Who Framed Roger Rabbit 2

The Walt Disney Company

 * Mickey Mouse
 * Minnie Mouse
 * Donald Duck
 * Daisy Duck
 * Goofy
 * Pluto
 * Pinocchio and Jiminy Cricket from Pinocchio
 * Winnie the Pooh and Tigger from the Winnie the Pooh series
 * Pepper Ann

Warner Bros.

 * Bugs Bunny
 * Daffy Duck
 * Elmer Fudd
 * Porky Pig
 * Yosemite Sam
 * Tweety Bird
 * Sylvester
 * Foghorn Leghorn
 * Road Runner
 * Wile E. Coyote
 * Marvin the Martian
 * Speedy Gonzales

Cartoon Network

 * Dexter and Dee Dee from Dexter's Laboratory
 * Blossom, Buttercup, and Bubbles from The Powerpuff Girls
 * Robot Jones from Whatever Happened to... Robot Jones?
 * Frankie Foster from Foster's Home for Imaginary Friends
 * K.O. from OK K.O.! Let's Be Heroes

MGM Cartoons

 * Droopy Dog
 * Tom and Jerry
 * Barney Bear
 * George and Junior
 * Screwy Squirrel

UPA

 * Dick Tracy
 * Mr. Magoo

Hanna-Barbera

 * Huckleberry Hound
 * Fred Flintstone, Barney Rubbles, and Dino from The Flintstones
 * Yogi Bear
 * Snagglepuss

Walter Lantz Productions

 * Woody Woodpecker

Jay Ward Productions

 * Rocky the Flying Squirrel
 * Bullwinkle J. Moose

Fleischer Studios

 * Betty Boop
 * Koko the Clown

Famous Studios

 * Popeye, Bluto, Olive Oyl, and Wimpy

Western Publishing

 * Little Lulu

Harvey Films

 * Casper the Friendly Ghost
 * Little Audrey
 * Baby Huey

Nickelodeon

 * Jenny Wakeman from My Life as a Teenage Robot

DePatie-Freleng Enterprises

 * The Pink Panther
 * The Ant and the Aardvark

Tezuka Productions

 * Astro Boy and Astro Girl
 * Kimba the White Lion
 * Princess Knight

PBS Kids

 * WordGirl

Internet characters

 * Matchu, Amber, and Famine from LimeTH
 * Zingo and Boppo from JoeyWaggoner
 * Zachery, Mike, Liza, Diana, and Jeffrey from Group 5
 * Judy Herrison and Cybil Herrison from Kyleboy21
 * Mugman
 * Bellybutton and Jellybean from Doodle Toons
 * Hatboy and Hatgirl
 * Lucy from Crash Zoom
 * Natalie from MatthewPrower
 * Lottie and Melissa from JJSponge120
 * Haleyu from MetalHeadMorgan
 * Parum and Merksia from Parum333
 * Autumn the Fox from Lazu-Arts