytail and wears a purple suit with round glasses. She still retains her nerdy and subservient personality, but also now has an affinity for cheese; her design was overhauled in comparison to the 2020 reboot of Animaniacs. Brie mostly enjoys her position as a humble desk clerk, but sometimes gets harried by her unseen superiors. Because she only appears in The Great Warner Caper, her actual residence is currently unknown, but she can sometimes be seen in the Schlesingerwood Studios movie lot. Brie is on good terms with Bugs Tracy and Auguste DuPork, but is rather uncomfortable with the eccentricity of Dorlock Holmes.

Michael Benton a.k.a The Incredible Gnome in People's Mouths

 * Voice Actor: John DiMaggio

The Incredible Gnome is a small garden gnome with a white beard that wears a red cap. His irrepressible rage has become so immense that has completely drowned out his sense of morality, with his overall design being overhauled in comparison to the 2020 reboot of Animaniacs. Formerly an unscrupulous CEO by the name of Michael Benton, the Incredible Gnome now makes a living by abrasively speaking for those who can't speak for themselves. True to his occupation, he resides in the mouths of the residents of Acme Acres and pops out whenever they feel threatened. The Incredible Gnome claims to be doing his fellow Tunes a favour, but his shallow tendencies often ruin their lives, most evident with Porky Pig.

Norm McCabre

 * Voice Actor: Michael Douglas

McCabre is a light blue elderly ghost with a muscular physique that wears a green derby hat. He keeps his cranky but absent-minded personality from Looney Tunes Cartoons, with his design basically remaining unchanged from that series as well. McCabre usually doesn't like his eternal slumber disturbed by intruders but is willing help to out those in need. Because he only appears in The Great Warner Caper, his actual residence is currently unknown, being highly assumed to be somewhere in the Realm of Levitow. McCabre is also a close acquaintance of Bugs Tracy, Dorlock Holmes, and Auguste DuPork.

Butch "Killer" O'Reilly

 * Voice Actor: Alex Nussbaum

Butch has a brutish and opposing figure, wearing the cerulean and ochre jersey of his baseball team. He is now even more prone to cheating than his only appearance in 1946's Baseball Bugs, where his design takes inspiration from despite being heavily 40s-fied. Butch is the rough and tumble leader of the Gas-House Gorillas, a baseball team that is extremely proud of their ill-gained successes. Dur to the Gorillas being a visiting team, he does not even reside in Acme Acres. He is best friends with fellow Gorillas Mad Dog McNulty and Lil' Joe Johnson and the trio will often bully those who even dare to step up to them; out of the three, he is the least likely to concede defeat.

Mad Dog McNulty

 * Voice Actor: Vargus Mason

McNulty is of mixed heritage and has a rather flabby figure, wearing the cerulean and ochre jersey of his baseball team with a brown derby hat. He seems to be more sensitive and emotional than his only appearance in 1946's Baseball Bugs, where his design takes inspiration from despite being heavily 40s-fied. McNulty tends to be the weakest member of the Gas-House Gorillas due to having the tendency bawl and weep at the drop of a hat. Dur to the Gorillas being a visiting team, he does not even reside in Acme Acres. He is best friends with fellow Gorillas Butch "Killer" O'Reilly and Lil' Joe Johnson and the trio will often bully those who even dare to step up to them; out of the three, he is the most likely to concede defeat.

Lil' Joe Johnson

 * Voice Actor: Jamieson Price

Due to being the largest of his team, Johnson is possibly more ripped than Niedhart and wears the cerulean and ochre uniform of his baseball team, only with the cap obscuring his eyes. He is still as skilled on the field as before, but now only communicates in grunts in growls, with his 40s' esque design being based on the physique of famous baseball player Babe Ruth. As the star player of the Gas-House Gorillas, Johnson is famed for his technique of using his bat as a defensive shield. Dur to the Gorillas being a visiting team, he does not even reside in Acme Acres. He is best friends with fellow Gorillas Butch "Killer" O'Reilly and Mad Dog McNulty and the trio will often bully those who even dare to step up to them; out of the three, he is able to concede to both success and defeat.

Dwayne LaPistol

 * Voice Actor: Bernardo de Paula

LaPistol is rather skinny, wears the attire of a shady businessman, and has two buck teeth similar to that of a bunny. He still keeps his unnerving obsession over rabbits and bottoms, and his design is slighly altered from the 2020 reboot of Animaniacs. LaPistol has recently made a living for himself as Acme Acres' most reliable lawyer, but he has recently been subject to controversy over his "intellectual pursuits". His base of operations is an insurance company located in New Freleng City. LaPistol's behaviour often raises the irritation of Bugs Bunny, who ironically recommended him to Porky Pig when he was accused of alleged property theft.

O'Leo

 * Voice Actor: Ciaran Hinds

O'Leo is a leprechaun with ginger hair that is clad in typical leprechaun attire. His paranoia over losing his pot of gold is replaced with a more threatening personality, but his design basically remains unchanged from Looney Tunes Cartoons. O'Leo usually likes to strike fear into his targets through intimidation and a little Irish magic. Because he only appears in The Great Warner Caper, his actual residence is currently unknown, being highly assumed to be somewhere in Clampett Forest. In said episode, O'Leo acts as one of Buddy's enforcers alongside Al CaPants and the Monkeybird and resents Bugs Tracy for striking it big with his pot of gold.

Al CaPants

 * Voice Actor: Mitchell Whitfield

CaPants is a living pair of dark blue pants with white buttons that act as his eyes while the pockets function as his arms. His personality has become more fleshed out in comparison to Looney Tunes Cartoons, but his design basically remains unchanged from that series. CaPants lives in permanent fear of being worn as an accessory, and he has a habit of trash talking that makes him unbelievably annoying. Because he only appears in The Great Warner Caper, his actual residence is currently unknown, being highly assumed to be somewhere in New Freleng City. In said episode, CaPants acts as one of Buddy's enforcers alongside O'Leo and the Monkeybird and has vowed revenge against Auguste DuPork for donating him to charity.

Grumpy Goon

 * Voice Actor: Lewis Black

The Grumpy Goon has a physique so immensely muscular that his main attire of green waistcoat and trousers no longer fits him. He still retains his incurable anger issues and fascination with poetry, and his design basically remains unchanged from Looney Tunes Cartoons. The Grumpy Goon might seem like a nasty, spiteful bully at first glance, but he actually has a much softer side he would never reveal to anyone. Because he only appears in The Great Warner Caper, his actual residence is currently unknown, being highly assumed to be somewhere in Tashlinville. In said episode, O'Leo acts as Buddy's bodyguard.

Monkeybird

 * Voice Actor: Peter Mensah

The Monkeybird is a legendary purple behemoth that is a grotesque cross-hybrid between a gorilla and a parrot. He is now more villainously noble than just lovesick, but his design basically remains unchanged from Looney Tunes Cartoons. The Monkeybird is feared throughout Acme Acres for his combined superiority in fighting and flying, but he prefers to let others do the hard work. Because he only appears in The Great Warner Caper, his actual residence is currently unknown, being highly assumed to be somewhere in Larriva Jungle. In said episode, The Monkeybird acts as one of Buddy's enforcers alongside O'Leo and Al CaPants and has split from Dorlock Holmes since he rejected him at the altar.

Nickelwise the Dancing Clown

 * Voice Actor: Peter Stormare

Nickelwise is a shape-shifting deity whose appearance is an obvious cash-in on Pennywise from It. His personality is still as fear-mongering and sadistic as before, but his design was heavily altered from the 2020 reboot of Animaniacs to be less unappealing. Nickelwise is said to prey upon the fears of everyone in Acme Acres, manifesting them to abuse them into his will. He lurks in a gutter that is hidden deep within the Realm of Levitow. Almost the entire cast is extremely terrified of Nickelwise, especially Wakko Warner since he has an immense hatred for clowns.